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Severe acute respiratory syndrome coronavirus 2 detected in placentas of 2 coronavirus disease 2019–positive asymptomatic pregnant women—case report

There is limited evidence regarding severe acute respiratory syndrome coronavirus 2 infection in the placenta of pregnant women who tested positive, and if this could be a route for vertical transmission of the virus in utero. We present the cases of 2 pregnant women in their third trimester who wer...

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Autores principales: Sanchez, Jaime, Vigil-De Gracia, Paulino, Guerrero, Erika, Gaitán, Melissa, Fu, Cindy, Chen-Germán, María, Villalobos, Rodrigo, Coronado, Luis, Martínez, Alexander A., Araúz, Dimelza, Saenz, Lisseth, Chavarría, Oris, Góndola, Jessica, Moreno, Ambar, González, Claudia, Vega, Shantal, Campana, Sara, Ng Chinkee, Jorge, López-Vergès, Sandra, Solís, Mairim Alexandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838577/
https://www.ncbi.nlm.nih.gov/pubmed/33842905
http://dx.doi.org/10.1016/j.xagr.2020.100001
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author Sanchez, Jaime
Vigil-De Gracia, Paulino
Guerrero, Erika
Gaitán, Melissa
Fu, Cindy
Chen-Germán, María
Villalobos, Rodrigo
Coronado, Luis
Martínez, Alexander A.
Araúz, Dimelza
Saenz, Lisseth
Chavarría, Oris
Góndola, Jessica
Moreno, Ambar
González, Claudia
Vega, Shantal
Campana, Sara
Ng Chinkee, Jorge
López-Vergès, Sandra
Solís, Mairim Alexandra
author_facet Sanchez, Jaime
Vigil-De Gracia, Paulino
Guerrero, Erika
Gaitán, Melissa
Fu, Cindy
Chen-Germán, María
Villalobos, Rodrigo
Coronado, Luis
Martínez, Alexander A.
Araúz, Dimelza
Saenz, Lisseth
Chavarría, Oris
Góndola, Jessica
Moreno, Ambar
González, Claudia
Vega, Shantal
Campana, Sara
Ng Chinkee, Jorge
López-Vergès, Sandra
Solís, Mairim Alexandra
author_sort Sanchez, Jaime
collection PubMed
description There is limited evidence regarding severe acute respiratory syndrome coronavirus 2 infection in the placenta of pregnant women who tested positive, and if this could be a route for vertical transmission of the virus in utero. We present the cases of 2 pregnant women in their third trimester who were admitted for delivery by cesarean delivery and who, through universal screening, tested positive for coronavirus disease 2019. The maternal and fetal sides of the placenta were sectioned from both patients for viral analysis. Real-time polymerase chain reaction analysis of the placental-extracted RNA revealed a severe acute respiratory syndrome coronavirus 2 infection on the fetal side of the placenta in both patients. The virus was isolated from the patient with the lowest cycle threshold value on the fetal side of the placenta. Whole genome sequencing showed that the virus detected in this placenta was from the B1 lineage. Immunohistochemical analysis of the placental tissue detected severe acute respiratory syndrome coronavirus 2 in the endothelial cells of chorionic villi vessels proximal to both the maternal and fetal sides, with a granular cytoplasmic pattern and perinuclear reinforcement. Histologic examination of the placenta also detected a dense infiltrate of lymphoid cells around decidual vessels and endothelial cells with cytopathic changes, especially on the maternal side. Nasopharyngeal swabs from the infants that were subjected to reverse transcription quantitative polymerase chain reaction testing were negative for severe acute respiratory syndrome coronavirus 2 at 24 hours after birth. A follow-up analysis of the infants for immunoglobin G and immunoglobin M expression, clinical manifestations, and long-term developmental abnormalities is recommended.
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spelling pubmed-78385772021-01-27 Severe acute respiratory syndrome coronavirus 2 detected in placentas of 2 coronavirus disease 2019–positive asymptomatic pregnant women—case report Sanchez, Jaime Vigil-De Gracia, Paulino Guerrero, Erika Gaitán, Melissa Fu, Cindy Chen-Germán, María Villalobos, Rodrigo Coronado, Luis Martínez, Alexander A. Araúz, Dimelza Saenz, Lisseth Chavarría, Oris Góndola, Jessica Moreno, Ambar González, Claudia Vega, Shantal Campana, Sara Ng Chinkee, Jorge López-Vergès, Sandra Solís, Mairim Alexandra AJOG Glob Rep Case Report There is limited evidence regarding severe acute respiratory syndrome coronavirus 2 infection in the placenta of pregnant women who tested positive, and if this could be a route for vertical transmission of the virus in utero. We present the cases of 2 pregnant women in their third trimester who were admitted for delivery by cesarean delivery and who, through universal screening, tested positive for coronavirus disease 2019. The maternal and fetal sides of the placenta were sectioned from both patients for viral analysis. Real-time polymerase chain reaction analysis of the placental-extracted RNA revealed a severe acute respiratory syndrome coronavirus 2 infection on the fetal side of the placenta in both patients. The virus was isolated from the patient with the lowest cycle threshold value on the fetal side of the placenta. Whole genome sequencing showed that the virus detected in this placenta was from the B1 lineage. Immunohistochemical analysis of the placental tissue detected severe acute respiratory syndrome coronavirus 2 in the endothelial cells of chorionic villi vessels proximal to both the maternal and fetal sides, with a granular cytoplasmic pattern and perinuclear reinforcement. Histologic examination of the placenta also detected a dense infiltrate of lymphoid cells around decidual vessels and endothelial cells with cytopathic changes, especially on the maternal side. Nasopharyngeal swabs from the infants that were subjected to reverse transcription quantitative polymerase chain reaction testing were negative for severe acute respiratory syndrome coronavirus 2 at 24 hours after birth. A follow-up analysis of the infants for immunoglobin G and immunoglobin M expression, clinical manifestations, and long-term developmental abnormalities is recommended. Elsevier 2020-12-16 /pmc/articles/PMC7838577/ /pubmed/33842905 http://dx.doi.org/10.1016/j.xagr.2020.100001 Text en © 2020 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Case Report
Sanchez, Jaime
Vigil-De Gracia, Paulino
Guerrero, Erika
Gaitán, Melissa
Fu, Cindy
Chen-Germán, María
Villalobos, Rodrigo
Coronado, Luis
Martínez, Alexander A.
Araúz, Dimelza
Saenz, Lisseth
Chavarría, Oris
Góndola, Jessica
Moreno, Ambar
González, Claudia
Vega, Shantal
Campana, Sara
Ng Chinkee, Jorge
López-Vergès, Sandra
Solís, Mairim Alexandra
Severe acute respiratory syndrome coronavirus 2 detected in placentas of 2 coronavirus disease 2019–positive asymptomatic pregnant women—case report
title Severe acute respiratory syndrome coronavirus 2 detected in placentas of 2 coronavirus disease 2019–positive asymptomatic pregnant women—case report
title_full Severe acute respiratory syndrome coronavirus 2 detected in placentas of 2 coronavirus disease 2019–positive asymptomatic pregnant women—case report
title_fullStr Severe acute respiratory syndrome coronavirus 2 detected in placentas of 2 coronavirus disease 2019–positive asymptomatic pregnant women—case report
title_full_unstemmed Severe acute respiratory syndrome coronavirus 2 detected in placentas of 2 coronavirus disease 2019–positive asymptomatic pregnant women—case report
title_short Severe acute respiratory syndrome coronavirus 2 detected in placentas of 2 coronavirus disease 2019–positive asymptomatic pregnant women—case report
title_sort severe acute respiratory syndrome coronavirus 2 detected in placentas of 2 coronavirus disease 2019–positive asymptomatic pregnant women—case report
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838577/
https://www.ncbi.nlm.nih.gov/pubmed/33842905
http://dx.doi.org/10.1016/j.xagr.2020.100001
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