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Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research
Several commercial Zika virus (ZIKV) serology assays have been developed since the recognition of ZIKV outbreaks as a Public Health Emergency of International Concern in 2016. However, test interpretation for ZIKV serology can be challenging due to antibody cross-reactivity with other flaviviruses l...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837473/ https://www.ncbi.nlm.nih.gov/pubmed/33497414 http://dx.doi.org/10.1371/journal.pone.0244601 |
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author | Low, Swee Ling Leo, Yee Sin Lai, Yee Ling Lam, Sally Tan, Hwee Huang Wong, Judith Chui Ching Tan, Li Kiang Ng, Lee Ching |
author_facet | Low, Swee Ling Leo, Yee Sin Lai, Yee Ling Lam, Sally Tan, Hwee Huang Wong, Judith Chui Ching Tan, Li Kiang Ng, Lee Ching |
author_sort | Low, Swee Ling |
collection | PubMed |
description | Several commercial Zika virus (ZIKV) serology assays have been developed since the recognition of ZIKV outbreaks as a Public Health Emergency of International Concern in 2016. However, test interpretation for ZIKV serology can be challenging due to antibody cross-reactivity with other flaviviruses like dengue virus (DENV). Therefore, we sought to evaluate the performance of eight commercially available ZIKV IgM and IgG assays across three testing platforms, namely, immunochromatographic tests (ICT), ELISAs and immunofluorescence tests (IIFT). The test panel comprised of 278 samples, including acute and convalescent sera or plasma from ZIKV-confirmed, DENV-confirmed, non-ZIKV and non-DENV patients, and residual sera from healthy blood donors. The ZIKV IgM and IgG serology assays yielded higher test sensitivities of 23.5% - 97.1% among ZIKV convalescent samples as compared to 5.6% - 27.8% among ZIKV acute samples; the test specificities were 63.3% - 100% among acute and convalescent DENV, non-DENV samples. Among the ELISAs and IIFTs, the Diapro ZIKV IgM ELISA demonstrated high test sensitivity (96%) and specificity (80%) when tested on early convalescent samples, while the Euroimmun ZIKV IgG ELISA yielded the highest test specificity of 97% - 100% on samples from non-ZIKV patients and healthy blood donors. For rapid ICTs, the LumiQuick IgM rapid ICT yielded low test sensitivity, suggesting its limited utility. We showed that commercial ZIKV IgM and IgG serology assays have differing test performances, with some having moderate to high test sensitivities and specificities when used in a dengue endemic setting, although there were limitations in IgG serology. |
format | Online Article Text |
id | pubmed-7837473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78374732021-02-02 Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research Low, Swee Ling Leo, Yee Sin Lai, Yee Ling Lam, Sally Tan, Hwee Huang Wong, Judith Chui Ching Tan, Li Kiang Ng, Lee Ching PLoS One Research Article Several commercial Zika virus (ZIKV) serology assays have been developed since the recognition of ZIKV outbreaks as a Public Health Emergency of International Concern in 2016. However, test interpretation for ZIKV serology can be challenging due to antibody cross-reactivity with other flaviviruses like dengue virus (DENV). Therefore, we sought to evaluate the performance of eight commercially available ZIKV IgM and IgG assays across three testing platforms, namely, immunochromatographic tests (ICT), ELISAs and immunofluorescence tests (IIFT). The test panel comprised of 278 samples, including acute and convalescent sera or plasma from ZIKV-confirmed, DENV-confirmed, non-ZIKV and non-DENV patients, and residual sera from healthy blood donors. The ZIKV IgM and IgG serology assays yielded higher test sensitivities of 23.5% - 97.1% among ZIKV convalescent samples as compared to 5.6% - 27.8% among ZIKV acute samples; the test specificities were 63.3% - 100% among acute and convalescent DENV, non-DENV samples. Among the ELISAs and IIFTs, the Diapro ZIKV IgM ELISA demonstrated high test sensitivity (96%) and specificity (80%) when tested on early convalescent samples, while the Euroimmun ZIKV IgG ELISA yielded the highest test specificity of 97% - 100% on samples from non-ZIKV patients and healthy blood donors. For rapid ICTs, the LumiQuick IgM rapid ICT yielded low test sensitivity, suggesting its limited utility. We showed that commercial ZIKV IgM and IgG serology assays have differing test performances, with some having moderate to high test sensitivities and specificities when used in a dengue endemic setting, although there were limitations in IgG serology. Public Library of Science 2021-01-26 /pmc/articles/PMC7837473/ /pubmed/33497414 http://dx.doi.org/10.1371/journal.pone.0244601 Text en © 2021 Low et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Low, Swee Ling Leo, Yee Sin Lai, Yee Ling Lam, Sally Tan, Hwee Huang Wong, Judith Chui Ching Tan, Li Kiang Ng, Lee Ching Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research |
title | Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research |
title_full | Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research |
title_fullStr | Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research |
title_full_unstemmed | Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research |
title_short | Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research |
title_sort | evaluation of eight commercial zika virus igm and igg serology assays for diagnostics and research |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837473/ https://www.ncbi.nlm.nih.gov/pubmed/33497414 http://dx.doi.org/10.1371/journal.pone.0244601 |
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