Cargando…
MicroRNA-377 Downregulates Bcl-xL and Increases Apoptosis in Hepatocellular Carcinoma Cells
Aberrantly expressed microRNAs (miRNAs/miRs) and their role in cancer development have recently gained more attention. However, the potential role of miRNAs in hepatocellular carcinoma (HCC) remains largely unknown. In this study, we demonstrated that miR-377 was markedly downregulated in HCC cell l...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cognizant Communication Corporation
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840731/ https://www.ncbi.nlm.nih.gov/pubmed/28081730 http://dx.doi.org/10.3727/096504016X14719078133168 |
_version_ | 1783643638422568960 |
---|---|
author | Ge, Hongyan Zou, Di Wang, Yingshu Jiang, Hao Wang, Liping |
author_facet | Ge, Hongyan Zou, Di Wang, Yingshu Jiang, Hao Wang, Liping |
author_sort | Ge, Hongyan |
collection | PubMed |
description | Aberrantly expressed microRNAs (miRNAs/miRs) and their role in cancer development have recently gained more attention. However, the potential role of miRNAs in hepatocellular carcinoma (HCC) remains largely unknown. In this study, we demonstrated that miR-377 was markedly downregulated in HCC cell lines and primary human HCC tissues. The decreased expression of miR-377 contributes to the upregulation of Bcl-xL expression by targeting its 3′-untranslated region (3′-UTR). Functionally, knockdown of miR-377 noticeably increased HCC cell growth and colony formation and inhibited apoptosis. In contrast, overexpression of miR-377 suppressed cell proliferation and increased apoptosis. This study provides new insights for the use of miR-377 as a potential molecular target in HCC therapy. |
format | Online Article Text |
id | pubmed-7840731 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Cognizant Communication Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-78407312021-02-16 MicroRNA-377 Downregulates Bcl-xL and Increases Apoptosis in Hepatocellular Carcinoma Cells Ge, Hongyan Zou, Di Wang, Yingshu Jiang, Hao Wang, Liping Oncol Res Article Aberrantly expressed microRNAs (miRNAs/miRs) and their role in cancer development have recently gained more attention. However, the potential role of miRNAs in hepatocellular carcinoma (HCC) remains largely unknown. In this study, we demonstrated that miR-377 was markedly downregulated in HCC cell lines and primary human HCC tissues. The decreased expression of miR-377 contributes to the upregulation of Bcl-xL expression by targeting its 3′-untranslated region (3′-UTR). Functionally, knockdown of miR-377 noticeably increased HCC cell growth and colony formation and inhibited apoptosis. In contrast, overexpression of miR-377 suppressed cell proliferation and increased apoptosis. This study provides new insights for the use of miR-377 as a potential molecular target in HCC therapy. Cognizant Communication Corporation 2017-01-02 /pmc/articles/PMC7840731/ /pubmed/28081730 http://dx.doi.org/10.3727/096504016X14719078133168 Text en Copyright © 2017 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License. |
spellingShingle | Article Ge, Hongyan Zou, Di Wang, Yingshu Jiang, Hao Wang, Liping MicroRNA-377 Downregulates Bcl-xL and Increases Apoptosis in Hepatocellular Carcinoma Cells |
title | MicroRNA-377 Downregulates Bcl-xL and Increases Apoptosis in Hepatocellular Carcinoma Cells |
title_full | MicroRNA-377 Downregulates Bcl-xL and Increases Apoptosis in Hepatocellular Carcinoma Cells |
title_fullStr | MicroRNA-377 Downregulates Bcl-xL and Increases Apoptosis in Hepatocellular Carcinoma Cells |
title_full_unstemmed | MicroRNA-377 Downregulates Bcl-xL and Increases Apoptosis in Hepatocellular Carcinoma Cells |
title_short | MicroRNA-377 Downregulates Bcl-xL and Increases Apoptosis in Hepatocellular Carcinoma Cells |
title_sort | microrna-377 downregulates bcl-xl and increases apoptosis in hepatocellular carcinoma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840731/ https://www.ncbi.nlm.nih.gov/pubmed/28081730 http://dx.doi.org/10.3727/096504016X14719078133168 |
work_keys_str_mv | AT gehongyan microrna377downregulatesbclxlandincreasesapoptosisinhepatocellularcarcinomacells AT zoudi microrna377downregulatesbclxlandincreasesapoptosisinhepatocellularcarcinomacells AT wangyingshu microrna377downregulatesbclxlandincreasesapoptosisinhepatocellularcarcinomacells AT jianghao microrna377downregulatesbclxlandincreasesapoptosisinhepatocellularcarcinomacells AT wangliping microrna377downregulatesbclxlandincreasesapoptosisinhepatocellularcarcinomacells |