Plasmid-Based Stat3 siRNA Delivered by Functional Graphene Oxide Suppresses Mouse Malignant Melanoma Cell Growth

RNA interference (RNAi) has been used for cancer gene therapy in recent years. However, the application of RNAi is hindered in the absence of safe and efficient gene delivery. In this article, a novel vehicle of graphene oxide functionalized with polyethylenimine and polyethylene glycol (GO-PEI-PEG)...

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Autores principales: Yin, Di, Li, Yang, Guo, Baofeng, Liu, Zhewen, Xu, Yang, Wang, Xiaoqin, Du, Yanwei, Xu, Libo, Meng, Yan, Zhao, Xuejian, Zhang, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838696/
https://www.ncbi.nlm.nih.gov/pubmed/27098146
http://dx.doi.org/10.3727/096504016X14550280421449
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author Yin, Di
Li, Yang
Guo, Baofeng
Liu, Zhewen
Xu, Yang
Wang, Xiaoqin
Du, Yanwei
Xu, Libo
Meng, Yan
Zhao, Xuejian
Zhang, Ling
author_facet Yin, Di
Li, Yang
Guo, Baofeng
Liu, Zhewen
Xu, Yang
Wang, Xiaoqin
Du, Yanwei
Xu, Libo
Meng, Yan
Zhao, Xuejian
Zhang, Ling
author_sort Yin, Di
collection PubMed
description RNA interference (RNAi) has been used for cancer gene therapy in recent years. However, the application of RNAi is hindered in the absence of safe and efficient gene delivery. In this article, a novel vehicle of graphene oxide functionalized with polyethylenimine and polyethylene glycol (GO-PEI-PEG) was successfully synthetized and then used to deliver plasmid-based Stat3 siRNA. The carrier can readily bind plasmid with high transfection efficiency. Moreover, molecular biology studies reveal that Stat3-related gene and protein expressions were significantly inhibited, suggesting that the formation of GO-PEI-PEG complexes could be utilized as a promising gene delivery in cancer therapy.
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spelling pubmed-78386962021-02-16 Plasmid-Based Stat3 siRNA Delivered by Functional Graphene Oxide Suppresses Mouse Malignant Melanoma Cell Growth Yin, Di Li, Yang Guo, Baofeng Liu, Zhewen Xu, Yang Wang, Xiaoqin Du, Yanwei Xu, Libo Meng, Yan Zhao, Xuejian Zhang, Ling Oncol Res Article RNA interference (RNAi) has been used for cancer gene therapy in recent years. However, the application of RNAi is hindered in the absence of safe and efficient gene delivery. In this article, a novel vehicle of graphene oxide functionalized with polyethylenimine and polyethylene glycol (GO-PEI-PEG) was successfully synthetized and then used to deliver plasmid-based Stat3 siRNA. The carrier can readily bind plasmid with high transfection efficiency. Moreover, molecular biology studies reveal that Stat3-related gene and protein expressions were significantly inhibited, suggesting that the formation of GO-PEI-PEG complexes could be utilized as a promising gene delivery in cancer therapy. Cognizant Communication Corporation 2016-04-13 /pmc/articles/PMC7838696/ /pubmed/27098146 http://dx.doi.org/10.3727/096504016X14550280421449 Text en Copyright © 2016 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Yin, Di
Li, Yang
Guo, Baofeng
Liu, Zhewen
Xu, Yang
Wang, Xiaoqin
Du, Yanwei
Xu, Libo
Meng, Yan
Zhao, Xuejian
Zhang, Ling
Plasmid-Based Stat3 siRNA Delivered by Functional Graphene Oxide Suppresses Mouse Malignant Melanoma Cell Growth
title Plasmid-Based Stat3 siRNA Delivered by Functional Graphene Oxide Suppresses Mouse Malignant Melanoma Cell Growth
title_full Plasmid-Based Stat3 siRNA Delivered by Functional Graphene Oxide Suppresses Mouse Malignant Melanoma Cell Growth
title_fullStr Plasmid-Based Stat3 siRNA Delivered by Functional Graphene Oxide Suppresses Mouse Malignant Melanoma Cell Growth
title_full_unstemmed Plasmid-Based Stat3 siRNA Delivered by Functional Graphene Oxide Suppresses Mouse Malignant Melanoma Cell Growth
title_short Plasmid-Based Stat3 siRNA Delivered by Functional Graphene Oxide Suppresses Mouse Malignant Melanoma Cell Growth
title_sort plasmid-based stat3 sirna delivered by functional graphene oxide suppresses mouse malignant melanoma cell growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838696/
https://www.ncbi.nlm.nih.gov/pubmed/27098146
http://dx.doi.org/10.3727/096504016X14550280421449
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