Cargando…
Changes in the Splenic Melanomacrophage Centre Surface Area in Southern Bluefin Tuna (Thunnus maccoyii) Are Associated with Blood Fluke Infections
Melanomacrophage centres (MMCs) are aggregates of macrophages accumulating various pigments. They have been proposed as an indicator of fish immune response. Blood flukes are common parasites in farmed fish. Two cohorts of wild Southern Bluefin Tuna (Thunnus maccoyi) were examined at transfer, befor...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835979/ https://www.ncbi.nlm.nih.gov/pubmed/33477690 http://dx.doi.org/10.3390/pathogens10010079 |
_version_ | 1783642652215869440 |
---|---|
author | Nowak, Barbara F. Dang, Mai Webber, Claire Neumann, Lukas Bridle, Andrew Bermudez, Roberto Evans, Daryl |
author_facet | Nowak, Barbara F. Dang, Mai Webber, Claire Neumann, Lukas Bridle, Andrew Bermudez, Roberto Evans, Daryl |
author_sort | Nowak, Barbara F. |
collection | PubMed |
description | Melanomacrophage centres (MMCs) are aggregates of macrophages accumulating various pigments. They have been proposed as an indicator of fish immune response. Blood flukes are common parasites in farmed fish. Two cohorts of wild Southern Bluefin Tuna (Thunnus maccoyi) were examined at transfer, before treatment against blood flukes (pre-treatment) and at harvest. MMCs were assessed in histological sections using image analysis, while Cardicola forsteri and Cardicola orientalis infection severity was determined using qPCR, count of adult flukes in heart flushes and count of eggs in gill filaments. Fish from both cohorts showed the same pattern in the changes in the surface area of MMCs. The surface area of splenic MMCs increased over the ranching duration and was positively correlated to the PCR determined copy numbers of Cardicola forsteri ITS2 rDNA in the gills of those fish. However, the infection with blood fluke was more variable, both between cohorts and individuals within the same cohort. Eggs of blood fluke were detected in renal MMCs using histology. Cardicola forsteri had a higher prevalence than Cardicola orientalis. This study contributes to our understanding of blood fluke infections in Southern Bluefin Tuna and their interactions with MMCs. |
format | Online Article Text |
id | pubmed-7835979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78359792021-01-27 Changes in the Splenic Melanomacrophage Centre Surface Area in Southern Bluefin Tuna (Thunnus maccoyii) Are Associated with Blood Fluke Infections Nowak, Barbara F. Dang, Mai Webber, Claire Neumann, Lukas Bridle, Andrew Bermudez, Roberto Evans, Daryl Pathogens Article Melanomacrophage centres (MMCs) are aggregates of macrophages accumulating various pigments. They have been proposed as an indicator of fish immune response. Blood flukes are common parasites in farmed fish. Two cohorts of wild Southern Bluefin Tuna (Thunnus maccoyi) were examined at transfer, before treatment against blood flukes (pre-treatment) and at harvest. MMCs were assessed in histological sections using image analysis, while Cardicola forsteri and Cardicola orientalis infection severity was determined using qPCR, count of adult flukes in heart flushes and count of eggs in gill filaments. Fish from both cohorts showed the same pattern in the changes in the surface area of MMCs. The surface area of splenic MMCs increased over the ranching duration and was positively correlated to the PCR determined copy numbers of Cardicola forsteri ITS2 rDNA in the gills of those fish. However, the infection with blood fluke was more variable, both between cohorts and individuals within the same cohort. Eggs of blood fluke were detected in renal MMCs using histology. Cardicola forsteri had a higher prevalence than Cardicola orientalis. This study contributes to our understanding of blood fluke infections in Southern Bluefin Tuna and their interactions with MMCs. MDPI 2021-01-18 /pmc/articles/PMC7835979/ /pubmed/33477690 http://dx.doi.org/10.3390/pathogens10010079 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nowak, Barbara F. Dang, Mai Webber, Claire Neumann, Lukas Bridle, Andrew Bermudez, Roberto Evans, Daryl Changes in the Splenic Melanomacrophage Centre Surface Area in Southern Bluefin Tuna (Thunnus maccoyii) Are Associated with Blood Fluke Infections |
title | Changes in the Splenic Melanomacrophage Centre Surface Area in Southern Bluefin Tuna (Thunnus maccoyii) Are Associated with Blood Fluke Infections |
title_full | Changes in the Splenic Melanomacrophage Centre Surface Area in Southern Bluefin Tuna (Thunnus maccoyii) Are Associated with Blood Fluke Infections |
title_fullStr | Changes in the Splenic Melanomacrophage Centre Surface Area in Southern Bluefin Tuna (Thunnus maccoyii) Are Associated with Blood Fluke Infections |
title_full_unstemmed | Changes in the Splenic Melanomacrophage Centre Surface Area in Southern Bluefin Tuna (Thunnus maccoyii) Are Associated with Blood Fluke Infections |
title_short | Changes in the Splenic Melanomacrophage Centre Surface Area in Southern Bluefin Tuna (Thunnus maccoyii) Are Associated with Blood Fluke Infections |
title_sort | changes in the splenic melanomacrophage centre surface area in southern bluefin tuna (thunnus maccoyii) are associated with blood fluke infections |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835979/ https://www.ncbi.nlm.nih.gov/pubmed/33477690 http://dx.doi.org/10.3390/pathogens10010079 |
work_keys_str_mv | AT nowakbarbaraf changesinthesplenicmelanomacrophagecentresurfaceareainsouthernbluefintunathunnusmaccoyiiareassociatedwithbloodflukeinfections AT dangmai changesinthesplenicmelanomacrophagecentresurfaceareainsouthernbluefintunathunnusmaccoyiiareassociatedwithbloodflukeinfections AT webberclaire changesinthesplenicmelanomacrophagecentresurfaceareainsouthernbluefintunathunnusmaccoyiiareassociatedwithbloodflukeinfections AT neumannlukas changesinthesplenicmelanomacrophagecentresurfaceareainsouthernbluefintunathunnusmaccoyiiareassociatedwithbloodflukeinfections AT bridleandrew changesinthesplenicmelanomacrophagecentresurfaceareainsouthernbluefintunathunnusmaccoyiiareassociatedwithbloodflukeinfections AT bermudezroberto changesinthesplenicmelanomacrophagecentresurfaceareainsouthernbluefintunathunnusmaccoyiiareassociatedwithbloodflukeinfections AT evansdaryl changesinthesplenicmelanomacrophagecentresurfaceareainsouthernbluefintunathunnusmaccoyiiareassociatedwithbloodflukeinfections |