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A cell atlas of the chick retina based on single-cell transcriptomics
Retinal structure and function have been studied in many vertebrate orders, but molecular characterization has been largely confined to mammals. We used single-cell RNA sequencing (scRNA-seq) to generate a cell atlas of the chick retina. We identified 136 cell types plus 14 positional or development...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837701/ https://www.ncbi.nlm.nih.gov/pubmed/33393903 http://dx.doi.org/10.7554/eLife.63907 |
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author | Yamagata, Masahito Yan, Wenjun Sanes, Joshua R |
author_facet | Yamagata, Masahito Yan, Wenjun Sanes, Joshua R |
author_sort | Yamagata, Masahito |
collection | PubMed |
description | Retinal structure and function have been studied in many vertebrate orders, but molecular characterization has been largely confined to mammals. We used single-cell RNA sequencing (scRNA-seq) to generate a cell atlas of the chick retina. We identified 136 cell types plus 14 positional or developmental intermediates distributed among the six classes conserved across vertebrates – photoreceptor, horizontal, bipolar, amacrine, retinal ganglion, and glial cells. To assess morphology of molecularly defined types, we adapted a method for CRISPR-based integration of reporters into selectively expressed genes. For Müller glia, we found that transcriptionally distinct cells were regionally localized along the anterior-posterior, dorsal-ventral, and central-peripheral retinal axes. We also identified immature photoreceptor, horizontal cell, and oligodendrocyte types that persist into late embryonic stages. Finally, we analyzed relationships among chick, mouse, and primate retinal cell classes and types. Our results provide a foundation for anatomical, physiological, evolutionary, and developmental studies of the avian visual system. |
format | Online Article Text |
id | pubmed-7837701 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-78377012021-01-27 A cell atlas of the chick retina based on single-cell transcriptomics Yamagata, Masahito Yan, Wenjun Sanes, Joshua R eLife Neuroscience Retinal structure and function have been studied in many vertebrate orders, but molecular characterization has been largely confined to mammals. We used single-cell RNA sequencing (scRNA-seq) to generate a cell atlas of the chick retina. We identified 136 cell types plus 14 positional or developmental intermediates distributed among the six classes conserved across vertebrates – photoreceptor, horizontal, bipolar, amacrine, retinal ganglion, and glial cells. To assess morphology of molecularly defined types, we adapted a method for CRISPR-based integration of reporters into selectively expressed genes. For Müller glia, we found that transcriptionally distinct cells were regionally localized along the anterior-posterior, dorsal-ventral, and central-peripheral retinal axes. We also identified immature photoreceptor, horizontal cell, and oligodendrocyte types that persist into late embryonic stages. Finally, we analyzed relationships among chick, mouse, and primate retinal cell classes and types. Our results provide a foundation for anatomical, physiological, evolutionary, and developmental studies of the avian visual system. eLife Sciences Publications, Ltd 2021-01-04 /pmc/articles/PMC7837701/ /pubmed/33393903 http://dx.doi.org/10.7554/eLife.63907 Text en © 2021, Yamagata et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Yamagata, Masahito Yan, Wenjun Sanes, Joshua R A cell atlas of the chick retina based on single-cell transcriptomics |
title | A cell atlas of the chick retina based on single-cell transcriptomics |
title_full | A cell atlas of the chick retina based on single-cell transcriptomics |
title_fullStr | A cell atlas of the chick retina based on single-cell transcriptomics |
title_full_unstemmed | A cell atlas of the chick retina based on single-cell transcriptomics |
title_short | A cell atlas of the chick retina based on single-cell transcriptomics |
title_sort | cell atlas of the chick retina based on single-cell transcriptomics |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837701/ https://www.ncbi.nlm.nih.gov/pubmed/33393903 http://dx.doi.org/10.7554/eLife.63907 |
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