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Enhancement of Drug Sensitivity by Knockdown of HIF-1α in Gastric Carcinoma Cells
In this study, the effects of hypoxia-inducible factor-1α (HIF-1α) on gastric carcinoma (GC) drug resistance through apoptosis-related genes are investigated. First, HIF-1α-specific siRNA was synthetized and transfected into drug-resistant GC cell line OCUM-2MD3/L-OHP. Then MTT assay was applied to...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cognizant Communication Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838699/ https://www.ncbi.nlm.nih.gov/pubmed/26931435 http://dx.doi.org/10.3727/096504015X14500513118029 |
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author | Zhao, Qun Tan, Bi-Bo Li, Yong Fan, Li-Qiao Yang, Pei-Gang Tian, Yuan |
author_facet | Zhao, Qun Tan, Bi-Bo Li, Yong Fan, Li-Qiao Yang, Pei-Gang Tian, Yuan |
author_sort | Zhao, Qun |
collection | PubMed |
description | In this study, the effects of hypoxia-inducible factor-1α (HIF-1α) on gastric carcinoma (GC) drug resistance through apoptosis-related genes are investigated. First, HIF-1α-specific siRNA was synthetized and transfected into drug-resistant GC cell line OCUM-2MD3/L-OHP. Then MTT assay was applied to test the inhibition rate of GC cells by 5-fluorouracil (5-FU) and oxaliplatin (L-OHP). After that, flow cytometry (FCM) was applied to measure apoptosis rate. qPCR and Western blot assay were employed to detect HIF-1α and apoptosis-related genes. Results showed that HIF-1α in OCUM-2MD3/L-OHP cells was higher than that in OCUM-2MD3 and gastric epithelial cells. After HIF-1α-siRNA transfection, inhibition rates of 5-FU and L-OHP to tumor cells increased significantly. FCM results showed that apoptosis rate of OCUM-2MD3/L-OHP cells increased significantly. After HIF-1α-siRNA transfection, survivin and Bcl-2 decreased, whereas Bax, caspase 3, and caspase 8 increased significantly. Results from this study seem to confirm that HIF-1α getting involved in GC drug resistance is possibly due to its regulation of some apoptosis-related genes. HIF-1α may be a potential target to reverse drug resistance of GC. |
format | Online Article Text |
id | pubmed-7838699 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Cognizant Communication Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-78386992021-02-16 Enhancement of Drug Sensitivity by Knockdown of HIF-1α in Gastric Carcinoma Cells Zhao, Qun Tan, Bi-Bo Li, Yong Fan, Li-Qiao Yang, Pei-Gang Tian, Yuan Oncol Res Article In this study, the effects of hypoxia-inducible factor-1α (HIF-1α) on gastric carcinoma (GC) drug resistance through apoptosis-related genes are investigated. First, HIF-1α-specific siRNA was synthetized and transfected into drug-resistant GC cell line OCUM-2MD3/L-OHP. Then MTT assay was applied to test the inhibition rate of GC cells by 5-fluorouracil (5-FU) and oxaliplatin (L-OHP). After that, flow cytometry (FCM) was applied to measure apoptosis rate. qPCR and Western blot assay were employed to detect HIF-1α and apoptosis-related genes. Results showed that HIF-1α in OCUM-2MD3/L-OHP cells was higher than that in OCUM-2MD3 and gastric epithelial cells. After HIF-1α-siRNA transfection, inhibition rates of 5-FU and L-OHP to tumor cells increased significantly. FCM results showed that apoptosis rate of OCUM-2MD3/L-OHP cells increased significantly. After HIF-1α-siRNA transfection, survivin and Bcl-2 decreased, whereas Bax, caspase 3, and caspase 8 increased significantly. Results from this study seem to confirm that HIF-1α getting involved in GC drug resistance is possibly due to its regulation of some apoptosis-related genes. HIF-1α may be a potential target to reverse drug resistance of GC. Cognizant Communication Corporation 2016-02-29 /pmc/articles/PMC7838699/ /pubmed/26931435 http://dx.doi.org/10.3727/096504015X14500513118029 Text en Copyright © 2016 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License. |
spellingShingle | Article Zhao, Qun Tan, Bi-Bo Li, Yong Fan, Li-Qiao Yang, Pei-Gang Tian, Yuan Enhancement of Drug Sensitivity by Knockdown of HIF-1α in Gastric Carcinoma Cells |
title | Enhancement of Drug Sensitivity by Knockdown of HIF-1α in Gastric Carcinoma Cells |
title_full | Enhancement of Drug Sensitivity by Knockdown of HIF-1α in Gastric Carcinoma Cells |
title_fullStr | Enhancement of Drug Sensitivity by Knockdown of HIF-1α in Gastric Carcinoma Cells |
title_full_unstemmed | Enhancement of Drug Sensitivity by Knockdown of HIF-1α in Gastric Carcinoma Cells |
title_short | Enhancement of Drug Sensitivity by Knockdown of HIF-1α in Gastric Carcinoma Cells |
title_sort | enhancement of drug sensitivity by knockdown of hif-1α in gastric carcinoma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838699/ https://www.ncbi.nlm.nih.gov/pubmed/26931435 http://dx.doi.org/10.3727/096504015X14500513118029 |
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