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Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo

Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen Staphylococcus aureus has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by S. aureu...

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Autores principales: Joshi, Bishnu, Singh, Bhupender, Nadeem, Aftab, Askarian, Fatemeh, Wai, Sun Nyunt, Johannessen, Mona, Hegstad, Kristin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838569/
https://www.ncbi.nlm.nih.gov/pubmed/33521050
http://dx.doi.org/10.3389/fmolb.2020.566207
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author Joshi, Bishnu
Singh, Bhupender
Nadeem, Aftab
Askarian, Fatemeh
Wai, Sun Nyunt
Johannessen, Mona
Hegstad, Kristin
author_facet Joshi, Bishnu
Singh, Bhupender
Nadeem, Aftab
Askarian, Fatemeh
Wai, Sun Nyunt
Johannessen, Mona
Hegstad, Kristin
author_sort Joshi, Bishnu
collection PubMed
description Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen Staphylococcus aureus has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by S. aureus MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our finding invites new insights into the potential role of EV-associated RNA as a modulator of host-pathogen interaction.
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spelling pubmed-78385692021-01-28 Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo Joshi, Bishnu Singh, Bhupender Nadeem, Aftab Askarian, Fatemeh Wai, Sun Nyunt Johannessen, Mona Hegstad, Kristin Front Mol Biosci Molecular Biosciences Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen Staphylococcus aureus has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by S. aureus MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our finding invites new insights into the potential role of EV-associated RNA as a modulator of host-pathogen interaction. Frontiers Media S.A. 2021-01-13 /pmc/articles/PMC7838569/ /pubmed/33521050 http://dx.doi.org/10.3389/fmolb.2020.566207 Text en Copyright © 2021 Joshi, Singh, Nadeem, Askarian, Wai, Johannessen and Hegstad. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Joshi, Bishnu
Singh, Bhupender
Nadeem, Aftab
Askarian, Fatemeh
Wai, Sun Nyunt
Johannessen, Mona
Hegstad, Kristin
Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo
title Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo
title_full Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo
title_fullStr Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo
title_full_unstemmed Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo
title_short Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo
title_sort transcriptome profiling of staphylococcus aureus associated extracellular vesicles reveals presence of small rna-cargo
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838569/
https://www.ncbi.nlm.nih.gov/pubmed/33521050
http://dx.doi.org/10.3389/fmolb.2020.566207
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