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Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection

In this study transcriptome was analyzed on two fibrous varieties of flax: the susceptible Regina and the resistant Nike. The experiment was carried out on 2-week-old seedlings, because in this phase of development flax is the most susceptible to infection. We analyzed the whole seedlings, which all...

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Autores principales: Boba, Aleksandra, Kostyn, Kamil, Kozak, Bartosz, Zalewski, Iwan, Szopa, Jan, Kulma, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837494/
https://www.ncbi.nlm.nih.gov/pubmed/33497403
http://dx.doi.org/10.1371/journal.pone.0246052
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author Boba, Aleksandra
Kostyn, Kamil
Kozak, Bartosz
Zalewski, Iwan
Szopa, Jan
Kulma, Anna
author_facet Boba, Aleksandra
Kostyn, Kamil
Kozak, Bartosz
Zalewski, Iwan
Szopa, Jan
Kulma, Anna
author_sort Boba, Aleksandra
collection PubMed
description In this study transcriptome was analyzed on two fibrous varieties of flax: the susceptible Regina and the resistant Nike. The experiment was carried out on 2-week-old seedlings, because in this phase of development flax is the most susceptible to infection. We analyzed the whole seedlings, which allowed us to recognize the systemic response of the plants to the infection. We decided to analyze two time points: 24h and 48h, because our goal was to learn the mechanisms activated in the initial stages of infection, these points were selected based on the previous analysis of chitinase gene expression, whose increase in time of Fusarium oxysporum lini infection has been repeatedly confirmed both in the case of flax and other plant species. The results show that although qualitatively the responses of the two varieties are similar, it is the degree of the response that plays the role in the differences of their resistance to F. oxysporum.
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spelling pubmed-78374942021-02-02 Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection Boba, Aleksandra Kostyn, Kamil Kozak, Bartosz Zalewski, Iwan Szopa, Jan Kulma, Anna PLoS One Research Article In this study transcriptome was analyzed on two fibrous varieties of flax: the susceptible Regina and the resistant Nike. The experiment was carried out on 2-week-old seedlings, because in this phase of development flax is the most susceptible to infection. We analyzed the whole seedlings, which allowed us to recognize the systemic response of the plants to the infection. We decided to analyze two time points: 24h and 48h, because our goal was to learn the mechanisms activated in the initial stages of infection, these points were selected based on the previous analysis of chitinase gene expression, whose increase in time of Fusarium oxysporum lini infection has been repeatedly confirmed both in the case of flax and other plant species. The results show that although qualitatively the responses of the two varieties are similar, it is the degree of the response that plays the role in the differences of their resistance to F. oxysporum. Public Library of Science 2021-01-26 /pmc/articles/PMC7837494/ /pubmed/33497403 http://dx.doi.org/10.1371/journal.pone.0246052 Text en © 2021 Boba et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Boba, Aleksandra
Kostyn, Kamil
Kozak, Bartosz
Zalewski, Iwan
Szopa, Jan
Kulma, Anna
Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection
title Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection
title_full Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection
title_fullStr Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection
title_full_unstemmed Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection
title_short Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection
title_sort transcriptomic profiling of susceptible and resistant flax seedlings after fusarium oxysporum lini infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837494/
https://www.ncbi.nlm.nih.gov/pubmed/33497403
http://dx.doi.org/10.1371/journal.pone.0246052
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