Cargando…
Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human
BACKGROUND: The human gut microbiota has emerged as a key factor in the development of obesity. Certain probiotic strains have shown anti-obesity effects. The objective of this study was to investigate whether Bifidobacterium longum APC1472 has anti-obesity effects in high-fat diet (HFD)-induced obe...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838052/ https://www.ncbi.nlm.nih.gov/pubmed/33349590 http://dx.doi.org/10.1016/j.ebiom.2020.103176 |
_version_ | 1783643085459161088 |
---|---|
author | Schellekens, Harriët Torres-Fuentes, Cristina van de Wouw, Marcel Long-Smith, Caitriona M. Mitchell, Avery Strain, Conall Berding, Kirsten Bastiaanssen, Thomaz F.S. Rea, Kieran Golubeva, Anna V. Arboleya, Silvia Verpaalen, Mathieu Pusceddu, Matteo M. Murphy, Amy Fouhy, Fiona Murphy, Kiera Ross, Paul Roy, Bernard L. Stanton, Catherine Dinan, Timothy G. Cryan, John F. |
author_facet | Schellekens, Harriët Torres-Fuentes, Cristina van de Wouw, Marcel Long-Smith, Caitriona M. Mitchell, Avery Strain, Conall Berding, Kirsten Bastiaanssen, Thomaz F.S. Rea, Kieran Golubeva, Anna V. Arboleya, Silvia Verpaalen, Mathieu Pusceddu, Matteo M. Murphy, Amy Fouhy, Fiona Murphy, Kiera Ross, Paul Roy, Bernard L. Stanton, Catherine Dinan, Timothy G. Cryan, John F. |
author_sort | Schellekens, Harriët |
collection | PubMed |
description | BACKGROUND: The human gut microbiota has emerged as a key factor in the development of obesity. Certain probiotic strains have shown anti-obesity effects. The objective of this study was to investigate whether Bifidobacterium longum APC1472 has anti-obesity effects in high-fat diet (HFD)-induced obese mice and whether B. longum APC1472 supplementation reduces body-mass index (BMI) in healthy overweight/obese individuals as the primary outcome. B. longum APC1472 effects on waist-to-hip ratio (W/H ratio) and on obesity-associated plasma biomarkers were analysed as secondary outcomes. METHODS: B. longum APC1472 was administered to HFD-fed C57BL/6 mice in drinking water for 16 weeks. In the human intervention trial, participants received B. longum APC1472 or placebo supplementation for 12 weeks, during which primary and secondary outcomes were measured at the beginning and end of the intervention. FINDINGS: B. longum APC1472 supplementation was associated with decreased bodyweight, fat depots accumulation and increased glucose tolerance in HFD-fed mice. While, in healthy overweight/obese adults, the supplementation of B. longum APC1472 strain did not change primary outcomes of BMI (0.03, 95% CI [-0.4, 0.3]) or W/H ratio (0.003, 95% CI [-0.01, 0.01]), a positive effect on the secondary outcome of fasting blood glucose levels was found (-0.299, 95% CI [-0.44, -0.09]). INTERPRETATION: This study shows a positive translational effect of B. longum APC1472 on fasting blood glucose from a preclinical mouse model of obesity to a human intervention study in otherwise healthy overweight and obese individuals. This highlights the promising potential of B. longum APC1472 to be developed as a valuable supplement in reducing specific markers of obesity. FUNDING: This research was funded in part by Science Foundation Ireland in the form of a Research Centre grant (SFI/12/RC/2273) to APC Microbiome Ireland and by a research grant from Cremo S.A. |
format | Online Article Text |
id | pubmed-7838052 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-78380522021-02-01 Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human Schellekens, Harriët Torres-Fuentes, Cristina van de Wouw, Marcel Long-Smith, Caitriona M. Mitchell, Avery Strain, Conall Berding, Kirsten Bastiaanssen, Thomaz F.S. Rea, Kieran Golubeva, Anna V. Arboleya, Silvia Verpaalen, Mathieu Pusceddu, Matteo M. Murphy, Amy Fouhy, Fiona Murphy, Kiera Ross, Paul Roy, Bernard L. Stanton, Catherine Dinan, Timothy G. Cryan, John F. EBioMedicine Research Paper BACKGROUND: The human gut microbiota has emerged as a key factor in the development of obesity. Certain probiotic strains have shown anti-obesity effects. The objective of this study was to investigate whether Bifidobacterium longum APC1472 has anti-obesity effects in high-fat diet (HFD)-induced obese mice and whether B. longum APC1472 supplementation reduces body-mass index (BMI) in healthy overweight/obese individuals as the primary outcome. B. longum APC1472 effects on waist-to-hip ratio (W/H ratio) and on obesity-associated plasma biomarkers were analysed as secondary outcomes. METHODS: B. longum APC1472 was administered to HFD-fed C57BL/6 mice in drinking water for 16 weeks. In the human intervention trial, participants received B. longum APC1472 or placebo supplementation for 12 weeks, during which primary and secondary outcomes were measured at the beginning and end of the intervention. FINDINGS: B. longum APC1472 supplementation was associated with decreased bodyweight, fat depots accumulation and increased glucose tolerance in HFD-fed mice. While, in healthy overweight/obese adults, the supplementation of B. longum APC1472 strain did not change primary outcomes of BMI (0.03, 95% CI [-0.4, 0.3]) or W/H ratio (0.003, 95% CI [-0.01, 0.01]), a positive effect on the secondary outcome of fasting blood glucose levels was found (-0.299, 95% CI [-0.44, -0.09]). INTERPRETATION: This study shows a positive translational effect of B. longum APC1472 on fasting blood glucose from a preclinical mouse model of obesity to a human intervention study in otherwise healthy overweight and obese individuals. This highlights the promising potential of B. longum APC1472 to be developed as a valuable supplement in reducing specific markers of obesity. FUNDING: This research was funded in part by Science Foundation Ireland in the form of a Research Centre grant (SFI/12/RC/2273) to APC Microbiome Ireland and by a research grant from Cremo S.A. Elsevier 2020-12-18 /pmc/articles/PMC7838052/ /pubmed/33349590 http://dx.doi.org/10.1016/j.ebiom.2020.103176 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Paper Schellekens, Harriët Torres-Fuentes, Cristina van de Wouw, Marcel Long-Smith, Caitriona M. Mitchell, Avery Strain, Conall Berding, Kirsten Bastiaanssen, Thomaz F.S. Rea, Kieran Golubeva, Anna V. Arboleya, Silvia Verpaalen, Mathieu Pusceddu, Matteo M. Murphy, Amy Fouhy, Fiona Murphy, Kiera Ross, Paul Roy, Bernard L. Stanton, Catherine Dinan, Timothy G. Cryan, John F. Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human |
title | Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human |
title_full | Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human |
title_fullStr | Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human |
title_full_unstemmed | Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human |
title_short | Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human |
title_sort | bifidobacterium longum counters the effects of obesity: partial successful translation from rodent to human |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838052/ https://www.ncbi.nlm.nih.gov/pubmed/33349590 http://dx.doi.org/10.1016/j.ebiom.2020.103176 |
work_keys_str_mv | AT schellekensharriet bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT torresfuentescristina bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT vandewouwmarcel bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT longsmithcaitrionam bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT mitchellavery bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT strainconall bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT berdingkirsten bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT bastiaanssenthomazfs bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT reakieran bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT golubevaannav bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT arboleyasilvia bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT verpaalenmathieu bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT pusceddumatteom bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT murphyamy bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT fouhyfiona bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT murphykiera bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT rosspaul bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT roybernardl bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT stantoncatherine bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT dinantimothyg bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman AT cryanjohnf bifidobacteriumlongumcounterstheeffectsofobesitypartialsuccessfultranslationfromrodenttohuman |