Cargando…
Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo
Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen Staphylococcus aureus has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by S. aureu...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838569/ https://www.ncbi.nlm.nih.gov/pubmed/33521050 http://dx.doi.org/10.3389/fmolb.2020.566207 |
_version_ | 1783643209556033536 |
---|---|
author | Joshi, Bishnu Singh, Bhupender Nadeem, Aftab Askarian, Fatemeh Wai, Sun Nyunt Johannessen, Mona Hegstad, Kristin |
author_facet | Joshi, Bishnu Singh, Bhupender Nadeem, Aftab Askarian, Fatemeh Wai, Sun Nyunt Johannessen, Mona Hegstad, Kristin |
author_sort | Joshi, Bishnu |
collection | PubMed |
description | Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen Staphylococcus aureus has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by S. aureus MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our finding invites new insights into the potential role of EV-associated RNA as a modulator of host-pathogen interaction. |
format | Online Article Text |
id | pubmed-7838569 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78385692021-01-28 Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo Joshi, Bishnu Singh, Bhupender Nadeem, Aftab Askarian, Fatemeh Wai, Sun Nyunt Johannessen, Mona Hegstad, Kristin Front Mol Biosci Molecular Biosciences Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen Staphylococcus aureus has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by S. aureus MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our finding invites new insights into the potential role of EV-associated RNA as a modulator of host-pathogen interaction. Frontiers Media S.A. 2021-01-13 /pmc/articles/PMC7838569/ /pubmed/33521050 http://dx.doi.org/10.3389/fmolb.2020.566207 Text en Copyright © 2021 Joshi, Singh, Nadeem, Askarian, Wai, Johannessen and Hegstad. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Joshi, Bishnu Singh, Bhupender Nadeem, Aftab Askarian, Fatemeh Wai, Sun Nyunt Johannessen, Mona Hegstad, Kristin Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo |
title | Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo |
title_full | Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo |
title_fullStr | Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo |
title_full_unstemmed | Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo |
title_short | Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo |
title_sort | transcriptome profiling of staphylococcus aureus associated extracellular vesicles reveals presence of small rna-cargo |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838569/ https://www.ncbi.nlm.nih.gov/pubmed/33521050 http://dx.doi.org/10.3389/fmolb.2020.566207 |
work_keys_str_mv | AT joshibishnu transcriptomeprofilingofstaphylococcusaureusassociatedextracellularvesiclesrevealspresenceofsmallrnacargo AT singhbhupender transcriptomeprofilingofstaphylococcusaureusassociatedextracellularvesiclesrevealspresenceofsmallrnacargo AT nadeemaftab transcriptomeprofilingofstaphylococcusaureusassociatedextracellularvesiclesrevealspresenceofsmallrnacargo AT askarianfatemeh transcriptomeprofilingofstaphylococcusaureusassociatedextracellularvesiclesrevealspresenceofsmallrnacargo AT waisunnyunt transcriptomeprofilingofstaphylococcusaureusassociatedextracellularvesiclesrevealspresenceofsmallrnacargo AT johannessenmona transcriptomeprofilingofstaphylococcusaureusassociatedextracellularvesiclesrevealspresenceofsmallrnacargo AT hegstadkristin transcriptomeprofilingofstaphylococcusaureusassociatedextracellularvesiclesrevealspresenceofsmallrnacargo |