Cargando…
Overexpression of MicroRNA-27b Inhibits Proliferation, Migration, and Invasion via Suppression of MET Expression
MicroRNA-27b (miR-27b) was recently found to be significantly downregulated in different human cancers. However, evidence of the function of miR-27b in non-small cell lung cancer (NSCLC) remains limited. In this study, we aimed to investigate novel miR-27b-mediated targets or signaling pathways asso...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cognizant Communication Corporation
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840835/ https://www.ncbi.nlm.nih.gov/pubmed/28081743 http://dx.doi.org/10.3727/096504016X14732772150505 |
_version_ | 1783643662866972672 |
---|---|
author | Zhou, Hui Liu, Yanglin Xiao, Ling Hu, Zhengmao Xia, Kun |
author_facet | Zhou, Hui Liu, Yanglin Xiao, Ling Hu, Zhengmao Xia, Kun |
author_sort | Zhou, Hui |
collection | PubMed |
description | MicroRNA-27b (miR-27b) was recently found to be significantly downregulated in different human cancers. However, evidence of the function of miR-27b in non-small cell lung cancer (NSCLC) remains limited. In this study, we aimed to investigate novel miR-27b-mediated targets or signaling pathways associated with the tumorigenesis and metastasis of NSCLC. Real-time (RT) PCR was performed to examine miR-27b expression in NSCLC specimens. MTT assay, wound-healing assay, and Transwell assay were used to determine cell proliferation, migration, and invasion. Our data indicated that the miR-27b levels were significantly decreased in NSCLC specimens and cell lines (SK-MES-1, H358, H460, A549, and H1229) when compared to matched normal adjacent tissues and normal human lung epithelial cell lines, respectively. Restoration of miR-27b significantly inhibited the proliferation, migration, and invasion of A549 cells. We then conducted in silico analysis and luciferase reporter gene assay and identified MET, a receptor tyrosine kinase, as a direct target of miR-27b in NSCLC cells. Moreover, overexpression of MET rescued the suppressive effect of miR-27b on the proliferation, migration, and invasion of A549 cells, suggesting that MET acts as a downstream effecter of miR-27b in NSCLC cells. In summary, our study identified a novel miR-27b/MET signaling pathway involved in the cell proliferation, migration, and invasion of NSCLC, and identification of miR-27b-mediated novel signaling pathways may help reveal the molecular mechanism underlying the development and malignant progression of this disease. |
format | Online Article Text |
id | pubmed-7840835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Cognizant Communication Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-78408352021-02-16 Overexpression of MicroRNA-27b Inhibits Proliferation, Migration, and Invasion via Suppression of MET Expression Zhou, Hui Liu, Yanglin Xiao, Ling Hu, Zhengmao Xia, Kun Oncol Res Article MicroRNA-27b (miR-27b) was recently found to be significantly downregulated in different human cancers. However, evidence of the function of miR-27b in non-small cell lung cancer (NSCLC) remains limited. In this study, we aimed to investigate novel miR-27b-mediated targets or signaling pathways associated with the tumorigenesis and metastasis of NSCLC. Real-time (RT) PCR was performed to examine miR-27b expression in NSCLC specimens. MTT assay, wound-healing assay, and Transwell assay were used to determine cell proliferation, migration, and invasion. Our data indicated that the miR-27b levels were significantly decreased in NSCLC specimens and cell lines (SK-MES-1, H358, H460, A549, and H1229) when compared to matched normal adjacent tissues and normal human lung epithelial cell lines, respectively. Restoration of miR-27b significantly inhibited the proliferation, migration, and invasion of A549 cells. We then conducted in silico analysis and luciferase reporter gene assay and identified MET, a receptor tyrosine kinase, as a direct target of miR-27b in NSCLC cells. Moreover, overexpression of MET rescued the suppressive effect of miR-27b on the proliferation, migration, and invasion of A549 cells, suggesting that MET acts as a downstream effecter of miR-27b in NSCLC cells. In summary, our study identified a novel miR-27b/MET signaling pathway involved in the cell proliferation, migration, and invasion of NSCLC, and identification of miR-27b-mediated novel signaling pathways may help reveal the molecular mechanism underlying the development and malignant progression of this disease. Cognizant Communication Corporation 2017-01-02 /pmc/articles/PMC7840835/ /pubmed/28081743 http://dx.doi.org/10.3727/096504016X14732772150505 Text en Copyright © 2017 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License. |
spellingShingle | Article Zhou, Hui Liu, Yanglin Xiao, Ling Hu, Zhengmao Xia, Kun Overexpression of MicroRNA-27b Inhibits Proliferation, Migration, and Invasion via Suppression of MET Expression |
title | Overexpression of MicroRNA-27b Inhibits Proliferation, Migration, and Invasion via Suppression of MET Expression |
title_full | Overexpression of MicroRNA-27b Inhibits Proliferation, Migration, and Invasion via Suppression of MET Expression |
title_fullStr | Overexpression of MicroRNA-27b Inhibits Proliferation, Migration, and Invasion via Suppression of MET Expression |
title_full_unstemmed | Overexpression of MicroRNA-27b Inhibits Proliferation, Migration, and Invasion via Suppression of MET Expression |
title_short | Overexpression of MicroRNA-27b Inhibits Proliferation, Migration, and Invasion via Suppression of MET Expression |
title_sort | overexpression of microrna-27b inhibits proliferation, migration, and invasion via suppression of met expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840835/ https://www.ncbi.nlm.nih.gov/pubmed/28081743 http://dx.doi.org/10.3727/096504016X14732772150505 |
work_keys_str_mv | AT zhouhui overexpressionofmicrorna27binhibitsproliferationmigrationandinvasionviasuppressionofmetexpression AT liuyanglin overexpressionofmicrorna27binhibitsproliferationmigrationandinvasionviasuppressionofmetexpression AT xiaoling overexpressionofmicrorna27binhibitsproliferationmigrationandinvasionviasuppressionofmetexpression AT huzhengmao overexpressionofmicrorna27binhibitsproliferationmigrationandinvasionviasuppressionofmetexpression AT xiakun overexpressionofmicrorna27binhibitsproliferationmigrationandinvasionviasuppressionofmetexpression |