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Correlative Multi-scale Cryo-imaging Unveils SARS-CoV-2 Assembly and Egress
Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified recombinant viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the frozen-hydrated native cellular context is sc...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Journal Experts
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836121/ https://www.ncbi.nlm.nih.gov/pubmed/33501431 http://dx.doi.org/10.21203/rs.3.rs-134794/v1 |
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author | Zhang, Peijun Mendonca, Luiza Howe, Andrew Gilchrist, James Sun, Dapeng Knight, Michael Zanetti-Domingues, Laura Bateman, Benji Krebs, Anna-Sophia Chen, Long Radecke, Julika Sheng, Yuewen Li, Vivian Ni, Tao Kounatidis, Ilias Koronfel, Mohamed Szynkiewicz, Marta Harkiolaki, Maria Martin-Fernandez, Marisa James, William |
author_facet | Zhang, Peijun Mendonca, Luiza Howe, Andrew Gilchrist, James Sun, Dapeng Knight, Michael Zanetti-Domingues, Laura Bateman, Benji Krebs, Anna-Sophia Chen, Long Radecke, Julika Sheng, Yuewen Li, Vivian Ni, Tao Kounatidis, Ilias Koronfel, Mohamed Szynkiewicz, Marta Harkiolaki, Maria Martin-Fernandez, Marisa James, William |
author_sort | Zhang, Peijun |
collection | PubMed |
description | Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified recombinant viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the frozen-hydrated native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate the cytopathic events induced by SARS-CoV-2 with virus replication process under the frozen-hydrated condition, here we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. The results place critical SARS-CoV-2 structural events – e.g. viral RNA transport portals on double membrane vesicles, virus assembly and budding intermediates, virus egress pathways, and native virus spike structures from intracellular assembled and extracellular released virus - in the context of whole-cell images. The latter revealed numerous heterogeneous cytoplasmic vesicles, the formation of membrane tunnels through which viruses exit, and the drastic cytoplasm invasion into the nucleus. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules. |
format | Online Article Text |
id | pubmed-7836121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Journal Experts |
record_format | MEDLINE/PubMed |
spelling | pubmed-78361212021-01-27 Correlative Multi-scale Cryo-imaging Unveils SARS-CoV-2 Assembly and Egress Zhang, Peijun Mendonca, Luiza Howe, Andrew Gilchrist, James Sun, Dapeng Knight, Michael Zanetti-Domingues, Laura Bateman, Benji Krebs, Anna-Sophia Chen, Long Radecke, Julika Sheng, Yuewen Li, Vivian Ni, Tao Kounatidis, Ilias Koronfel, Mohamed Szynkiewicz, Marta Harkiolaki, Maria Martin-Fernandez, Marisa James, William Res Sq Article Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified recombinant viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the frozen-hydrated native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate the cytopathic events induced by SARS-CoV-2 with virus replication process under the frozen-hydrated condition, here we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. The results place critical SARS-CoV-2 structural events – e.g. viral RNA transport portals on double membrane vesicles, virus assembly and budding intermediates, virus egress pathways, and native virus spike structures from intracellular assembled and extracellular released virus - in the context of whole-cell images. The latter revealed numerous heterogeneous cytoplasmic vesicles, the formation of membrane tunnels through which viruses exit, and the drastic cytoplasm invasion into the nucleus. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules. American Journal Experts 2021-01-19 /pmc/articles/PMC7836121/ /pubmed/33501431 http://dx.doi.org/10.21203/rs.3.rs-134794/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Article Zhang, Peijun Mendonca, Luiza Howe, Andrew Gilchrist, James Sun, Dapeng Knight, Michael Zanetti-Domingues, Laura Bateman, Benji Krebs, Anna-Sophia Chen, Long Radecke, Julika Sheng, Yuewen Li, Vivian Ni, Tao Kounatidis, Ilias Koronfel, Mohamed Szynkiewicz, Marta Harkiolaki, Maria Martin-Fernandez, Marisa James, William Correlative Multi-scale Cryo-imaging Unveils SARS-CoV-2 Assembly and Egress |
title | Correlative Multi-scale Cryo-imaging Unveils SARS-CoV-2 Assembly and Egress |
title_full | Correlative Multi-scale Cryo-imaging Unveils SARS-CoV-2 Assembly and Egress |
title_fullStr | Correlative Multi-scale Cryo-imaging Unveils SARS-CoV-2 Assembly and Egress |
title_full_unstemmed | Correlative Multi-scale Cryo-imaging Unveils SARS-CoV-2 Assembly and Egress |
title_short | Correlative Multi-scale Cryo-imaging Unveils SARS-CoV-2 Assembly and Egress |
title_sort | correlative multi-scale cryo-imaging unveils sars-cov-2 assembly and egress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836121/ https://www.ncbi.nlm.nih.gov/pubmed/33501431 http://dx.doi.org/10.21203/rs.3.rs-134794/v1 |
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