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A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish

Previous studies have suggested that 1,9-Pyrazoloanthrone, known as SP600125, can induce cell polyploidization. However, what is the phase of cell cycle arrest caused by SP600125 and the underlying regulation is still an interesting issue to be further addressed. Research in this article shows that...

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Autores principales: Xu, Wenting, Mo, Yanxiu, He, Yu, Fan, Yunpeng, He, Guomin, Fu, Wen, Chen, Shujuan, Liu, Jinhui, Liu, Wenbin, Peng, Liangyue, Xiao, Yamei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838586/
https://www.ncbi.nlm.nih.gov/pubmed/33520960
http://dx.doi.org/10.3389/fbioe.2020.606496
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author Xu, Wenting
Mo, Yanxiu
He, Yu
Fan, Yunpeng
He, Guomin
Fu, Wen
Chen, Shujuan
Liu, Jinhui
Liu, Wenbin
Peng, Liangyue
Xiao, Yamei
author_facet Xu, Wenting
Mo, Yanxiu
He, Yu
Fan, Yunpeng
He, Guomin
Fu, Wen
Chen, Shujuan
Liu, Jinhui
Liu, Wenbin
Peng, Liangyue
Xiao, Yamei
author_sort Xu, Wenting
collection PubMed
description Previous studies have suggested that 1,9-Pyrazoloanthrone, known as SP600125, can induce cell polyploidization. However, what is the phase of cell cycle arrest caused by SP600125 and the underlying regulation is still an interesting issue to be further addressed. Research in this article shows that SP600125 can block cell cycle progression at the prometaphase of mitosis and cause endomitosis. It is suggested that enhancement of the p53 signaling pathway and weakening of the spindle assembly checkpoint are associated with the SP600125-induced cell cycle arrest. Using preliminary SP600125 treatment, the samples of the cultured fish cells and the fish tissues display a great number of chromosome splitting phases. Summarily, SP600125 can provide a new protocol of chromosomes preparation for karyotype analysis owing to its interference with prometaphase of mitosis.
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spelling pubmed-78385862021-01-28 A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish Xu, Wenting Mo, Yanxiu He, Yu Fan, Yunpeng He, Guomin Fu, Wen Chen, Shujuan Liu, Jinhui Liu, Wenbin Peng, Liangyue Xiao, Yamei Front Bioeng Biotechnol Bioengineering and Biotechnology Previous studies have suggested that 1,9-Pyrazoloanthrone, known as SP600125, can induce cell polyploidization. However, what is the phase of cell cycle arrest caused by SP600125 and the underlying regulation is still an interesting issue to be further addressed. Research in this article shows that SP600125 can block cell cycle progression at the prometaphase of mitosis and cause endomitosis. It is suggested that enhancement of the p53 signaling pathway and weakening of the spindle assembly checkpoint are associated with the SP600125-induced cell cycle arrest. Using preliminary SP600125 treatment, the samples of the cultured fish cells and the fish tissues display a great number of chromosome splitting phases. Summarily, SP600125 can provide a new protocol of chromosomes preparation for karyotype analysis owing to its interference with prometaphase of mitosis. Frontiers Media S.A. 2021-01-13 /pmc/articles/PMC7838586/ /pubmed/33520960 http://dx.doi.org/10.3389/fbioe.2020.606496 Text en Copyright © 2021 Xu, Mo, He, Fan, He, Fu, Chen, Liu, Liu, Peng and Xiao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Xu, Wenting
Mo, Yanxiu
He, Yu
Fan, Yunpeng
He, Guomin
Fu, Wen
Chen, Shujuan
Liu, Jinhui
Liu, Wenbin
Peng, Liangyue
Xiao, Yamei
A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish
title A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish
title_full A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish
title_fullStr A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish
title_full_unstemmed A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish
title_short A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish
title_sort new method for chromosomes preparation by atp-competitive inhibitor sp600125 via enhancement of endomitosis in fish
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838586/
https://www.ncbi.nlm.nih.gov/pubmed/33520960
http://dx.doi.org/10.3389/fbioe.2020.606496
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