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Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection
In this study transcriptome was analyzed on two fibrous varieties of flax: the susceptible Regina and the resistant Nike. The experiment was carried out on 2-week-old seedlings, because in this phase of development flax is the most susceptible to infection. We analyzed the whole seedlings, which all...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837494/ https://www.ncbi.nlm.nih.gov/pubmed/33497403 http://dx.doi.org/10.1371/journal.pone.0246052 |
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author | Boba, Aleksandra Kostyn, Kamil Kozak, Bartosz Zalewski, Iwan Szopa, Jan Kulma, Anna |
author_facet | Boba, Aleksandra Kostyn, Kamil Kozak, Bartosz Zalewski, Iwan Szopa, Jan Kulma, Anna |
author_sort | Boba, Aleksandra |
collection | PubMed |
description | In this study transcriptome was analyzed on two fibrous varieties of flax: the susceptible Regina and the resistant Nike. The experiment was carried out on 2-week-old seedlings, because in this phase of development flax is the most susceptible to infection. We analyzed the whole seedlings, which allowed us to recognize the systemic response of the plants to the infection. We decided to analyze two time points: 24h and 48h, because our goal was to learn the mechanisms activated in the initial stages of infection, these points were selected based on the previous analysis of chitinase gene expression, whose increase in time of Fusarium oxysporum lini infection has been repeatedly confirmed both in the case of flax and other plant species. The results show that although qualitatively the responses of the two varieties are similar, it is the degree of the response that plays the role in the differences of their resistance to F. oxysporum. |
format | Online Article Text |
id | pubmed-7837494 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78374942021-02-02 Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection Boba, Aleksandra Kostyn, Kamil Kozak, Bartosz Zalewski, Iwan Szopa, Jan Kulma, Anna PLoS One Research Article In this study transcriptome was analyzed on two fibrous varieties of flax: the susceptible Regina and the resistant Nike. The experiment was carried out on 2-week-old seedlings, because in this phase of development flax is the most susceptible to infection. We analyzed the whole seedlings, which allowed us to recognize the systemic response of the plants to the infection. We decided to analyze two time points: 24h and 48h, because our goal was to learn the mechanisms activated in the initial stages of infection, these points were selected based on the previous analysis of chitinase gene expression, whose increase in time of Fusarium oxysporum lini infection has been repeatedly confirmed both in the case of flax and other plant species. The results show that although qualitatively the responses of the two varieties are similar, it is the degree of the response that plays the role in the differences of their resistance to F. oxysporum. Public Library of Science 2021-01-26 /pmc/articles/PMC7837494/ /pubmed/33497403 http://dx.doi.org/10.1371/journal.pone.0246052 Text en © 2021 Boba et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Boba, Aleksandra Kostyn, Kamil Kozak, Bartosz Zalewski, Iwan Szopa, Jan Kulma, Anna Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection |
title | Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection |
title_full | Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection |
title_fullStr | Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection |
title_full_unstemmed | Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection |
title_short | Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection |
title_sort | transcriptomic profiling of susceptible and resistant flax seedlings after fusarium oxysporum lini infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837494/ https://www.ncbi.nlm.nih.gov/pubmed/33497403 http://dx.doi.org/10.1371/journal.pone.0246052 |
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