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Doxorubicin Inhibits Proliferation of Osteosarcoma Cells Through Upregulation of the Notch Signaling Pathway

Doxorubicin plays a major role in the treatment of osteosarcoma disorders. The Notch signaling pathway exerts various biological functions, including cell proliferation, differentiation, and apoptosis. In the present study, we investigated the effects of different doses of doxorubicin on proliferati...

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Autores principales: Ji, Peng, Yu, Ling, Guo, Wei-Chun, Mei, Hong-Jun, Wang, Xiao-Ju, Chen, Hu, Fang, Shuo, Yang, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838446/
https://www.ncbi.nlm.nih.gov/pubmed/26351207
http://dx.doi.org/10.3727/096504015X14343704124340
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author Ji, Peng
Yu, Ling
Guo, Wei-Chun
Mei, Hong-Jun
Wang, Xiao-Ju
Chen, Hu
Fang, Shuo
Yang, Jian
author_facet Ji, Peng
Yu, Ling
Guo, Wei-Chun
Mei, Hong-Jun
Wang, Xiao-Ju
Chen, Hu
Fang, Shuo
Yang, Jian
author_sort Ji, Peng
collection PubMed
description Doxorubicin plays a major role in the treatment of osteosarcoma disorders. The Notch signaling pathway exerts various biological functions, including cell proliferation, differentiation, and apoptosis. In the present study, we investigated the effects of different doses of doxorubicin on proliferation and apoptosis of osteosarcoma cells with or without Notch signaling. Results found that cellular viability was downregulated while caspase 3 activity and expression were promoted in osteosarcoma cells following treatment with various doses of doxorubicin for 24, 48, and 72 h, and the effects showed a dose- and time-dependent manner. Furthermore, it was found that various doses of doxorubicin activated the Notch signaling pathway, shown by the elevated expression of Notch target genes NOTCH1, HEY1, HES1, AND HES5. It was further proved that, after small interfering RNA (siRNA)-mediated knockdown of Notch, the effects of doxorubicin on the viability and apoptosis of osteosarcoma cells were significantly reduced. It was indicated that doxorubicin treatment reduced the proliferation and promoted the apoptosis of osteosarcoma cells, and this effect was mediated by the Notch signaling pathway.
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spelling pubmed-78384462021-02-16 Doxorubicin Inhibits Proliferation of Osteosarcoma Cells Through Upregulation of the Notch Signaling Pathway Ji, Peng Yu, Ling Guo, Wei-Chun Mei, Hong-Jun Wang, Xiao-Ju Chen, Hu Fang, Shuo Yang, Jian Oncol Res Article Doxorubicin plays a major role in the treatment of osteosarcoma disorders. The Notch signaling pathway exerts various biological functions, including cell proliferation, differentiation, and apoptosis. In the present study, we investigated the effects of different doses of doxorubicin on proliferation and apoptosis of osteosarcoma cells with or without Notch signaling. Results found that cellular viability was downregulated while caspase 3 activity and expression were promoted in osteosarcoma cells following treatment with various doses of doxorubicin for 24, 48, and 72 h, and the effects showed a dose- and time-dependent manner. Furthermore, it was found that various doses of doxorubicin activated the Notch signaling pathway, shown by the elevated expression of Notch target genes NOTCH1, HEY1, HES1, AND HES5. It was further proved that, after small interfering RNA (siRNA)-mediated knockdown of Notch, the effects of doxorubicin on the viability and apoptosis of osteosarcoma cells were significantly reduced. It was indicated that doxorubicin treatment reduced the proliferation and promoted the apoptosis of osteosarcoma cells, and this effect was mediated by the Notch signaling pathway. Cognizant Communication Corporation 2015-09-15 /pmc/articles/PMC7838446/ /pubmed/26351207 http://dx.doi.org/10.3727/096504015X14343704124340 Text en Copyright © 2015 Cognizant Comm. Corp. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Ji, Peng
Yu, Ling
Guo, Wei-Chun
Mei, Hong-Jun
Wang, Xiao-Ju
Chen, Hu
Fang, Shuo
Yang, Jian
Doxorubicin Inhibits Proliferation of Osteosarcoma Cells Through Upregulation of the Notch Signaling Pathway
title Doxorubicin Inhibits Proliferation of Osteosarcoma Cells Through Upregulation of the Notch Signaling Pathway
title_full Doxorubicin Inhibits Proliferation of Osteosarcoma Cells Through Upregulation of the Notch Signaling Pathway
title_fullStr Doxorubicin Inhibits Proliferation of Osteosarcoma Cells Through Upregulation of the Notch Signaling Pathway
title_full_unstemmed Doxorubicin Inhibits Proliferation of Osteosarcoma Cells Through Upregulation of the Notch Signaling Pathway
title_short Doxorubicin Inhibits Proliferation of Osteosarcoma Cells Through Upregulation of the Notch Signaling Pathway
title_sort doxorubicin inhibits proliferation of osteosarcoma cells through upregulation of the notch signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838446/
https://www.ncbi.nlm.nih.gov/pubmed/26351207
http://dx.doi.org/10.3727/096504015X14343704124340
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