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miR-489 Suppresses Proliferation and Invasion of Human Bladder Cancer Cells

MicroRNAs (miRNAs) have been shown to be involved in bladder cancer progression. miR-489 (also known as miR-489-3p) was recently reported to be a tumor suppressor in several cancers. However, its exact role and mechanism in the progression of bladder cancer are largely unknown. In this study, we exp...

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Autores principales: Li, Jing, Qu, Weixing, Jiang, Yazhou, Sun, Yi, Cheng, Yongyi, Zou, Tiejun, Du, Shuangkuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838638/
https://www.ncbi.nlm.nih.gov/pubmed/28281959
http://dx.doi.org/10.3727/096504016X14666990347518
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author Li, Jing
Qu, Weixing
Jiang, Yazhou
Sun, Yi
Cheng, Yongyi
Zou, Tiejun
Du, Shuangkuan
author_facet Li, Jing
Qu, Weixing
Jiang, Yazhou
Sun, Yi
Cheng, Yongyi
Zou, Tiejun
Du, Shuangkuan
author_sort Li, Jing
collection PubMed
description MicroRNAs (miRNAs) have been shown to be involved in bladder cancer progression. miR-489 (also known as miR-489-3p) was recently reported to be a tumor suppressor in several cancers. However, its exact role and mechanism in the progression of bladder cancer are largely unknown. In this study, we explore the role of miR-489 in the proliferation and invasion of human bladder cancer cells. The miR-489 expression levels were detected in bladder cancer and normal adjacent tissues, as well as in human normal bladder epithelial cells and bladder cancer cell lines. The results showed that miR-489 was sharply reduced in bladder cancer tissues and cell lines. Then the miR-489 mimic or oligo anta-miR-489 was transfected into T24 and UMUC3 bladder cancer cell lines. The results showed that the miR-489 mimic greatly increased the miR-489 level and significantly decreased the proliferation and invasion of T24 and UMUC3 cells. In contrast, the anta-miR-489 had a completely opposite effect on miR-489 expression, cell proliferation, and cell invasion. Moreover, bioinformatics and luciferase reporter gene assays confirmed that miR-489 targeted the mRNA 3′-untranslated region (3′-UTR) region of Jagged1 (JAG1), a Notch ligand. In conclusion, miR-489 suppressed proliferation and invasion of human bladder cancer cells.
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spelling pubmed-78386382021-02-16 miR-489 Suppresses Proliferation and Invasion of Human Bladder Cancer Cells Li, Jing Qu, Weixing Jiang, Yazhou Sun, Yi Cheng, Yongyi Zou, Tiejun Du, Shuangkuan Oncol Res Article MicroRNAs (miRNAs) have been shown to be involved in bladder cancer progression. miR-489 (also known as miR-489-3p) was recently reported to be a tumor suppressor in several cancers. However, its exact role and mechanism in the progression of bladder cancer are largely unknown. In this study, we explore the role of miR-489 in the proliferation and invasion of human bladder cancer cells. The miR-489 expression levels were detected in bladder cancer and normal adjacent tissues, as well as in human normal bladder epithelial cells and bladder cancer cell lines. The results showed that miR-489 was sharply reduced in bladder cancer tissues and cell lines. Then the miR-489 mimic or oligo anta-miR-489 was transfected into T24 and UMUC3 bladder cancer cell lines. The results showed that the miR-489 mimic greatly increased the miR-489 level and significantly decreased the proliferation and invasion of T24 and UMUC3 cells. In contrast, the anta-miR-489 had a completely opposite effect on miR-489 expression, cell proliferation, and cell invasion. Moreover, bioinformatics and luciferase reporter gene assays confirmed that miR-489 targeted the mRNA 3′-untranslated region (3′-UTR) region of Jagged1 (JAG1), a Notch ligand. In conclusion, miR-489 suppressed proliferation and invasion of human bladder cancer cells. Cognizant Communication Corporation 2016-10-27 /pmc/articles/PMC7838638/ /pubmed/28281959 http://dx.doi.org/10.3727/096504016X14666990347518 Text en Copyright © 2016 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Li, Jing
Qu, Weixing
Jiang, Yazhou
Sun, Yi
Cheng, Yongyi
Zou, Tiejun
Du, Shuangkuan
miR-489 Suppresses Proliferation and Invasion of Human Bladder Cancer Cells
title miR-489 Suppresses Proliferation and Invasion of Human Bladder Cancer Cells
title_full miR-489 Suppresses Proliferation and Invasion of Human Bladder Cancer Cells
title_fullStr miR-489 Suppresses Proliferation and Invasion of Human Bladder Cancer Cells
title_full_unstemmed miR-489 Suppresses Proliferation and Invasion of Human Bladder Cancer Cells
title_short miR-489 Suppresses Proliferation and Invasion of Human Bladder Cancer Cells
title_sort mir-489 suppresses proliferation and invasion of human bladder cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838638/
https://www.ncbi.nlm.nih.gov/pubmed/28281959
http://dx.doi.org/10.3727/096504016X14666990347518
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