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Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research

Several commercial Zika virus (ZIKV) serology assays have been developed since the recognition of ZIKV outbreaks as a Public Health Emergency of International Concern in 2016. However, test interpretation for ZIKV serology can be challenging due to antibody cross-reactivity with other flaviviruses l...

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Autores principales: Low, Swee Ling, Leo, Yee Sin, Lai, Yee Ling, Lam, Sally, Tan, Hwee Huang, Wong, Judith Chui Ching, Tan, Li Kiang, Ng, Lee Ching
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837473/
https://www.ncbi.nlm.nih.gov/pubmed/33497414
http://dx.doi.org/10.1371/journal.pone.0244601
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author Low, Swee Ling
Leo, Yee Sin
Lai, Yee Ling
Lam, Sally
Tan, Hwee Huang
Wong, Judith Chui Ching
Tan, Li Kiang
Ng, Lee Ching
author_facet Low, Swee Ling
Leo, Yee Sin
Lai, Yee Ling
Lam, Sally
Tan, Hwee Huang
Wong, Judith Chui Ching
Tan, Li Kiang
Ng, Lee Ching
author_sort Low, Swee Ling
collection PubMed
description Several commercial Zika virus (ZIKV) serology assays have been developed since the recognition of ZIKV outbreaks as a Public Health Emergency of International Concern in 2016. However, test interpretation for ZIKV serology can be challenging due to antibody cross-reactivity with other flaviviruses like dengue virus (DENV). Therefore, we sought to evaluate the performance of eight commercially available ZIKV IgM and IgG assays across three testing platforms, namely, immunochromatographic tests (ICT), ELISAs and immunofluorescence tests (IIFT). The test panel comprised of 278 samples, including acute and convalescent sera or plasma from ZIKV-confirmed, DENV-confirmed, non-ZIKV and non-DENV patients, and residual sera from healthy blood donors. The ZIKV IgM and IgG serology assays yielded higher test sensitivities of 23.5% - 97.1% among ZIKV convalescent samples as compared to 5.6% - 27.8% among ZIKV acute samples; the test specificities were 63.3% - 100% among acute and convalescent DENV, non-DENV samples. Among the ELISAs and IIFTs, the Diapro ZIKV IgM ELISA demonstrated high test sensitivity (96%) and specificity (80%) when tested on early convalescent samples, while the Euroimmun ZIKV IgG ELISA yielded the highest test specificity of 97% - 100% on samples from non-ZIKV patients and healthy blood donors. For rapid ICTs, the LumiQuick IgM rapid ICT yielded low test sensitivity, suggesting its limited utility. We showed that commercial ZIKV IgM and IgG serology assays have differing test performances, with some having moderate to high test sensitivities and specificities when used in a dengue endemic setting, although there were limitations in IgG serology.
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spelling pubmed-78374732021-02-02 Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research Low, Swee Ling Leo, Yee Sin Lai, Yee Ling Lam, Sally Tan, Hwee Huang Wong, Judith Chui Ching Tan, Li Kiang Ng, Lee Ching PLoS One Research Article Several commercial Zika virus (ZIKV) serology assays have been developed since the recognition of ZIKV outbreaks as a Public Health Emergency of International Concern in 2016. However, test interpretation for ZIKV serology can be challenging due to antibody cross-reactivity with other flaviviruses like dengue virus (DENV). Therefore, we sought to evaluate the performance of eight commercially available ZIKV IgM and IgG assays across three testing platforms, namely, immunochromatographic tests (ICT), ELISAs and immunofluorescence tests (IIFT). The test panel comprised of 278 samples, including acute and convalescent sera or plasma from ZIKV-confirmed, DENV-confirmed, non-ZIKV and non-DENV patients, and residual sera from healthy blood donors. The ZIKV IgM and IgG serology assays yielded higher test sensitivities of 23.5% - 97.1% among ZIKV convalescent samples as compared to 5.6% - 27.8% among ZIKV acute samples; the test specificities were 63.3% - 100% among acute and convalescent DENV, non-DENV samples. Among the ELISAs and IIFTs, the Diapro ZIKV IgM ELISA demonstrated high test sensitivity (96%) and specificity (80%) when tested on early convalescent samples, while the Euroimmun ZIKV IgG ELISA yielded the highest test specificity of 97% - 100% on samples from non-ZIKV patients and healthy blood donors. For rapid ICTs, the LumiQuick IgM rapid ICT yielded low test sensitivity, suggesting its limited utility. We showed that commercial ZIKV IgM and IgG serology assays have differing test performances, with some having moderate to high test sensitivities and specificities when used in a dengue endemic setting, although there were limitations in IgG serology. Public Library of Science 2021-01-26 /pmc/articles/PMC7837473/ /pubmed/33497414 http://dx.doi.org/10.1371/journal.pone.0244601 Text en © 2021 Low et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Low, Swee Ling
Leo, Yee Sin
Lai, Yee Ling
Lam, Sally
Tan, Hwee Huang
Wong, Judith Chui Ching
Tan, Li Kiang
Ng, Lee Ching
Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research
title Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research
title_full Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research
title_fullStr Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research
title_full_unstemmed Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research
title_short Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research
title_sort evaluation of eight commercial zika virus igm and igg serology assays for diagnostics and research
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837473/
https://www.ncbi.nlm.nih.gov/pubmed/33497414
http://dx.doi.org/10.1371/journal.pone.0244601
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