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GC-MS/MS analysis of metabolites derived from a single human blastocyst

INTRODUCTION: The field of assisted reproductive technology (ART) has significantly advanced; however, morphological evaluation remains as the chosen method of assessment of embryo quality. OBJECTIVE: We aimed to examine metabolic changes in embryo culture medium to develop a non-invasive method for...

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Autores principales: Inoue, Naomi, Nishida, Yoshihiro, Harada, Emi, Sakai, Kumiko, Narahara, Hisashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835178/
https://www.ncbi.nlm.nih.gov/pubmed/33495963
http://dx.doi.org/10.1007/s11306-021-01770-x
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author Inoue, Naomi
Nishida, Yoshihiro
Harada, Emi
Sakai, Kumiko
Narahara, Hisashi
author_facet Inoue, Naomi
Nishida, Yoshihiro
Harada, Emi
Sakai, Kumiko
Narahara, Hisashi
author_sort Inoue, Naomi
collection PubMed
description INTRODUCTION: The field of assisted reproductive technology (ART) has significantly advanced; however, morphological evaluation remains as the chosen method of assessment of embryo quality. OBJECTIVE: We aimed to examine metabolic changes in embryo culture medium to develop a non-invasive method for evaluation of embryo quality. METHODS: We performed metabolic analysis of culture medium obtained from a single blastocyst cultured for freezing. RESULTS: In total, 187 (39.8%) of the 469 detectable organic acid metabolites were identified. A significant change (p < 0.05) was observed in eight metabolites between the good-quality and poor-quality embryo groups. Differences were observed in several metabolic pathways between the good-quality and poor-quality embryo groups. Metabolites that showed significant changes were primarily involved in the metabolism of branched-chain amino acids. CONCLUSION: The quantification of metabolism in human embryos may assist in identification and selection of good-quality embryos with high rates of survival before freezing and implantation in conjunction with morphological classification. This may help to identify embryos with high rates of survival. SUPPLEMENTARY INFORMATION: The online version of this article (10.1007/s11306-021-01770-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-78351782021-01-29 GC-MS/MS analysis of metabolites derived from a single human blastocyst Inoue, Naomi Nishida, Yoshihiro Harada, Emi Sakai, Kumiko Narahara, Hisashi Metabolomics Original Article INTRODUCTION: The field of assisted reproductive technology (ART) has significantly advanced; however, morphological evaluation remains as the chosen method of assessment of embryo quality. OBJECTIVE: We aimed to examine metabolic changes in embryo culture medium to develop a non-invasive method for evaluation of embryo quality. METHODS: We performed metabolic analysis of culture medium obtained from a single blastocyst cultured for freezing. RESULTS: In total, 187 (39.8%) of the 469 detectable organic acid metabolites were identified. A significant change (p < 0.05) was observed in eight metabolites between the good-quality and poor-quality embryo groups. Differences were observed in several metabolic pathways between the good-quality and poor-quality embryo groups. Metabolites that showed significant changes were primarily involved in the metabolism of branched-chain amino acids. CONCLUSION: The quantification of metabolism in human embryos may assist in identification and selection of good-quality embryos with high rates of survival before freezing and implantation in conjunction with morphological classification. This may help to identify embryos with high rates of survival. SUPPLEMENTARY INFORMATION: The online version of this article (10.1007/s11306-021-01770-x) contains supplementary material, which is available to authorized users. Springer US 2021-01-25 2021 /pmc/articles/PMC7835178/ /pubmed/33495963 http://dx.doi.org/10.1007/s11306-021-01770-x Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Inoue, Naomi
Nishida, Yoshihiro
Harada, Emi
Sakai, Kumiko
Narahara, Hisashi
GC-MS/MS analysis of metabolites derived from a single human blastocyst
title GC-MS/MS analysis of metabolites derived from a single human blastocyst
title_full GC-MS/MS analysis of metabolites derived from a single human blastocyst
title_fullStr GC-MS/MS analysis of metabolites derived from a single human blastocyst
title_full_unstemmed GC-MS/MS analysis of metabolites derived from a single human blastocyst
title_short GC-MS/MS analysis of metabolites derived from a single human blastocyst
title_sort gc-ms/ms analysis of metabolites derived from a single human blastocyst
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835178/
https://www.ncbi.nlm.nih.gov/pubmed/33495963
http://dx.doi.org/10.1007/s11306-021-01770-x
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