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Physical Activity Level Influences MTHFR Gene Methylation Profile in Diabetic Patients

INTRODUCTION: MTHFR methylation status is associated with microvascular complications in diabetes, but the factors influencing this profile remain unknown. OBJECTIVE: The aim of this study was to evaluate the influence of physical activity level and nutritional status on the methylation profile of t...

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Autores principales: Diniz, Tainá Gomes, Silva, Alexandre Sérgio, dos Santos Nunes, Mayara Karla, Ribeiro, Mateus Duarte, Filho, João Modesto, do Nascimento, Rayner Anderson Ferreira, Gomes, Cecília Neta Alves Pegado, Evangelista, Isabella Wanderley de Queiroga, de Oliveira, Naila Francis Paulo, Persuhn, Darlene Camati
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835660/
https://www.ncbi.nlm.nih.gov/pubmed/33510650
http://dx.doi.org/10.3389/fphys.2020.618672
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author Diniz, Tainá Gomes
Silva, Alexandre Sérgio
dos Santos Nunes, Mayara Karla
Ribeiro, Mateus Duarte
Filho, João Modesto
do Nascimento, Rayner Anderson Ferreira
Gomes, Cecília Neta Alves Pegado
Evangelista, Isabella Wanderley de Queiroga
de Oliveira, Naila Francis Paulo
Persuhn, Darlene Camati
author_facet Diniz, Tainá Gomes
Silva, Alexandre Sérgio
dos Santos Nunes, Mayara Karla
Ribeiro, Mateus Duarte
Filho, João Modesto
do Nascimento, Rayner Anderson Ferreira
Gomes, Cecília Neta Alves Pegado
Evangelista, Isabella Wanderley de Queiroga
de Oliveira, Naila Francis Paulo
Persuhn, Darlene Camati
author_sort Diniz, Tainá Gomes
collection PubMed
description INTRODUCTION: MTHFR methylation status is associated with microvascular complications in diabetes, but the factors influencing this profile remain unknown. OBJECTIVE: The aim of this study was to evaluate the influence of physical activity level and nutritional status on the methylation profile of the MTHFR gene in patients with type 2 diabetes mellitus (T2DM). METHODS: A total of 111 patients, 43 men and 68 women diagnosed with DM (7.0 ± 2.3 years), answered the International Physical Activity Questionnaire (IPAQ) and underwent blood collection for biochemical analysis, DNA extraction, and MTHFR gene methylation profile determination. RESULT: The comparison of the methylation pattern showed that the partially methylated profile predominates in the insufficiently active group (85%), which does not occur in the sufficiently active group (54%) (p = 0.012). No differences were found in the nutritional status comparison. Logistic regression including overweight, waist circumference, gender, age, time of DM, hypertension, dyslipidemia, smoking, alcoholism, and family DM revealed that the association of the level of physical activity with methylation profile proved to be independent of these confounding variables. Considering the partially methylated profile as a result, being physically inactive favors the partially methylated MTHFR pattern in patients with DM. CONCLUSION: We concluded that insufficient physical activity is associated with partially methylated pattern of MTHFR promoter.
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spelling pubmed-78356602021-01-27 Physical Activity Level Influences MTHFR Gene Methylation Profile in Diabetic Patients Diniz, Tainá Gomes Silva, Alexandre Sérgio dos Santos Nunes, Mayara Karla Ribeiro, Mateus Duarte Filho, João Modesto do Nascimento, Rayner Anderson Ferreira Gomes, Cecília Neta Alves Pegado Evangelista, Isabella Wanderley de Queiroga de Oliveira, Naila Francis Paulo Persuhn, Darlene Camati Front Physiol Physiology INTRODUCTION: MTHFR methylation status is associated with microvascular complications in diabetes, but the factors influencing this profile remain unknown. OBJECTIVE: The aim of this study was to evaluate the influence of physical activity level and nutritional status on the methylation profile of the MTHFR gene in patients with type 2 diabetes mellitus (T2DM). METHODS: A total of 111 patients, 43 men and 68 women diagnosed with DM (7.0 ± 2.3 years), answered the International Physical Activity Questionnaire (IPAQ) and underwent blood collection for biochemical analysis, DNA extraction, and MTHFR gene methylation profile determination. RESULT: The comparison of the methylation pattern showed that the partially methylated profile predominates in the insufficiently active group (85%), which does not occur in the sufficiently active group (54%) (p = 0.012). No differences were found in the nutritional status comparison. Logistic regression including overweight, waist circumference, gender, age, time of DM, hypertension, dyslipidemia, smoking, alcoholism, and family DM revealed that the association of the level of physical activity with methylation profile proved to be independent of these confounding variables. Considering the partially methylated profile as a result, being physically inactive favors the partially methylated MTHFR pattern in patients with DM. CONCLUSION: We concluded that insufficient physical activity is associated with partially methylated pattern of MTHFR promoter. Frontiers Media S.A. 2021-01-12 /pmc/articles/PMC7835660/ /pubmed/33510650 http://dx.doi.org/10.3389/fphys.2020.618672 Text en Copyright © 2021 Diniz, Silva, dos Santos Nunes, Ribeiro, Filho, do Nascimento, Gomes, Evangelista, de Oliveira and Persuhn. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Diniz, Tainá Gomes
Silva, Alexandre Sérgio
dos Santos Nunes, Mayara Karla
Ribeiro, Mateus Duarte
Filho, João Modesto
do Nascimento, Rayner Anderson Ferreira
Gomes, Cecília Neta Alves Pegado
Evangelista, Isabella Wanderley de Queiroga
de Oliveira, Naila Francis Paulo
Persuhn, Darlene Camati
Physical Activity Level Influences MTHFR Gene Methylation Profile in Diabetic Patients
title Physical Activity Level Influences MTHFR Gene Methylation Profile in Diabetic Patients
title_full Physical Activity Level Influences MTHFR Gene Methylation Profile in Diabetic Patients
title_fullStr Physical Activity Level Influences MTHFR Gene Methylation Profile in Diabetic Patients
title_full_unstemmed Physical Activity Level Influences MTHFR Gene Methylation Profile in Diabetic Patients
title_short Physical Activity Level Influences MTHFR Gene Methylation Profile in Diabetic Patients
title_sort physical activity level influences mthfr gene methylation profile in diabetic patients
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835660/
https://www.ncbi.nlm.nih.gov/pubmed/33510650
http://dx.doi.org/10.3389/fphys.2020.618672
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