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Integrated proteome and phosphoproteome analyses of peripheral blood mononuclear cells in primary Sjögren syndrome patients

Primary Sjögren syndrome (pSS) is a common autoimmune disease. Here, we performed the first proteome and phosphoproteome analyses of peripheral blood mononuclear cells in pSS patients to obtain a comprehensive profile and identify the potential crucial proteins and pathways for the screening and eva...

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Autores principales: Huang, Shaoying, Zheng, Fengping, Liu, Lixiong, Meng, Shuhui, Cai, Wanxia, Zhang, Cantong, Dai, Weier, Liu, Dongzhou, Hong, Xiaoping, Tang, Donge, Dai, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835054/
https://www.ncbi.nlm.nih.gov/pubmed/33290261
http://dx.doi.org/10.18632/aging.202233
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author Huang, Shaoying
Zheng, Fengping
Liu, Lixiong
Meng, Shuhui
Cai, Wanxia
Zhang, Cantong
Dai, Weier
Liu, Dongzhou
Hong, Xiaoping
Tang, Donge
Dai, Yong
author_facet Huang, Shaoying
Zheng, Fengping
Liu, Lixiong
Meng, Shuhui
Cai, Wanxia
Zhang, Cantong
Dai, Weier
Liu, Dongzhou
Hong, Xiaoping
Tang, Donge
Dai, Yong
author_sort Huang, Shaoying
collection PubMed
description Primary Sjögren syndrome (pSS) is a common autoimmune disease. Here, we performed the first proteome and phosphoproteome analyses of peripheral blood mononuclear cells in pSS patients to obtain a comprehensive profile and identify the potential crucial proteins and pathways for the screening and evaluation of pSS patients. Peripheral blood mononuclear cells from 8 pSS-confirmed patients (American-European Consensus Group Criteria, 2002) and 10 normal controls were selected. Label-free quantitative proteomics was utilized to obtain quantitative information. In total, 787 proteins were identified as differentially expressed proteins, and 175 phosphosites on 123 proteins were identified as differentially phosphorylated proteins. We performed functional enrichment analyses with these proteins and phosphoproteins based on public database. Furthermore, protein-protein interaction network analyses were performed by using multiple algorithms. Using module and hub protein analyses, we identified 16 modules for the proteins, 2 clusters for the phosphoproteins and selected the top 10 hub proteins. Finally, we identified 22 motifs using motif analysis of the phosphosites and found 17 newly identified motifs, while 6 motifs were experimentally verified for known protein kinases. The findings distinguished pSS patients from normal controls at the peripheral blood mononuclear cells level and revealed potential candidates for use in pSS diagnosis.
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spelling pubmed-78350542021-02-03 Integrated proteome and phosphoproteome analyses of peripheral blood mononuclear cells in primary Sjögren syndrome patients Huang, Shaoying Zheng, Fengping Liu, Lixiong Meng, Shuhui Cai, Wanxia Zhang, Cantong Dai, Weier Liu, Dongzhou Hong, Xiaoping Tang, Donge Dai, Yong Aging (Albany NY) Research Paper Primary Sjögren syndrome (pSS) is a common autoimmune disease. Here, we performed the first proteome and phosphoproteome analyses of peripheral blood mononuclear cells in pSS patients to obtain a comprehensive profile and identify the potential crucial proteins and pathways for the screening and evaluation of pSS patients. Peripheral blood mononuclear cells from 8 pSS-confirmed patients (American-European Consensus Group Criteria, 2002) and 10 normal controls were selected. Label-free quantitative proteomics was utilized to obtain quantitative information. In total, 787 proteins were identified as differentially expressed proteins, and 175 phosphosites on 123 proteins were identified as differentially phosphorylated proteins. We performed functional enrichment analyses with these proteins and phosphoproteins based on public database. Furthermore, protein-protein interaction network analyses were performed by using multiple algorithms. Using module and hub protein analyses, we identified 16 modules for the proteins, 2 clusters for the phosphoproteins and selected the top 10 hub proteins. Finally, we identified 22 motifs using motif analysis of the phosphosites and found 17 newly identified motifs, while 6 motifs were experimentally verified for known protein kinases. The findings distinguished pSS patients from normal controls at the peripheral blood mononuclear cells level and revealed potential candidates for use in pSS diagnosis. Impact Journals 2020-12-03 /pmc/articles/PMC7835054/ /pubmed/33290261 http://dx.doi.org/10.18632/aging.202233 Text en Copyright: © 2020 Huang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Huang, Shaoying
Zheng, Fengping
Liu, Lixiong
Meng, Shuhui
Cai, Wanxia
Zhang, Cantong
Dai, Weier
Liu, Dongzhou
Hong, Xiaoping
Tang, Donge
Dai, Yong
Integrated proteome and phosphoproteome analyses of peripheral blood mononuclear cells in primary Sjögren syndrome patients
title Integrated proteome and phosphoproteome analyses of peripheral blood mononuclear cells in primary Sjögren syndrome patients
title_full Integrated proteome and phosphoproteome analyses of peripheral blood mononuclear cells in primary Sjögren syndrome patients
title_fullStr Integrated proteome and phosphoproteome analyses of peripheral blood mononuclear cells in primary Sjögren syndrome patients
title_full_unstemmed Integrated proteome and phosphoproteome analyses of peripheral blood mononuclear cells in primary Sjögren syndrome patients
title_short Integrated proteome and phosphoproteome analyses of peripheral blood mononuclear cells in primary Sjögren syndrome patients
title_sort integrated proteome and phosphoproteome analyses of peripheral blood mononuclear cells in primary sjögren syndrome patients
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835054/
https://www.ncbi.nlm.nih.gov/pubmed/33290261
http://dx.doi.org/10.18632/aging.202233
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