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Downregulation of miR-222 Induces Apoptosis and Cellular Migration in Adenoid Cystic Carcinoma Cells

Previous studies have shown that miR-222 targets the p53 upregulated modulator of apoptosis (PUMA) to regulate cell biological behavior in some human malignancies. We hypothesized that there was a negative regulation, which might induce apoptosis, between miR-222 and PUMA in adenoid cystic carcinoma...

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Autores principales: Zhou, Ziliang, Zhou, Lijie, Jiang, Fangfang, Zeng, Binghui, Wei, Changbo, Zhao, Wei, Yu, Dongsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840837/
https://www.ncbi.nlm.nih.gov/pubmed/28277192
http://dx.doi.org/10.3727/096504016X14732772150460
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author Zhou, Ziliang
Zhou, Lijie
Jiang, Fangfang
Zeng, Binghui
Wei, Changbo
Zhao, Wei
Yu, Dongsheng
author_facet Zhou, Ziliang
Zhou, Lijie
Jiang, Fangfang
Zeng, Binghui
Wei, Changbo
Zhao, Wei
Yu, Dongsheng
author_sort Zhou, Ziliang
collection PubMed
description Previous studies have shown that miR-222 targets the p53 upregulated modulator of apoptosis (PUMA) to regulate cell biological behavior in some human malignancies. We hypothesized that there was a negative regulation, which might induce apoptosis, between miR-222 and PUMA in adenoid cystic carcinoma (ACC). In this study, the expression levels of miR-222 and the PUMA gene after transfection with antisense miR-222 (As-miR-222) were evaluated by RT-PCR and Western blot assays. Cell proliferation and migratory abilities were detected by CCK-8 and Transwell assays. Cell cycle and apoptosis were analyzed by flow cytometry. Our results showed that, when compared with the control and scramble-transfected groups, the expression of miR-222 in the As-miR-222 group was downregulated, while the expression of PUMA at both mRNA and protein levels was upregulated, cell proliferation and migratory abilities were inhibited, and apoptosis was increased. Our results suggested that As-miR-222 transfection could upregulate the expression of PUMA to induce apoptosis in ACC, providing a new concept for the treatment of ACC.
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spelling pubmed-78408372021-02-16 Downregulation of miR-222 Induces Apoptosis and Cellular Migration in Adenoid Cystic Carcinoma Cells Zhou, Ziliang Zhou, Lijie Jiang, Fangfang Zeng, Binghui Wei, Changbo Zhao, Wei Yu, Dongsheng Oncol Res Article Previous studies have shown that miR-222 targets the p53 upregulated modulator of apoptosis (PUMA) to regulate cell biological behavior in some human malignancies. We hypothesized that there was a negative regulation, which might induce apoptosis, between miR-222 and PUMA in adenoid cystic carcinoma (ACC). In this study, the expression levels of miR-222 and the PUMA gene after transfection with antisense miR-222 (As-miR-222) were evaluated by RT-PCR and Western blot assays. Cell proliferation and migratory abilities were detected by CCK-8 and Transwell assays. Cell cycle and apoptosis were analyzed by flow cytometry. Our results showed that, when compared with the control and scramble-transfected groups, the expression of miR-222 in the As-miR-222 group was downregulated, while the expression of PUMA at both mRNA and protein levels was upregulated, cell proliferation and migratory abilities were inhibited, and apoptosis was increased. Our results suggested that As-miR-222 transfection could upregulate the expression of PUMA to induce apoptosis in ACC, providing a new concept for the treatment of ACC. Cognizant Communication Corporation 2017-01-26 /pmc/articles/PMC7840837/ /pubmed/28277192 http://dx.doi.org/10.3727/096504016X14732772150460 Text en Copyright © 2017 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Zhou, Ziliang
Zhou, Lijie
Jiang, Fangfang
Zeng, Binghui
Wei, Changbo
Zhao, Wei
Yu, Dongsheng
Downregulation of miR-222 Induces Apoptosis and Cellular Migration in Adenoid Cystic Carcinoma Cells
title Downregulation of miR-222 Induces Apoptosis and Cellular Migration in Adenoid Cystic Carcinoma Cells
title_full Downregulation of miR-222 Induces Apoptosis and Cellular Migration in Adenoid Cystic Carcinoma Cells
title_fullStr Downregulation of miR-222 Induces Apoptosis and Cellular Migration in Adenoid Cystic Carcinoma Cells
title_full_unstemmed Downregulation of miR-222 Induces Apoptosis and Cellular Migration in Adenoid Cystic Carcinoma Cells
title_short Downregulation of miR-222 Induces Apoptosis and Cellular Migration in Adenoid Cystic Carcinoma Cells
title_sort downregulation of mir-222 induces apoptosis and cellular migration in adenoid cystic carcinoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840837/
https://www.ncbi.nlm.nih.gov/pubmed/28277192
http://dx.doi.org/10.3727/096504016X14732772150460
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