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Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human

BACKGROUND: The human gut microbiota has emerged as a key factor in the development of obesity. Certain probiotic strains have shown anti-obesity effects. The objective of this study was to investigate whether Bifidobacterium longum APC1472 has anti-obesity effects in high-fat diet (HFD)-induced obe...

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Autores principales: Schellekens, Harriët, Torres-Fuentes, Cristina, van de Wouw, Marcel, Long-Smith, Caitriona M., Mitchell, Avery, Strain, Conall, Berding, Kirsten, Bastiaanssen, Thomaz F.S., Rea, Kieran, Golubeva, Anna V., Arboleya, Silvia, Verpaalen, Mathieu, Pusceddu, Matteo M., Murphy, Amy, Fouhy, Fiona, Murphy, Kiera, Ross, Paul, Roy, Bernard L., Stanton, Catherine, Dinan, Timothy G., Cryan, John F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838052/
https://www.ncbi.nlm.nih.gov/pubmed/33349590
http://dx.doi.org/10.1016/j.ebiom.2020.103176
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author Schellekens, Harriët
Torres-Fuentes, Cristina
van de Wouw, Marcel
Long-Smith, Caitriona M.
Mitchell, Avery
Strain, Conall
Berding, Kirsten
Bastiaanssen, Thomaz F.S.
Rea, Kieran
Golubeva, Anna V.
Arboleya, Silvia
Verpaalen, Mathieu
Pusceddu, Matteo M.
Murphy, Amy
Fouhy, Fiona
Murphy, Kiera
Ross, Paul
Roy, Bernard L.
Stanton, Catherine
Dinan, Timothy G.
Cryan, John F.
author_facet Schellekens, Harriët
Torres-Fuentes, Cristina
van de Wouw, Marcel
Long-Smith, Caitriona M.
Mitchell, Avery
Strain, Conall
Berding, Kirsten
Bastiaanssen, Thomaz F.S.
Rea, Kieran
Golubeva, Anna V.
Arboleya, Silvia
Verpaalen, Mathieu
Pusceddu, Matteo M.
Murphy, Amy
Fouhy, Fiona
Murphy, Kiera
Ross, Paul
Roy, Bernard L.
Stanton, Catherine
Dinan, Timothy G.
Cryan, John F.
author_sort Schellekens, Harriët
collection PubMed
description BACKGROUND: The human gut microbiota has emerged as a key factor in the development of obesity. Certain probiotic strains have shown anti-obesity effects. The objective of this study was to investigate whether Bifidobacterium longum APC1472 has anti-obesity effects in high-fat diet (HFD)-induced obese mice and whether B. longum APC1472 supplementation reduces body-mass index (BMI) in healthy overweight/obese individuals as the primary outcome. B. longum APC1472 effects on waist-to-hip ratio (W/H ratio) and on obesity-associated plasma biomarkers were analysed as secondary outcomes. METHODS: B. longum APC1472 was administered to HFD-fed C57BL/6 mice in drinking water for 16 weeks. In the human intervention trial, participants received B. longum APC1472 or placebo supplementation for 12 weeks, during which primary and secondary outcomes were measured at the beginning and end of the intervention. FINDINGS: B. longum APC1472 supplementation was associated with decreased bodyweight, fat depots accumulation and increased glucose tolerance in HFD-fed mice. While, in healthy overweight/obese adults, the supplementation of B. longum APC1472 strain did not change primary outcomes of BMI (0.03, 95% CI [-0.4, 0.3]) or W/H ratio (0.003, 95% CI [-0.01, 0.01]), a positive effect on the secondary outcome of fasting blood glucose levels was found (-0.299, 95% CI [-0.44, -0.09]). INTERPRETATION: This study shows a positive translational effect of B. longum APC1472 on fasting blood glucose from a preclinical mouse model of obesity to a human intervention study in otherwise healthy overweight and obese individuals. This highlights the promising potential of B. longum APC1472 to be developed as a valuable supplement in reducing specific markers of obesity. FUNDING: This research was funded in part by Science Foundation Ireland in the form of a Research Centre grant (SFI/12/RC/2273) to APC Microbiome Ireland and by a research grant from Cremo S.A.
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spelling pubmed-78380522021-02-01 Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human Schellekens, Harriët Torres-Fuentes, Cristina van de Wouw, Marcel Long-Smith, Caitriona M. Mitchell, Avery Strain, Conall Berding, Kirsten Bastiaanssen, Thomaz F.S. Rea, Kieran Golubeva, Anna V. Arboleya, Silvia Verpaalen, Mathieu Pusceddu, Matteo M. Murphy, Amy Fouhy, Fiona Murphy, Kiera Ross, Paul Roy, Bernard L. Stanton, Catherine Dinan, Timothy G. Cryan, John F. EBioMedicine Research Paper BACKGROUND: The human gut microbiota has emerged as a key factor in the development of obesity. Certain probiotic strains have shown anti-obesity effects. The objective of this study was to investigate whether Bifidobacterium longum APC1472 has anti-obesity effects in high-fat diet (HFD)-induced obese mice and whether B. longum APC1472 supplementation reduces body-mass index (BMI) in healthy overweight/obese individuals as the primary outcome. B. longum APC1472 effects on waist-to-hip ratio (W/H ratio) and on obesity-associated plasma biomarkers were analysed as secondary outcomes. METHODS: B. longum APC1472 was administered to HFD-fed C57BL/6 mice in drinking water for 16 weeks. In the human intervention trial, participants received B. longum APC1472 or placebo supplementation for 12 weeks, during which primary and secondary outcomes were measured at the beginning and end of the intervention. FINDINGS: B. longum APC1472 supplementation was associated with decreased bodyweight, fat depots accumulation and increased glucose tolerance in HFD-fed mice. While, in healthy overweight/obese adults, the supplementation of B. longum APC1472 strain did not change primary outcomes of BMI (0.03, 95% CI [-0.4, 0.3]) or W/H ratio (0.003, 95% CI [-0.01, 0.01]), a positive effect on the secondary outcome of fasting blood glucose levels was found (-0.299, 95% CI [-0.44, -0.09]). INTERPRETATION: This study shows a positive translational effect of B. longum APC1472 on fasting blood glucose from a preclinical mouse model of obesity to a human intervention study in otherwise healthy overweight and obese individuals. This highlights the promising potential of B. longum APC1472 to be developed as a valuable supplement in reducing specific markers of obesity. FUNDING: This research was funded in part by Science Foundation Ireland in the form of a Research Centre grant (SFI/12/RC/2273) to APC Microbiome Ireland and by a research grant from Cremo S.A. Elsevier 2020-12-18 /pmc/articles/PMC7838052/ /pubmed/33349590 http://dx.doi.org/10.1016/j.ebiom.2020.103176 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Paper
Schellekens, Harriët
Torres-Fuentes, Cristina
van de Wouw, Marcel
Long-Smith, Caitriona M.
Mitchell, Avery
Strain, Conall
Berding, Kirsten
Bastiaanssen, Thomaz F.S.
Rea, Kieran
Golubeva, Anna V.
Arboleya, Silvia
Verpaalen, Mathieu
Pusceddu, Matteo M.
Murphy, Amy
Fouhy, Fiona
Murphy, Kiera
Ross, Paul
Roy, Bernard L.
Stanton, Catherine
Dinan, Timothy G.
Cryan, John F.
Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human
title Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human
title_full Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human
title_fullStr Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human
title_full_unstemmed Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human
title_short Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human
title_sort bifidobacterium longum counters the effects of obesity: partial successful translation from rodent to human
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838052/
https://www.ncbi.nlm.nih.gov/pubmed/33349590
http://dx.doi.org/10.1016/j.ebiom.2020.103176
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