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Therapeutic potential of bioactive compounds from Punica granatum extracts against aging and complicity of FOXO orthologue DAF-16 in Caenorhabditis elegans

Some natural fruits have significant importance in improving health which provides many nutritional supplements essential to maintain proper metabolism with the age. In this study, phytochemical screening of extract (methanolic) of Punica granatum arils, outer and inner peels was confirmed by the re...

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Autores principales: Chaubey, Mukesh G., Chauhan, Anita P., Chokshi, Pooja R., Amin, Rahi S., Patel, Stuti N., Madamwar, Datta, Rastogi, Rajesh P., Singh, Niraj Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Leibniz Research Centre for Working Environment and Human Factors 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838930/
https://www.ncbi.nlm.nih.gov/pubmed/33510593
http://dx.doi.org/10.17179/excli2020-3011
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author Chaubey, Mukesh G.
Chauhan, Anita P.
Chokshi, Pooja R.
Amin, Rahi S.
Patel, Stuti N.
Madamwar, Datta
Rastogi, Rajesh P.
Singh, Niraj Kumar
author_facet Chaubey, Mukesh G.
Chauhan, Anita P.
Chokshi, Pooja R.
Amin, Rahi S.
Patel, Stuti N.
Madamwar, Datta
Rastogi, Rajesh P.
Singh, Niraj Kumar
author_sort Chaubey, Mukesh G.
collection PubMed
description Some natural fruits have significant importance in improving health which provides many nutritional supplements essential to maintain proper metabolism with the age. In this study, phytochemical screening of extract (methanolic) of Punica granatum arils, outer and inner peels was confirmed by the respective spot tests. Quantification of phytochemical constituents revealed the plentiful of total phenols in the outer peels in comparison to inner peels and juice whereas total flavonoids and vitamin C are abundant in inner peel and juice, respectively. High-performance liquid chromatography, Gas chromatography along with mass spectrometry and Fourier-transform infrared spectroscopy analysis revealed the presence of compound 9, 17-octadecadienal, (Z) in the outer/inner peels. A compound N-hexadecanoic acid was also observed in the outer peels. Extracts from every section of the fruits were comprehensively evaluated for their antioxidant activity. Contrary to fruit aril juice, the extracts of outer and inner peels exhibited significant and dose-dependent in vitro antioxidant and radical-scavenging potentials. The supplementation of P. granatum extracts (PGEs) significantly enhanced the lifespan of C. elegans. The protective effect of PGEs was also observed against oxidative stress in C. elegans. Additionally, the involvement of FOXO orthologue DAF-16 dependent longevity was obtained with PGEs (outer peel and inner peel) fed TJ356 worms. Overall, the results indicate the vital role of PGEs especially the extracts of outer peels in life-saving mechanisms of C. elegans by virtue of their antioxidant asset and life-prolonging effects via daf-16 dependent Insulin signaling pathway. See also Figure 1(Fig. 1).
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spelling pubmed-78389302021-01-27 Therapeutic potential of bioactive compounds from Punica granatum extracts against aging and complicity of FOXO orthologue DAF-16 in Caenorhabditis elegans Chaubey, Mukesh G. Chauhan, Anita P. Chokshi, Pooja R. Amin, Rahi S. Patel, Stuti N. Madamwar, Datta Rastogi, Rajesh P. Singh, Niraj Kumar EXCLI J Original Article Some natural fruits have significant importance in improving health which provides many nutritional supplements essential to maintain proper metabolism with the age. In this study, phytochemical screening of extract (methanolic) of Punica granatum arils, outer and inner peels was confirmed by the respective spot tests. Quantification of phytochemical constituents revealed the plentiful of total phenols in the outer peels in comparison to inner peels and juice whereas total flavonoids and vitamin C are abundant in inner peel and juice, respectively. High-performance liquid chromatography, Gas chromatography along with mass spectrometry and Fourier-transform infrared spectroscopy analysis revealed the presence of compound 9, 17-octadecadienal, (Z) in the outer/inner peels. A compound N-hexadecanoic acid was also observed in the outer peels. Extracts from every section of the fruits were comprehensively evaluated for their antioxidant activity. Contrary to fruit aril juice, the extracts of outer and inner peels exhibited significant and dose-dependent in vitro antioxidant and radical-scavenging potentials. The supplementation of P. granatum extracts (PGEs) significantly enhanced the lifespan of C. elegans. The protective effect of PGEs was also observed against oxidative stress in C. elegans. Additionally, the involvement of FOXO orthologue DAF-16 dependent longevity was obtained with PGEs (outer peel and inner peel) fed TJ356 worms. Overall, the results indicate the vital role of PGEs especially the extracts of outer peels in life-saving mechanisms of C. elegans by virtue of their antioxidant asset and life-prolonging effects via daf-16 dependent Insulin signaling pathway. See also Figure 1(Fig. 1). Leibniz Research Centre for Working Environment and Human Factors 2021-01-11 /pmc/articles/PMC7838930/ /pubmed/33510593 http://dx.doi.org/10.17179/excli2020-3011 Text en Copyright © 2021 Chaubey et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited.
spellingShingle Original Article
Chaubey, Mukesh G.
Chauhan, Anita P.
Chokshi, Pooja R.
Amin, Rahi S.
Patel, Stuti N.
Madamwar, Datta
Rastogi, Rajesh P.
Singh, Niraj Kumar
Therapeutic potential of bioactive compounds from Punica granatum extracts against aging and complicity of FOXO orthologue DAF-16 in Caenorhabditis elegans
title Therapeutic potential of bioactive compounds from Punica granatum extracts against aging and complicity of FOXO orthologue DAF-16 in Caenorhabditis elegans
title_full Therapeutic potential of bioactive compounds from Punica granatum extracts against aging and complicity of FOXO orthologue DAF-16 in Caenorhabditis elegans
title_fullStr Therapeutic potential of bioactive compounds from Punica granatum extracts against aging and complicity of FOXO orthologue DAF-16 in Caenorhabditis elegans
title_full_unstemmed Therapeutic potential of bioactive compounds from Punica granatum extracts against aging and complicity of FOXO orthologue DAF-16 in Caenorhabditis elegans
title_short Therapeutic potential of bioactive compounds from Punica granatum extracts against aging and complicity of FOXO orthologue DAF-16 in Caenorhabditis elegans
title_sort therapeutic potential of bioactive compounds from punica granatum extracts against aging and complicity of foxo orthologue daf-16 in caenorhabditis elegans
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838930/
https://www.ncbi.nlm.nih.gov/pubmed/33510593
http://dx.doi.org/10.17179/excli2020-3011
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