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ISEc69-Mediated Mobilization of the Colistin Resistance Gene mcr-2 in Escherichia coli

OBJECTIVES: The emergence of mobile colistin resistance genes has compromised the efficacy of the last resort antibiotic, colistin, in clinical treatment. The mcr-2 gene was first identified in Belgium in association with the insertion sequence ISEc69. However, the molecular mechanisms of mcr-2 mobi...

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Autores principales: He, Yu-Zhang, Long, Teng-Fei, He, Bing, Li, Xing-Ping, Li, Gong, Chen, Liang, Liao, Xiao-Ping, Liu, Ya-Hong, Sun, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835479/
https://www.ncbi.nlm.nih.gov/pubmed/33510713
http://dx.doi.org/10.3389/fmicb.2020.564973
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author He, Yu-Zhang
Long, Teng-Fei
He, Bing
Li, Xing-Ping
Li, Gong
Chen, Liang
Liao, Xiao-Ping
Liu, Ya-Hong
Sun, Jian
author_facet He, Yu-Zhang
Long, Teng-Fei
He, Bing
Li, Xing-Ping
Li, Gong
Chen, Liang
Liao, Xiao-Ping
Liu, Ya-Hong
Sun, Jian
author_sort He, Yu-Zhang
collection PubMed
description OBJECTIVES: The emergence of mobile colistin resistance genes has compromised the efficacy of the last resort antibiotic, colistin, in clinical treatment. The mcr-2 gene was first identified in Belgium in association with the insertion sequence ISEc69. However, the molecular mechanisms of mcr-2 mobilization are not well understood. METHODS: To further explore the mobilization of mcr-2 gene via ISEc69, we constructed a conjugative plasmid that carries an intact composite transposon Tn7052. Transposition assays were performed by conjugation, the transposition sites were characterized by arbitrary primed PCR and DNA sequencing. RESULTS: In this study, we experimentally demonstrated that mcr-2 could be mobilized as a composite transposon Tn7052 and its transposition generated 8-bp AT-rich duplications in the host genome. CONCLUSION: These results indicate that mcr-2 gene could be mobilized by ISEc69, the current investigations provide mechanistic insights in the transposition of mcr-2.
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spelling pubmed-78354792021-01-27 ISEc69-Mediated Mobilization of the Colistin Resistance Gene mcr-2 in Escherichia coli He, Yu-Zhang Long, Teng-Fei He, Bing Li, Xing-Ping Li, Gong Chen, Liang Liao, Xiao-Ping Liu, Ya-Hong Sun, Jian Front Microbiol Microbiology OBJECTIVES: The emergence of mobile colistin resistance genes has compromised the efficacy of the last resort antibiotic, colistin, in clinical treatment. The mcr-2 gene was first identified in Belgium in association with the insertion sequence ISEc69. However, the molecular mechanisms of mcr-2 mobilization are not well understood. METHODS: To further explore the mobilization of mcr-2 gene via ISEc69, we constructed a conjugative plasmid that carries an intact composite transposon Tn7052. Transposition assays were performed by conjugation, the transposition sites were characterized by arbitrary primed PCR and DNA sequencing. RESULTS: In this study, we experimentally demonstrated that mcr-2 could be mobilized as a composite transposon Tn7052 and its transposition generated 8-bp AT-rich duplications in the host genome. CONCLUSION: These results indicate that mcr-2 gene could be mobilized by ISEc69, the current investigations provide mechanistic insights in the transposition of mcr-2. Frontiers Media S.A. 2021-01-12 /pmc/articles/PMC7835479/ /pubmed/33510713 http://dx.doi.org/10.3389/fmicb.2020.564973 Text en Copyright © 2021 He, Long, He, Li, Li, Chen, Liao, Liu and Sun. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
He, Yu-Zhang
Long, Teng-Fei
He, Bing
Li, Xing-Ping
Li, Gong
Chen, Liang
Liao, Xiao-Ping
Liu, Ya-Hong
Sun, Jian
ISEc69-Mediated Mobilization of the Colistin Resistance Gene mcr-2 in Escherichia coli
title ISEc69-Mediated Mobilization of the Colistin Resistance Gene mcr-2 in Escherichia coli
title_full ISEc69-Mediated Mobilization of the Colistin Resistance Gene mcr-2 in Escherichia coli
title_fullStr ISEc69-Mediated Mobilization of the Colistin Resistance Gene mcr-2 in Escherichia coli
title_full_unstemmed ISEc69-Mediated Mobilization of the Colistin Resistance Gene mcr-2 in Escherichia coli
title_short ISEc69-Mediated Mobilization of the Colistin Resistance Gene mcr-2 in Escherichia coli
title_sort isec69-mediated mobilization of the colistin resistance gene mcr-2 in escherichia coli
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835479/
https://www.ncbi.nlm.nih.gov/pubmed/33510713
http://dx.doi.org/10.3389/fmicb.2020.564973
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