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Native-like SARS-CoV-2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine

Vaccine development against the SARS-CoV-2 virus focuses on the principal target of the neutralizing immune response, the spike (S) glycoprotein. Adenovirus-vectored vaccines offer an effective platform for the delivery of viral antigen, but it is important for the generation of neutralizing antibod...

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Autores principales: Watanabe, Yasunori, Mendonça, Luiza, Allen, Elizabeth R., Howe, Andrew, Lee, Mercede, Allen, Joel D., Chawla, Himanshi, Pulido, David, Donnellan, Francesca, Davies, Hannah, Ulaszewska, Marta, Belij-Rammerstorfer, Sandra, Morris, Susan, Krebs, Anna-Sophia, Dejnirattisai, Wanwisa, Mongkolsapaya, Juthathip, Supasa, Piyada, Screaton, Gavin R., Green, Catherine M., Lambe, Teresa, Zhang, Peijun, Gilbert, Sarah C., Crispin, Max
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836103/
https://www.ncbi.nlm.nih.gov/pubmed/33501433
http://dx.doi.org/10.1101/2021.01.15.426463
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author Watanabe, Yasunori
Mendonça, Luiza
Allen, Elizabeth R.
Howe, Andrew
Lee, Mercede
Allen, Joel D.
Chawla, Himanshi
Pulido, David
Donnellan, Francesca
Davies, Hannah
Ulaszewska, Marta
Belij-Rammerstorfer, Sandra
Morris, Susan
Krebs, Anna-Sophia
Dejnirattisai, Wanwisa
Mongkolsapaya, Juthathip
Supasa, Piyada
Screaton, Gavin R.
Green, Catherine M.
Lambe, Teresa
Zhang, Peijun
Gilbert, Sarah C.
Crispin, Max
author_facet Watanabe, Yasunori
Mendonça, Luiza
Allen, Elizabeth R.
Howe, Andrew
Lee, Mercede
Allen, Joel D.
Chawla, Himanshi
Pulido, David
Donnellan, Francesca
Davies, Hannah
Ulaszewska, Marta
Belij-Rammerstorfer, Sandra
Morris, Susan
Krebs, Anna-Sophia
Dejnirattisai, Wanwisa
Mongkolsapaya, Juthathip
Supasa, Piyada
Screaton, Gavin R.
Green, Catherine M.
Lambe, Teresa
Zhang, Peijun
Gilbert, Sarah C.
Crispin, Max
author_sort Watanabe, Yasunori
collection PubMed
description Vaccine development against the SARS-CoV-2 virus focuses on the principal target of the neutralizing immune response, the spike (S) glycoprotein. Adenovirus-vectored vaccines offer an effective platform for the delivery of viral antigen, but it is important for the generation of neutralizing antibodies that they produce appropriately processed and assembled viral antigen that mimics that observed on the SARS-CoV-2 virus. Here, we describe the structure, conformation and glycosylation of the S protein derived from the adenovirus-vectored ChAdOx1 nCoV-19/AZD1222 vaccine. We demonstrate native-like post-translational processing and assembly, and reveal the expression of S proteins on the surface of cells adopting the trimeric prefusion conformation. The data presented here confirms the use of ChAdOx1 adenovirus vectors as a leading platform technology for SARS-CoV-2 vaccines.
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spelling pubmed-78361032021-01-27 Native-like SARS-CoV-2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine Watanabe, Yasunori Mendonça, Luiza Allen, Elizabeth R. Howe, Andrew Lee, Mercede Allen, Joel D. Chawla, Himanshi Pulido, David Donnellan, Francesca Davies, Hannah Ulaszewska, Marta Belij-Rammerstorfer, Sandra Morris, Susan Krebs, Anna-Sophia Dejnirattisai, Wanwisa Mongkolsapaya, Juthathip Supasa, Piyada Screaton, Gavin R. Green, Catherine M. Lambe, Teresa Zhang, Peijun Gilbert, Sarah C. Crispin, Max bioRxiv Article Vaccine development against the SARS-CoV-2 virus focuses on the principal target of the neutralizing immune response, the spike (S) glycoprotein. Adenovirus-vectored vaccines offer an effective platform for the delivery of viral antigen, but it is important for the generation of neutralizing antibodies that they produce appropriately processed and assembled viral antigen that mimics that observed on the SARS-CoV-2 virus. Here, we describe the structure, conformation and glycosylation of the S protein derived from the adenovirus-vectored ChAdOx1 nCoV-19/AZD1222 vaccine. We demonstrate native-like post-translational processing and assembly, and reveal the expression of S proteins on the surface of cells adopting the trimeric prefusion conformation. The data presented here confirms the use of ChAdOx1 adenovirus vectors as a leading platform technology for SARS-CoV-2 vaccines. Cold Spring Harbor Laboratory 2021-01-19 /pmc/articles/PMC7836103/ /pubmed/33501433 http://dx.doi.org/10.1101/2021.01.15.426463 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Watanabe, Yasunori
Mendonça, Luiza
Allen, Elizabeth R.
Howe, Andrew
Lee, Mercede
Allen, Joel D.
Chawla, Himanshi
Pulido, David
Donnellan, Francesca
Davies, Hannah
Ulaszewska, Marta
Belij-Rammerstorfer, Sandra
Morris, Susan
Krebs, Anna-Sophia
Dejnirattisai, Wanwisa
Mongkolsapaya, Juthathip
Supasa, Piyada
Screaton, Gavin R.
Green, Catherine M.
Lambe, Teresa
Zhang, Peijun
Gilbert, Sarah C.
Crispin, Max
Native-like SARS-CoV-2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine
title Native-like SARS-CoV-2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine
title_full Native-like SARS-CoV-2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine
title_fullStr Native-like SARS-CoV-2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine
title_full_unstemmed Native-like SARS-CoV-2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine
title_short Native-like SARS-CoV-2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine
title_sort native-like sars-cov-2 spike glycoprotein expressed by chadox1 ncov-19/azd1222 vaccine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836103/
https://www.ncbi.nlm.nih.gov/pubmed/33501433
http://dx.doi.org/10.1101/2021.01.15.426463
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