Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimens

We evaluated sweat, blood and urine specimens obtained from an ongoing cohort study in Brazil. Samples were collected at pre-established intervals after the initial rash presentation and tested for Zika virus (ZIKV) RNA presence by real-time reverse transcriptase polymerase chain reaction (rRT-PCR)....

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Detalles Bibliográficos
Autores principales: Menezes-Neto, Armando, Castilho, Marcia da Costa, Calvet, Guilherme Amaral, Kara, Edna Oliveira, Bôtto-Menezes, Camila Helena Aguiar, Lacerda, Marcus Vinícius Guimarães, Pereira, Gerson Fernando Mendes, Giozza, Silvana Pereira, Bermudez, Ximena Pamela Diaz, Lima, Noemia Santana, Modjarrad, Kayvon, Broutet, Nathalie, de Filippis, Ana Maria Bispo, Franca, Rafael Freitas Oliveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836980/
https://www.ncbi.nlm.nih.gov/pubmed/33503145
http://dx.doi.org/10.1590/0074-02760200339
Descripción
Sumario:We evaluated sweat, blood and urine specimens obtained from an ongoing cohort study in Brazil. Samples were collected at pre-established intervals after the initial rash presentation and tested for Zika virus (ZIKV) RNA presence by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). From 254 participants with confirmed infection, ZIKV RNA was detected in the sweat of 46 individuals (18.1%). Sweat presented a median cycle threshold (Ct) of 34.74 [interquartile range (IQR) 33.44-36.04], comparable to plasma (Ct 35.96 - IQR 33.29-36.69) and higher than urine (Ct 30.78 - IQR 28.72-33.22). Concomitant detection with other specimens was observed in 33 (72%) of 46 participants who had a positive result in sweat. These findings represent an unusual and not yet investigated virus shedding through eccrine glands.

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