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Prediction of Calving to Conception Interval Length Using Algorithmic Analysis of Endometrial mRNA Expression in Bovine

SIMPLE SUMMARY: Our study aimed to develop the unsupervised clustering model based solely on selected markers to investigate the association between calving conception interval length, subclinical endometritis, and endometrial gene expression. An algorithmic analysis of endometrial gene expression s...

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Autores principales: Tobolski, Dawid, Łukasik, Karolina, Bacławska, Agnieszka, Skarżyński, Dariusz Jan, Hostens, Miel, Barański, Wojciech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835770/
https://www.ncbi.nlm.nih.gov/pubmed/33478124
http://dx.doi.org/10.3390/ani11010236
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author Tobolski, Dawid
Łukasik, Karolina
Bacławska, Agnieszka
Skarżyński, Dariusz Jan
Hostens, Miel
Barański, Wojciech
author_facet Tobolski, Dawid
Łukasik, Karolina
Bacławska, Agnieszka
Skarżyński, Dariusz Jan
Hostens, Miel
Barański, Wojciech
author_sort Tobolski, Dawid
collection PubMed
description SIMPLE SUMMARY: Our study aimed to develop the unsupervised clustering model based solely on selected markers to investigate the association between calving conception interval length, subclinical endometritis, and endometrial gene expression. An algorithmic analysis of endometrial gene expression showed a higher predictive ability to identify cows exhibiting excellent fertility than previously used methods, highlighting the correlation between INHBA/INHA and calving–conception interval length. ABSTRACT: After parturition, the uterus undergoes significant reconstruction, allows the endometrium to create an environment for subsequent embryo development. Here, we used an unsupervised algorithmic approach to select characteristic endometrial mRNA expression patterns of proposed markers and investigate each marker’s role as an individual indicator of reproductive success. Clinically healthy cows at a sixth week postpartum were examined, the percentage of neutrophils (PMNs%) in the cytological smear was calculated, and an endometrial biopsy was taken for qPCR. Based on pregnancy examination, cows were divided into three groups: Pregnant before 100 days postpartum (P100, n = 11), pregnant between 100–200-day (P200, n = 14), and culled (C, n = 10). Animals were also classified based on two PMNs% thresholds > 5% PMNs and > 10% PMNs. The expression of IL1B, IL6, CXCL8, and IL17A was higher in >10%PMNs. The expression of PTGS1 was higher in the P200 compared to P100. Upregulation of inhibin A subunit (INHA) and downregulation of inhibin β A subunit (INHBA) were observed in the P100. INHBA/INHA ratio was the most accurate linear predictor of the calving-to-conception interval. The application of the k-means algorithm allowed the identification of five unique expression patterns. The sensitivity and specificity of predicting allocation to P100 were 81% and 79%. We also documented the low efficiency of genes associated with subclinical endometritis and PMNs% in determining reproductive capability. These results suggested the presence of distinctive expression patterns in 6 weeks postpartum, correlated with cows’ reproductive capacity. Furthermore, we proposed the INHBA/INHA ratio as an indicator of calving-to-conception interval length.
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spelling pubmed-78357702021-01-27 Prediction of Calving to Conception Interval Length Using Algorithmic Analysis of Endometrial mRNA Expression in Bovine Tobolski, Dawid Łukasik, Karolina Bacławska, Agnieszka Skarżyński, Dariusz Jan Hostens, Miel Barański, Wojciech Animals (Basel) Article SIMPLE SUMMARY: Our study aimed to develop the unsupervised clustering model based solely on selected markers to investigate the association between calving conception interval length, subclinical endometritis, and endometrial gene expression. An algorithmic analysis of endometrial gene expression showed a higher predictive ability to identify cows exhibiting excellent fertility than previously used methods, highlighting the correlation between INHBA/INHA and calving–conception interval length. ABSTRACT: After parturition, the uterus undergoes significant reconstruction, allows the endometrium to create an environment for subsequent embryo development. Here, we used an unsupervised algorithmic approach to select characteristic endometrial mRNA expression patterns of proposed markers and investigate each marker’s role as an individual indicator of reproductive success. Clinically healthy cows at a sixth week postpartum were examined, the percentage of neutrophils (PMNs%) in the cytological smear was calculated, and an endometrial biopsy was taken for qPCR. Based on pregnancy examination, cows were divided into three groups: Pregnant before 100 days postpartum (P100, n = 11), pregnant between 100–200-day (P200, n = 14), and culled (C, n = 10). Animals were also classified based on two PMNs% thresholds > 5% PMNs and > 10% PMNs. The expression of IL1B, IL6, CXCL8, and IL17A was higher in >10%PMNs. The expression of PTGS1 was higher in the P200 compared to P100. Upregulation of inhibin A subunit (INHA) and downregulation of inhibin β A subunit (INHBA) were observed in the P100. INHBA/INHA ratio was the most accurate linear predictor of the calving-to-conception interval. The application of the k-means algorithm allowed the identification of five unique expression patterns. The sensitivity and specificity of predicting allocation to P100 were 81% and 79%. We also documented the low efficiency of genes associated with subclinical endometritis and PMNs% in determining reproductive capability. These results suggested the presence of distinctive expression patterns in 6 weeks postpartum, correlated with cows’ reproductive capacity. Furthermore, we proposed the INHBA/INHA ratio as an indicator of calving-to-conception interval length. MDPI 2021-01-19 /pmc/articles/PMC7835770/ /pubmed/33478124 http://dx.doi.org/10.3390/ani11010236 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tobolski, Dawid
Łukasik, Karolina
Bacławska, Agnieszka
Skarżyński, Dariusz Jan
Hostens, Miel
Barański, Wojciech
Prediction of Calving to Conception Interval Length Using Algorithmic Analysis of Endometrial mRNA Expression in Bovine
title Prediction of Calving to Conception Interval Length Using Algorithmic Analysis of Endometrial mRNA Expression in Bovine
title_full Prediction of Calving to Conception Interval Length Using Algorithmic Analysis of Endometrial mRNA Expression in Bovine
title_fullStr Prediction of Calving to Conception Interval Length Using Algorithmic Analysis of Endometrial mRNA Expression in Bovine
title_full_unstemmed Prediction of Calving to Conception Interval Length Using Algorithmic Analysis of Endometrial mRNA Expression in Bovine
title_short Prediction of Calving to Conception Interval Length Using Algorithmic Analysis of Endometrial mRNA Expression in Bovine
title_sort prediction of calving to conception interval length using algorithmic analysis of endometrial mrna expression in bovine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835770/
https://www.ncbi.nlm.nih.gov/pubmed/33478124
http://dx.doi.org/10.3390/ani11010236
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