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Curcumin-Induced Global Profiling of Transcriptomes in Small Cell Lung Cancer Cells
BACKGROUND: Curcumin, one of the promising candidates for supplementary therapy in cancer treatment, has been demonstrated by numerous preclinical and clinical evidence to be beneficial in treating various cancers. Apart from the critical role in a deluge of pathological processes, some mRNAs, in pa...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835540/ https://www.ncbi.nlm.nih.gov/pubmed/33511113 http://dx.doi.org/10.3389/fcell.2020.588299 |
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author | Mo, Fei Xiao, Yinan Zeng, Hao Fan, Dian Song, Jinen Liu, Xiaobei Luo, Meng Ma, Xuelei |
author_facet | Mo, Fei Xiao, Yinan Zeng, Hao Fan, Dian Song, Jinen Liu, Xiaobei Luo, Meng Ma, Xuelei |
author_sort | Mo, Fei |
collection | PubMed |
description | BACKGROUND: Curcumin, one of the promising candidates for supplementary therapy in cancer treatment, has been demonstrated by numerous preclinical and clinical evidence to be beneficial in treating various cancers. Apart from the critical role in a deluge of pathological processes, some mRNAs, in particular, microRNAs (miRNAs), are also involved in the anti-tumor activity. Therefore, our research focused on the possible effects of curcumin on small cell lung cancer (SCLC) cells and drew a comprehensive transcriptomes profile by high throughput sequencing to understand the molecular mechanism of curcumin as an anti-tumor agent. METHODS: First, we calculated the apoptosis rate of H446 cells (a human SCLC cell line) cultured with curcumin. The high output sequencing uncovered the altered expression profile of genes and miRNAs. KEGG analysis selected the potential signal pathway associated with the antiproliferative property of curcumin. Finally, miRNAs significantly changed, as well as the regulatory roles of those miRNAs in cell apoptosis were determined. RESULT: The apoptosis rate of H446 cells increased under the elevated concentration of curcumin treatment. And cell cycle-related genes downregulated in the curcumin-treated cells. Besides, miRNA-548ah-5p of a high level acted as a negative role in the anticarcinogenic activity of curcumin. CONCLUSION: Our findings not only enriched the understanding of anti-tumor activity initiated by curcumin through figuring out the downregulated cell cycle-related pathways but also shed light on its novel therapeutic application. |
format | Online Article Text |
id | pubmed-7835540 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78355402021-01-27 Curcumin-Induced Global Profiling of Transcriptomes in Small Cell Lung Cancer Cells Mo, Fei Xiao, Yinan Zeng, Hao Fan, Dian Song, Jinen Liu, Xiaobei Luo, Meng Ma, Xuelei Front Cell Dev Biol Cell and Developmental Biology BACKGROUND: Curcumin, one of the promising candidates for supplementary therapy in cancer treatment, has been demonstrated by numerous preclinical and clinical evidence to be beneficial in treating various cancers. Apart from the critical role in a deluge of pathological processes, some mRNAs, in particular, microRNAs (miRNAs), are also involved in the anti-tumor activity. Therefore, our research focused on the possible effects of curcumin on small cell lung cancer (SCLC) cells and drew a comprehensive transcriptomes profile by high throughput sequencing to understand the molecular mechanism of curcumin as an anti-tumor agent. METHODS: First, we calculated the apoptosis rate of H446 cells (a human SCLC cell line) cultured with curcumin. The high output sequencing uncovered the altered expression profile of genes and miRNAs. KEGG analysis selected the potential signal pathway associated with the antiproliferative property of curcumin. Finally, miRNAs significantly changed, as well as the regulatory roles of those miRNAs in cell apoptosis were determined. RESULT: The apoptosis rate of H446 cells increased under the elevated concentration of curcumin treatment. And cell cycle-related genes downregulated in the curcumin-treated cells. Besides, miRNA-548ah-5p of a high level acted as a negative role in the anticarcinogenic activity of curcumin. CONCLUSION: Our findings not only enriched the understanding of anti-tumor activity initiated by curcumin through figuring out the downregulated cell cycle-related pathways but also shed light on its novel therapeutic application. Frontiers Media S.A. 2021-01-12 /pmc/articles/PMC7835540/ /pubmed/33511113 http://dx.doi.org/10.3389/fcell.2020.588299 Text en Copyright © 2021 Mo, Xiao, Zeng, Fan, Song, Liu, Luo and Ma. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Mo, Fei Xiao, Yinan Zeng, Hao Fan, Dian Song, Jinen Liu, Xiaobei Luo, Meng Ma, Xuelei Curcumin-Induced Global Profiling of Transcriptomes in Small Cell Lung Cancer Cells |
title | Curcumin-Induced Global Profiling of Transcriptomes in Small Cell Lung Cancer Cells |
title_full | Curcumin-Induced Global Profiling of Transcriptomes in Small Cell Lung Cancer Cells |
title_fullStr | Curcumin-Induced Global Profiling of Transcriptomes in Small Cell Lung Cancer Cells |
title_full_unstemmed | Curcumin-Induced Global Profiling of Transcriptomes in Small Cell Lung Cancer Cells |
title_short | Curcumin-Induced Global Profiling of Transcriptomes in Small Cell Lung Cancer Cells |
title_sort | curcumin-induced global profiling of transcriptomes in small cell lung cancer cells |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835540/ https://www.ncbi.nlm.nih.gov/pubmed/33511113 http://dx.doi.org/10.3389/fcell.2020.588299 |
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