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Expression of xenobiotic metabolising enzymes in lungs of horses with or without histological evidence of lower airway inflammation
Mild, moderate and severe equine asthma is a problem for equine welfare. The aetiology of the disease is not known in detail but is likely multi‐factorial. One important factor may be inhaled dust which carries harmful substances which may be bioactivated and thus can lead to local inflammation in t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840205/ https://www.ncbi.nlm.nih.gov/pubmed/32791560 http://dx.doi.org/10.1002/vms3.331 |
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author | Löfgren, Maria Larsson, Pia Lindberg, Ronny Hörnaeus, Katarina Tydén, Eva |
author_facet | Löfgren, Maria Larsson, Pia Lindberg, Ronny Hörnaeus, Katarina Tydén, Eva |
author_sort | Löfgren, Maria |
collection | PubMed |
description | Mild, moderate and severe equine asthma is a problem for equine welfare. The aetiology of the disease is not known in detail but is likely multi‐factorial. One important factor may be inhaled dust which carries harmful substances which may be bioactivated and thus can lead to local inflammation in the airways. The aim of this study was to investigate gene expression and protein localisation of cytochrome P450 (CYP) enzymes, superoxide dismutase and glutathione‐S‐transferases (GST) involved in bioactivation and detoxification of harmful substances in lungs of horses with or without histological evidence of lower airway inflammation. Significantly lower gene expression of CYP2A13 and GSTM1 was observed in lungs from horses with histological evidence of lower airway inflammation compared with horses without. A higher expression, although not significant, was found for CYP1A1 in horses with histological evidence of lower airway inflammation. There were no differences in gene expression of GSTP1 and SOD3. The proteins were localised in the respiratory epithelium which is of relevance as a defence to local exposure of inhaled harmful substances. In conclusion, our study reports differential gene expression of enzymes involved in bioactivation and detoxification of foreign substances in the lungs of horses with histological evidence of lower airway inflammation compared with horses without. |
format | Online Article Text |
id | pubmed-7840205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78402052021-02-04 Expression of xenobiotic metabolising enzymes in lungs of horses with or without histological evidence of lower airway inflammation Löfgren, Maria Larsson, Pia Lindberg, Ronny Hörnaeus, Katarina Tydén, Eva Vet Med Sci Original Articles Mild, moderate and severe equine asthma is a problem for equine welfare. The aetiology of the disease is not known in detail but is likely multi‐factorial. One important factor may be inhaled dust which carries harmful substances which may be bioactivated and thus can lead to local inflammation in the airways. The aim of this study was to investigate gene expression and protein localisation of cytochrome P450 (CYP) enzymes, superoxide dismutase and glutathione‐S‐transferases (GST) involved in bioactivation and detoxification of harmful substances in lungs of horses with or without histological evidence of lower airway inflammation. Significantly lower gene expression of CYP2A13 and GSTM1 was observed in lungs from horses with histological evidence of lower airway inflammation compared with horses without. A higher expression, although not significant, was found for CYP1A1 in horses with histological evidence of lower airway inflammation. There were no differences in gene expression of GSTP1 and SOD3. The proteins were localised in the respiratory epithelium which is of relevance as a defence to local exposure of inhaled harmful substances. In conclusion, our study reports differential gene expression of enzymes involved in bioactivation and detoxification of foreign substances in the lungs of horses with histological evidence of lower airway inflammation compared with horses without. John Wiley and Sons Inc. 2020-08-13 /pmc/articles/PMC7840205/ /pubmed/32791560 http://dx.doi.org/10.1002/vms3.331 Text en © 2020 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Löfgren, Maria Larsson, Pia Lindberg, Ronny Hörnaeus, Katarina Tydén, Eva Expression of xenobiotic metabolising enzymes in lungs of horses with or without histological evidence of lower airway inflammation |
title | Expression of xenobiotic metabolising enzymes in lungs of horses with or without histological evidence of lower airway inflammation |
title_full | Expression of xenobiotic metabolising enzymes in lungs of horses with or without histological evidence of lower airway inflammation |
title_fullStr | Expression of xenobiotic metabolising enzymes in lungs of horses with or without histological evidence of lower airway inflammation |
title_full_unstemmed | Expression of xenobiotic metabolising enzymes in lungs of horses with or without histological evidence of lower airway inflammation |
title_short | Expression of xenobiotic metabolising enzymes in lungs of horses with or without histological evidence of lower airway inflammation |
title_sort | expression of xenobiotic metabolising enzymes in lungs of horses with or without histological evidence of lower airway inflammation |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840205/ https://www.ncbi.nlm.nih.gov/pubmed/32791560 http://dx.doi.org/10.1002/vms3.331 |
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