Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules

Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mo...

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Autores principales: Royo, Felix, Azkargorta, Mikel, Lavin, Jose L., Clos-Garcia, Marc, Cortazar, Ana R., Gonzalez-Lopez, Monika, Barcena, Laura, del Portillo, Hernando A., Yáñez-Mó, María, Marcilla, Antonio, Borras, Francesc E., Peinado, Hector, Guerrero, Isabel, Váles-Gómez, Mar, Cereijo, Unai, Sardon, Teresa, Aransay, Ana M., Elortza, Felix, Falcon-Perez, Juan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835421/
https://www.ncbi.nlm.nih.gov/pubmed/33511119
http://dx.doi.org/10.3389/fcell.2020.613583
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author Royo, Felix
Azkargorta, Mikel
Lavin, Jose L.
Clos-Garcia, Marc
Cortazar, Ana R.
Gonzalez-Lopez, Monika
Barcena, Laura
del Portillo, Hernando A.
Yáñez-Mó, María
Marcilla, Antonio
Borras, Francesc E.
Peinado, Hector
Guerrero, Isabel
Váles-Gómez, Mar
Cereijo, Unai
Sardon, Teresa
Aransay, Ana M.
Elortza, Felix
Falcon-Perez, Juan M.
author_facet Royo, Felix
Azkargorta, Mikel
Lavin, Jose L.
Clos-Garcia, Marc
Cortazar, Ana R.
Gonzalez-Lopez, Monika
Barcena, Laura
del Portillo, Hernando A.
Yáñez-Mó, María
Marcilla, Antonio
Borras, Francesc E.
Peinado, Hector
Guerrero, Isabel
Váles-Gómez, Mar
Cereijo, Unai
Sardon, Teresa
Aransay, Ana M.
Elortza, Felix
Falcon-Perez, Juan M.
author_sort Royo, Felix
collection PubMed
description Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-related response molecules, suggesting the activation of immune system signaling.
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spelling pubmed-78354212021-01-27 Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules Royo, Felix Azkargorta, Mikel Lavin, Jose L. Clos-Garcia, Marc Cortazar, Ana R. Gonzalez-Lopez, Monika Barcena, Laura del Portillo, Hernando A. Yáñez-Mó, María Marcilla, Antonio Borras, Francesc E. Peinado, Hector Guerrero, Isabel Váles-Gómez, Mar Cereijo, Unai Sardon, Teresa Aransay, Ana M. Elortza, Felix Falcon-Perez, Juan M. Front Cell Dev Biol Cell and Developmental Biology Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-related response molecules, suggesting the activation of immune system signaling. Frontiers Media S.A. 2021-01-12 /pmc/articles/PMC7835421/ /pubmed/33511119 http://dx.doi.org/10.3389/fcell.2020.613583 Text en Copyright © 2021 Royo, Azkargorta, Lavin, Clos-Garcia, Cortazar, Gonzalez-Lopez, Barcena, del Portillo, Yáñez-Mó, Marcilla, Borras, Peinado, Guerrero, Váles-Gómez, Cereijo, Sardon, Aransay, Elortza and Falcon-Perez. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Royo, Felix
Azkargorta, Mikel
Lavin, Jose L.
Clos-Garcia, Marc
Cortazar, Ana R.
Gonzalez-Lopez, Monika
Barcena, Laura
del Portillo, Hernando A.
Yáñez-Mó, María
Marcilla, Antonio
Borras, Francesc E.
Peinado, Hector
Guerrero, Isabel
Váles-Gómez, Mar
Cereijo, Unai
Sardon, Teresa
Aransay, Ana M.
Elortza, Felix
Falcon-Perez, Juan M.
Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
title Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
title_full Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
title_fullStr Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
title_full_unstemmed Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
title_short Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
title_sort extracellular vesicles from liver progenitor cells downregulates fibroblast metabolic activity and increase the expression of immune-response related molecules
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835421/
https://www.ncbi.nlm.nih.gov/pubmed/33511119
http://dx.doi.org/10.3389/fcell.2020.613583
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