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Collateral sensitivity associated with antibiotic resistance plasmids

Collateral sensitivity (CS) is a promising alternative approach to counteract the rising problem of antibiotic resistance (ABR). CS occurs when the acquisition of resistance to one antibiotic produces increased susceptibility to a second antibiotic. Recent studies have focused on CS strategies desig...

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Autores principales: Herencias, Cristina, Rodríguez-Beltrán, Jerónimo, León-Sampedro, Ricardo, Alonso-del Valle, Aida, Palkovičová, Jana, Cantón, Rafael, San Millán, Álvaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837676/
https://www.ncbi.nlm.nih.gov/pubmed/33470194
http://dx.doi.org/10.7554/eLife.65130
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author Herencias, Cristina
Rodríguez-Beltrán, Jerónimo
León-Sampedro, Ricardo
Alonso-del Valle, Aida
Palkovičová, Jana
Cantón, Rafael
San Millán, Álvaro
author_facet Herencias, Cristina
Rodríguez-Beltrán, Jerónimo
León-Sampedro, Ricardo
Alonso-del Valle, Aida
Palkovičová, Jana
Cantón, Rafael
San Millán, Álvaro
author_sort Herencias, Cristina
collection PubMed
description Collateral sensitivity (CS) is a promising alternative approach to counteract the rising problem of antibiotic resistance (ABR). CS occurs when the acquisition of resistance to one antibiotic produces increased susceptibility to a second antibiotic. Recent studies have focused on CS strategies designed against ABR mediated by chromosomal mutations. However, one of the main drivers of ABR in clinically relevant bacteria is the horizontal transfer of ABR genes mediated by plasmids. Here, we report the first analysis of CS associated with the acquisition of complete ABR plasmids, including the clinically important carbapenem-resistance conjugative plasmid pOXA-48. In addition, we describe the conservation of CS in clinical E. coli isolates and its application to selectively kill plasmid-carrying bacteria. Our results provide new insights that establish the basis for developing CS-informed treatment strategies to combat plasmid-mediated ABR.
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spelling pubmed-78376762021-01-27 Collateral sensitivity associated with antibiotic resistance plasmids Herencias, Cristina Rodríguez-Beltrán, Jerónimo León-Sampedro, Ricardo Alonso-del Valle, Aida Palkovičová, Jana Cantón, Rafael San Millán, Álvaro eLife Evolutionary Biology Collateral sensitivity (CS) is a promising alternative approach to counteract the rising problem of antibiotic resistance (ABR). CS occurs when the acquisition of resistance to one antibiotic produces increased susceptibility to a second antibiotic. Recent studies have focused on CS strategies designed against ABR mediated by chromosomal mutations. However, one of the main drivers of ABR in clinically relevant bacteria is the horizontal transfer of ABR genes mediated by plasmids. Here, we report the first analysis of CS associated with the acquisition of complete ABR plasmids, including the clinically important carbapenem-resistance conjugative plasmid pOXA-48. In addition, we describe the conservation of CS in clinical E. coli isolates and its application to selectively kill plasmid-carrying bacteria. Our results provide new insights that establish the basis for developing CS-informed treatment strategies to combat plasmid-mediated ABR. eLife Sciences Publications, Ltd 2021-01-20 /pmc/articles/PMC7837676/ /pubmed/33470194 http://dx.doi.org/10.7554/eLife.65130 Text en © 2021, Herencias et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Evolutionary Biology
Herencias, Cristina
Rodríguez-Beltrán, Jerónimo
León-Sampedro, Ricardo
Alonso-del Valle, Aida
Palkovičová, Jana
Cantón, Rafael
San Millán, Álvaro
Collateral sensitivity associated with antibiotic resistance plasmids
title Collateral sensitivity associated with antibiotic resistance plasmids
title_full Collateral sensitivity associated with antibiotic resistance plasmids
title_fullStr Collateral sensitivity associated with antibiotic resistance plasmids
title_full_unstemmed Collateral sensitivity associated with antibiotic resistance plasmids
title_short Collateral sensitivity associated with antibiotic resistance plasmids
title_sort collateral sensitivity associated with antibiotic resistance plasmids
topic Evolutionary Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837676/
https://www.ncbi.nlm.nih.gov/pubmed/33470194
http://dx.doi.org/10.7554/eLife.65130
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