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HSP27 Knockdown Increases Cytoplasmic p21 and Cisplatin Sensitivity in Ovarian Carcinoma Cells

Drug resistance is the leading cause of chemotherapy failure in the treatment of ovarian cancer. So far, little is known about the mechanism of chemoresistance in ovarian cancer. In this study, we explored the mechanism that HSP27 was involved in cisplatin resistance of ovarian cancer both in vitro...

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Autores principales: Lu, Hao, Sun, Chaoyang, Zhou, Ting, Zhou, Bo, Guo, Ensong, Shan, Wanying, Xia, Meng, Li, Kezhen, Weng, Danhui, Meng, Li, Xu, Xiaoyan, Hu, Junbo, Ma, Ding, Chen, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838724/
https://www.ncbi.nlm.nih.gov/pubmed/26931434
http://dx.doi.org/10.3727/096504015X14496932933656
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author Lu, Hao
Sun, Chaoyang
Zhou, Ting
Zhou, Bo
Guo, Ensong
Shan, Wanying
Xia, Meng
Li, Kezhen
Weng, Danhui
Meng, Li
Xu, Xiaoyan
Hu, Junbo
Ma, Ding
Chen, Gang
author_facet Lu, Hao
Sun, Chaoyang
Zhou, Ting
Zhou, Bo
Guo, Ensong
Shan, Wanying
Xia, Meng
Li, Kezhen
Weng, Danhui
Meng, Li
Xu, Xiaoyan
Hu, Junbo
Ma, Ding
Chen, Gang
author_sort Lu, Hao
collection PubMed
description Drug resistance is the leading cause of chemotherapy failure in the treatment of ovarian cancer. So far, little is known about the mechanism of chemoresistance in ovarian cancer. In this study, we explored the mechanism that HSP27 was involved in cisplatin resistance of ovarian cancer both in vitro and clinically. HSP27 protein was found to be upregulated and expressed in cisplatin-resistant ovarian cancer cell line C13*, and HSP27 siRNA transfection reversed the chemoresistance of C13*. We found that HSP27 exerted its chemoresistant role by inhibiting p21 transferring from the nucleus to the plasma through the activation of phosphorylated-Akt pathway. These findings have implications for clinical trials aimed at a potential therapeutic target for ovarian tumors that are refractory to conventional treatment.
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spelling pubmed-78387242021-02-16 HSP27 Knockdown Increases Cytoplasmic p21 and Cisplatin Sensitivity in Ovarian Carcinoma Cells Lu, Hao Sun, Chaoyang Zhou, Ting Zhou, Bo Guo, Ensong Shan, Wanying Xia, Meng Li, Kezhen Weng, Danhui Meng, Li Xu, Xiaoyan Hu, Junbo Ma, Ding Chen, Gang Oncol Res Article Drug resistance is the leading cause of chemotherapy failure in the treatment of ovarian cancer. So far, little is known about the mechanism of chemoresistance in ovarian cancer. In this study, we explored the mechanism that HSP27 was involved in cisplatin resistance of ovarian cancer both in vitro and clinically. HSP27 protein was found to be upregulated and expressed in cisplatin-resistant ovarian cancer cell line C13*, and HSP27 siRNA transfection reversed the chemoresistance of C13*. We found that HSP27 exerted its chemoresistant role by inhibiting p21 transferring from the nucleus to the plasma through the activation of phosphorylated-Akt pathway. These findings have implications for clinical trials aimed at a potential therapeutic target for ovarian tumors that are refractory to conventional treatment. Cognizant Communication Corporation 2016-02-29 /pmc/articles/PMC7838724/ /pubmed/26931434 http://dx.doi.org/10.3727/096504015X14496932933656 Text en Copyright © 2016 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Lu, Hao
Sun, Chaoyang
Zhou, Ting
Zhou, Bo
Guo, Ensong
Shan, Wanying
Xia, Meng
Li, Kezhen
Weng, Danhui
Meng, Li
Xu, Xiaoyan
Hu, Junbo
Ma, Ding
Chen, Gang
HSP27 Knockdown Increases Cytoplasmic p21 and Cisplatin Sensitivity in Ovarian Carcinoma Cells
title HSP27 Knockdown Increases Cytoplasmic p21 and Cisplatin Sensitivity in Ovarian Carcinoma Cells
title_full HSP27 Knockdown Increases Cytoplasmic p21 and Cisplatin Sensitivity in Ovarian Carcinoma Cells
title_fullStr HSP27 Knockdown Increases Cytoplasmic p21 and Cisplatin Sensitivity in Ovarian Carcinoma Cells
title_full_unstemmed HSP27 Knockdown Increases Cytoplasmic p21 and Cisplatin Sensitivity in Ovarian Carcinoma Cells
title_short HSP27 Knockdown Increases Cytoplasmic p21 and Cisplatin Sensitivity in Ovarian Carcinoma Cells
title_sort hsp27 knockdown increases cytoplasmic p21 and cisplatin sensitivity in ovarian carcinoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838724/
https://www.ncbi.nlm.nih.gov/pubmed/26931434
http://dx.doi.org/10.3727/096504015X14496932933656
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