Cargando…

Ex Ovo Culture System for Avian Embryos and its Application

Ex ovo culture of avian embryos can be applied not only to embryology but also to various fields of basic research such as embryo manipulation, toxicology, and regenerative medicine. The windowing method, which facilitates various manipulations and observations by opening a hole in one part of the e...

Descripción completa

Detalles Bibliográficos
Autores principales: Tahara, Yutaka, Obara, Katsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japan Poultry Science Association 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837809/
https://www.ncbi.nlm.nih.gov/pubmed/33519280
http://dx.doi.org/10.2141/jpsa.0200016
_version_ 1783643027932184576
author Tahara, Yutaka
Obara, Katsuya
author_facet Tahara, Yutaka
Obara, Katsuya
author_sort Tahara, Yutaka
collection PubMed
description Ex ovo culture of avian embryos can be applied not only to embryology but also to various fields of basic research such as embryo manipulation, toxicology, and regenerative medicine. The windowing method, which facilitates various manipulations and observations by opening a hole in one part of the eggshell, and culture systems using surrogate eggshells, are widely used. Despite this, biology lessons in high schools cover shell-less culture systems, which involve the development of avian embryos in artificial vessels, such as rice bowls, without using surrogate eggshells. However, as embryo development stops at its early stages in this method, it is not possible to continuously observe the development of the embryo. This led to attempts to develop an embryo culture method using a complete artificial culture vessel that does not use surrogate eggshells, and Kamihira et al. (1998) succeeded in hatching quail embryos in an artificial culture vessel using polytetrafluoroethylene membranes. In addition, Tahara succeeded in hatching chick embryos in artificial culture vessels that used cling film made of polymethylpentene and reported their detailed methodology (Tahara and Obara, 2014). These technologies are being applied not only to school education but also to various fields of research.
format Online
Article
Text
id pubmed-7837809
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Japan Poultry Science Association
record_format MEDLINE/PubMed
spelling pubmed-78378092021-01-28 Ex Ovo Culture System for Avian Embryos and its Application Tahara, Yutaka Obara, Katsuya J Poult Sci Review Ex ovo culture of avian embryos can be applied not only to embryology but also to various fields of basic research such as embryo manipulation, toxicology, and regenerative medicine. The windowing method, which facilitates various manipulations and observations by opening a hole in one part of the eggshell, and culture systems using surrogate eggshells, are widely used. Despite this, biology lessons in high schools cover shell-less culture systems, which involve the development of avian embryos in artificial vessels, such as rice bowls, without using surrogate eggshells. However, as embryo development stops at its early stages in this method, it is not possible to continuously observe the development of the embryo. This led to attempts to develop an embryo culture method using a complete artificial culture vessel that does not use surrogate eggshells, and Kamihira et al. (1998) succeeded in hatching quail embryos in an artificial culture vessel using polytetrafluoroethylene membranes. In addition, Tahara succeeded in hatching chick embryos in artificial culture vessels that used cling film made of polymethylpentene and reported their detailed methodology (Tahara and Obara, 2014). These technologies are being applied not only to school education but also to various fields of research. Japan Poultry Science Association 2021-01-25 /pmc/articles/PMC7837809/ /pubmed/33519280 http://dx.doi.org/10.2141/jpsa.0200016 Text en 2020, Japan Poultry Science Association. The Journal of Poultry Science is an Open Access journal distributed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. To view the details of this license, please visit (https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Review
Tahara, Yutaka
Obara, Katsuya
Ex Ovo Culture System for Avian Embryos and its Application
title Ex Ovo Culture System for Avian Embryos and its Application
title_full Ex Ovo Culture System for Avian Embryos and its Application
title_fullStr Ex Ovo Culture System for Avian Embryos and its Application
title_full_unstemmed Ex Ovo Culture System for Avian Embryos and its Application
title_short Ex Ovo Culture System for Avian Embryos and its Application
title_sort ex ovo culture system for avian embryos and its application
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837809/
https://www.ncbi.nlm.nih.gov/pubmed/33519280
http://dx.doi.org/10.2141/jpsa.0200016
work_keys_str_mv AT taharayutaka exovoculturesystemforavianembryosanditsapplication
AT obarakatsuya exovoculturesystemforavianembryosanditsapplication