Generation of WAe001-A-58 human embryonic stem cell line with inducible expression of the SARS-CoV-2 nucleocapsid protein

Excessive prostaglandin E(2) (PGE(2)) is the key pathological basis for COVID-19 and a Celebrex treatment of hospitalized COVID-19 patients with comorbidities led to 100% discharged rate and zero death (Hong et al. 2020). It is also suggested that SARS-CoV-2 infected multiple organs and the SARS-CoV...

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Autores principales: Wu, Feima, Chen, Yan, You, Kai, Tan, Shenglin, Xu, Yingying, Wang, Pei-Hui, Liu, Shiming, Li, Yin-xiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 2021
Materias:
Lab resource: Stem Cell Line
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835078/
https://www.ncbi.nlm.nih.gov/pubmed/33771478
http://dx.doi.org/10.1016/j.scr.2021.102197
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author Wu, Feima
Chen, Yan
You, Kai
Tan, Shenglin
Xu, Yingying
Wang, Pei-Hui
Liu, Shiming
Li, Yin-xiong
author_facet Wu, Feima
Chen, Yan
You, Kai
Tan, Shenglin
Xu, Yingying
Wang, Pei-Hui
Liu, Shiming
Li, Yin-xiong
author_sort Wu, Feima
collection PubMed
description Excessive prostaglandin E(2) (PGE(2)) is the key pathological basis for COVID-19 and a Celebrex treatment of hospitalized COVID-19 patients with comorbidities led to 100% discharged rate and zero death (Hong et al. 2020). It is also suggested that SARS-CoV-2 infected multiple organs and the SARS-CoV nucleocapsid (N) protein transcriptionally drives the expression of the host COX-2 gene. In order to test whether SARS-CoV-2 N protein activates COX-2 transcription in multiple human relevant cell types, an expression inducible human embryonic stem cell line was generated by piggyBac transposon system. This cell line maintained its pluripotency, differentiation potentials, normal morphology and karyotype.
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spelling pubmed-78350782021-01-26 Generation of WAe001-A-58 human embryonic stem cell line with inducible expression of the SARS-CoV-2 nucleocapsid protein Wu, Feima Chen, Yan You, Kai Tan, Shenglin Xu, Yingying Wang, Pei-Hui Liu, Shiming Li, Yin-xiong Stem Cell Res Lab resource: Stem Cell Line Excessive prostaglandin E(2) (PGE(2)) is the key pathological basis for COVID-19 and a Celebrex treatment of hospitalized COVID-19 patients with comorbidities led to 100% discharged rate and zero death (Hong et al. 2020). It is also suggested that SARS-CoV-2 infected multiple organs and the SARS-CoV nucleocapsid (N) protein transcriptionally drives the expression of the host COX-2 gene. In order to test whether SARS-CoV-2 N protein activates COX-2 transcription in multiple human relevant cell types, an expression inducible human embryonic stem cell line was generated by piggyBac transposon system. This cell line maintained its pluripotency, differentiation potentials, normal morphology and karyotype. Published by Elsevier B.V. 2021-05 2021-01-26 /pmc/articles/PMC7835078/ /pubmed/33771478 http://dx.doi.org/10.1016/j.scr.2021.102197 Text en © 2021 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Lab resource: Stem Cell Line
Wu, Feima
Chen, Yan
You, Kai
Tan, Shenglin
Xu, Yingying
Wang, Pei-Hui
Liu, Shiming
Li, Yin-xiong
Generation of WAe001-A-58 human embryonic stem cell line with inducible expression of the SARS-CoV-2 nucleocapsid protein
title Generation of WAe001-A-58 human embryonic stem cell line with inducible expression of the SARS-CoV-2 nucleocapsid protein
title_full Generation of WAe001-A-58 human embryonic stem cell line with inducible expression of the SARS-CoV-2 nucleocapsid protein
title_fullStr Generation of WAe001-A-58 human embryonic stem cell line with inducible expression of the SARS-CoV-2 nucleocapsid protein
title_full_unstemmed Generation of WAe001-A-58 human embryonic stem cell line with inducible expression of the SARS-CoV-2 nucleocapsid protein
title_short Generation of WAe001-A-58 human embryonic stem cell line with inducible expression of the SARS-CoV-2 nucleocapsid protein
title_sort generation of wae001-a-58 human embryonic stem cell line with inducible expression of the sars-cov-2 nucleocapsid protein
topic Lab resource: Stem Cell Line
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835078/
https://www.ncbi.nlm.nih.gov/pubmed/33771478
http://dx.doi.org/10.1016/j.scr.2021.102197
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