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Urinary 8-iso PGF(2α) and 2,3-dinor-8-iso PGF(2α) can be indexes of colitis-associated colorectal cancer in mice
Early diagnosis of colorectal cancer is needed to reduce the mortal consequence by cancer. Lipid mediators play critical role in progression of colitis and colitis-associated colon cancer (CAC) and some of their metabolites are excreted in urine. Here, we attempted to find novel biomarkers in urinar...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840041/ https://www.ncbi.nlm.nih.gov/pubmed/33503019 http://dx.doi.org/10.1371/journal.pone.0245292 |
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author | Miyazaki, Yusuke Nakamura, Tatsuro Takenouchi, Shinya Hayashi, Akane Omori, Keisuke Murata, Takahisa |
author_facet | Miyazaki, Yusuke Nakamura, Tatsuro Takenouchi, Shinya Hayashi, Akane Omori, Keisuke Murata, Takahisa |
author_sort | Miyazaki, Yusuke |
collection | PubMed |
description | Early diagnosis of colorectal cancer is needed to reduce the mortal consequence by cancer. Lipid mediators play critical role in progression of colitis and colitis-associated colon cancer (CAC) and some of their metabolites are excreted in urine. Here, we attempted to find novel biomarkers in urinary lipid metabolite of a murine model of CAC. Mice were received single administration of azoxymethane (AOM) and repeated administration of dextran sulfate sodium (DSS). Lipid metabolites in their urine was measured by liquid chromatography mass spectrometry and their colon was collected to perform morphological study. AOM and DSS caused inflammation and tumor formation in mouse colon. Liquid chromatography mass spectrometry-based comprehensive analysis of lipid metabolites showed that cyclooxygenase-mediated arachidonic acid (AA) metabolites, prostaglandins, and reactive oxygen species (ROS)-mediated AA metabolites, isoprostanes, were predominantly increased in the urine of tumor-bearing mice. Among that, urinary prostaglandin (PG)E(2) metabolite tetranor-PGEM and PGD(2) metabolite tetranor-PGDM were significantly increased in both of urine collected at the acute phase of colitis and the carcinogenesis phase. On the other hand, two F(2) isoprostanes (F(2)-IsoPs), 8-iso PGF(2α) and 2,3-dinor-8-iso PGF(2α), were significantly increased only in the carcinogenesis phase. Morphological study showed that infiltrated monocytes into tumor mass strongly expressed ROS generator NADPH (p22(phox)). These observations suggest that urinary 8-iso PGF(2α) and 2,3-dinor-8-iso PGF(2α) can be indexes of CAC. |
format | Online Article Text |
id | pubmed-7840041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78400412021-02-02 Urinary 8-iso PGF(2α) and 2,3-dinor-8-iso PGF(2α) can be indexes of colitis-associated colorectal cancer in mice Miyazaki, Yusuke Nakamura, Tatsuro Takenouchi, Shinya Hayashi, Akane Omori, Keisuke Murata, Takahisa PLoS One Research Article Early diagnosis of colorectal cancer is needed to reduce the mortal consequence by cancer. Lipid mediators play critical role in progression of colitis and colitis-associated colon cancer (CAC) and some of their metabolites are excreted in urine. Here, we attempted to find novel biomarkers in urinary lipid metabolite of a murine model of CAC. Mice were received single administration of azoxymethane (AOM) and repeated administration of dextran sulfate sodium (DSS). Lipid metabolites in their urine was measured by liquid chromatography mass spectrometry and their colon was collected to perform morphological study. AOM and DSS caused inflammation and tumor formation in mouse colon. Liquid chromatography mass spectrometry-based comprehensive analysis of lipid metabolites showed that cyclooxygenase-mediated arachidonic acid (AA) metabolites, prostaglandins, and reactive oxygen species (ROS)-mediated AA metabolites, isoprostanes, were predominantly increased in the urine of tumor-bearing mice. Among that, urinary prostaglandin (PG)E(2) metabolite tetranor-PGEM and PGD(2) metabolite tetranor-PGDM were significantly increased in both of urine collected at the acute phase of colitis and the carcinogenesis phase. On the other hand, two F(2) isoprostanes (F(2)-IsoPs), 8-iso PGF(2α) and 2,3-dinor-8-iso PGF(2α), were significantly increased only in the carcinogenesis phase. Morphological study showed that infiltrated monocytes into tumor mass strongly expressed ROS generator NADPH (p22(phox)). These observations suggest that urinary 8-iso PGF(2α) and 2,3-dinor-8-iso PGF(2α) can be indexes of CAC. Public Library of Science 2021-01-27 /pmc/articles/PMC7840041/ /pubmed/33503019 http://dx.doi.org/10.1371/journal.pone.0245292 Text en © 2021 Miyazaki et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Miyazaki, Yusuke Nakamura, Tatsuro Takenouchi, Shinya Hayashi, Akane Omori, Keisuke Murata, Takahisa Urinary 8-iso PGF(2α) and 2,3-dinor-8-iso PGF(2α) can be indexes of colitis-associated colorectal cancer in mice |
title | Urinary 8-iso PGF(2α) and 2,3-dinor-8-iso PGF(2α) can be indexes of colitis-associated colorectal cancer in mice |
title_full | Urinary 8-iso PGF(2α) and 2,3-dinor-8-iso PGF(2α) can be indexes of colitis-associated colorectal cancer in mice |
title_fullStr | Urinary 8-iso PGF(2α) and 2,3-dinor-8-iso PGF(2α) can be indexes of colitis-associated colorectal cancer in mice |
title_full_unstemmed | Urinary 8-iso PGF(2α) and 2,3-dinor-8-iso PGF(2α) can be indexes of colitis-associated colorectal cancer in mice |
title_short | Urinary 8-iso PGF(2α) and 2,3-dinor-8-iso PGF(2α) can be indexes of colitis-associated colorectal cancer in mice |
title_sort | urinary 8-iso pgf(2α) and 2,3-dinor-8-iso pgf(2α) can be indexes of colitis-associated colorectal cancer in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840041/ https://www.ncbi.nlm.nih.gov/pubmed/33503019 http://dx.doi.org/10.1371/journal.pone.0245292 |
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