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Heat-Induced Oxidation of the Nuclei and Cytosol

The concept that heat stress (HS) causes a large accumulation of reactive oxygen species (ROS) is widely accepted. However, the intracellular compartmentation of ROS accumulation has been poorly characterized. We therefore used redox-sensitive green fluorescent protein (roGFP2) to provide compartmen...

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Autores principales: Babbar, Richa, Karpinska, Barbara, Grover, Anil, Foyer, Christine H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835529/
https://www.ncbi.nlm.nih.gov/pubmed/33510759
http://dx.doi.org/10.3389/fpls.2020.617779
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author Babbar, Richa
Karpinska, Barbara
Grover, Anil
Foyer, Christine H.
author_facet Babbar, Richa
Karpinska, Barbara
Grover, Anil
Foyer, Christine H.
author_sort Babbar, Richa
collection PubMed
description The concept that heat stress (HS) causes a large accumulation of reactive oxygen species (ROS) is widely accepted. However, the intracellular compartmentation of ROS accumulation has been poorly characterized. We therefore used redox-sensitive green fluorescent protein (roGFP2) to provide compartment-specific information on heat-induced redox changes of the nuclei and cytosol of Arabidopsis leaf epidermal and stomatal guard cells. We show that HS causes a large increase in the degree of oxidation of both compartments, causing large shifts in the glutathione redox potentials of the cells. Heat-induced increases in the levels of the marker transcripts, heat shock protein (HSP)101, and ascorbate peroxidase (APX)2 were maximal after 15 min of the onset of the heat treatment. RNAseq analysis of the transcript profiles of the control and heat-treated seedlings revealed large changes in transcripts encoding HSPs, mitochondrial proteins, transcription factors, and other nuclear localized components. We conclude that HS causes extensive oxidation of the nucleus as well as the cytosol. We propose that the heat-induced changes in the nuclear redox state are central to both genetic and epigenetic control of plant responses to HS.
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spelling pubmed-78355292021-01-27 Heat-Induced Oxidation of the Nuclei and Cytosol Babbar, Richa Karpinska, Barbara Grover, Anil Foyer, Christine H. Front Plant Sci Plant Science The concept that heat stress (HS) causes a large accumulation of reactive oxygen species (ROS) is widely accepted. However, the intracellular compartmentation of ROS accumulation has been poorly characterized. We therefore used redox-sensitive green fluorescent protein (roGFP2) to provide compartment-specific information on heat-induced redox changes of the nuclei and cytosol of Arabidopsis leaf epidermal and stomatal guard cells. We show that HS causes a large increase in the degree of oxidation of both compartments, causing large shifts in the glutathione redox potentials of the cells. Heat-induced increases in the levels of the marker transcripts, heat shock protein (HSP)101, and ascorbate peroxidase (APX)2 were maximal after 15 min of the onset of the heat treatment. RNAseq analysis of the transcript profiles of the control and heat-treated seedlings revealed large changes in transcripts encoding HSPs, mitochondrial proteins, transcription factors, and other nuclear localized components. We conclude that HS causes extensive oxidation of the nucleus as well as the cytosol. We propose that the heat-induced changes in the nuclear redox state are central to both genetic and epigenetic control of plant responses to HS. Frontiers Media S.A. 2021-01-12 /pmc/articles/PMC7835529/ /pubmed/33510759 http://dx.doi.org/10.3389/fpls.2020.617779 Text en Copyright © 2021 Babbar, Karpinska, Grover and Foyer. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Babbar, Richa
Karpinska, Barbara
Grover, Anil
Foyer, Christine H.
Heat-Induced Oxidation of the Nuclei and Cytosol
title Heat-Induced Oxidation of the Nuclei and Cytosol
title_full Heat-Induced Oxidation of the Nuclei and Cytosol
title_fullStr Heat-Induced Oxidation of the Nuclei and Cytosol
title_full_unstemmed Heat-Induced Oxidation of the Nuclei and Cytosol
title_short Heat-Induced Oxidation of the Nuclei and Cytosol
title_sort heat-induced oxidation of the nuclei and cytosol
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835529/
https://www.ncbi.nlm.nih.gov/pubmed/33510759
http://dx.doi.org/10.3389/fpls.2020.617779
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