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Silencing of Ribosomal Protein L34 (RPL34) Inhibits the Proliferation and Invasion of Esophageal Cancer Cells

Ribosomal protein L34 (RPL34) belongs to the L34E family of ribosomal proteins and contains a zinc finger motif. Aberrant expression of RPL34 has been reported in several human malignancies. However, the precise role and potential underlying mechanisms of RPL34 in human esophageal cancer remain larg...

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Autores principales: Fan, Huijie, Li, Jing, Jia, Yongxu, Wu, Jingjing, Yuan, Long, Li, Mingjun, Wei, Jiangqi, Xu, Benling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840969/
https://www.ncbi.nlm.nih.gov/pubmed/28109079
http://dx.doi.org/10.3727/096504016X14830466773541
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author Fan, Huijie
Li, Jing
Jia, Yongxu
Wu, Jingjing
Yuan, Long
Li, Mingjun
Wei, Jiangqi
Xu, Benling
author_facet Fan, Huijie
Li, Jing
Jia, Yongxu
Wu, Jingjing
Yuan, Long
Li, Mingjun
Wei, Jiangqi
Xu, Benling
author_sort Fan, Huijie
collection PubMed
description Ribosomal protein L34 (RPL34) belongs to the L34E family of ribosomal proteins and contains a zinc finger motif. Aberrant expression of RPL34 has been reported in several human malignancies. However, the precise role and potential underlying mechanisms of RPL34 in human esophageal cancer remain largely unknown. Thus, the objective of this study was to investigate the role of RPL34 in esophageal cancer progression. Our results showed that the expression of RPL34 at both the mRNA and protein levels was frequently upregulated in esophageal cancer cell lines. Knockdown of RPL34 efficiently inhibited esophageal cancer cell proliferation, migration, and invasion in vitro. Mechanistically, knockdown of RPL34 significantly downregulated the protein expression level of p-PI3K and p-Akt in esophageal cancer cells. Finally, knockdown of RPL34 attenuated tumor growth in nude mice. In conclusion, our study revealed that RPL34 functions as an oncogene that modulates the proliferation and metastasis of esophageal cancer cells, in part, by the inactivation of the PI3K/Akt signaling pathway. Thus, these findings suggest that RPL34 may serve as a potential therapeutic target for the treatment of esophageal cancer.
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spelling pubmed-78409692021-02-16 Silencing of Ribosomal Protein L34 (RPL34) Inhibits the Proliferation and Invasion of Esophageal Cancer Cells Fan, Huijie Li, Jing Jia, Yongxu Wu, Jingjing Yuan, Long Li, Mingjun Wei, Jiangqi Xu, Benling Oncol Res Article Ribosomal protein L34 (RPL34) belongs to the L34E family of ribosomal proteins and contains a zinc finger motif. Aberrant expression of RPL34 has been reported in several human malignancies. However, the precise role and potential underlying mechanisms of RPL34 in human esophageal cancer remain largely unknown. Thus, the objective of this study was to investigate the role of RPL34 in esophageal cancer progression. Our results showed that the expression of RPL34 at both the mRNA and protein levels was frequently upregulated in esophageal cancer cell lines. Knockdown of RPL34 efficiently inhibited esophageal cancer cell proliferation, migration, and invasion in vitro. Mechanistically, knockdown of RPL34 significantly downregulated the protein expression level of p-PI3K and p-Akt in esophageal cancer cells. Finally, knockdown of RPL34 attenuated tumor growth in nude mice. In conclusion, our study revealed that RPL34 functions as an oncogene that modulates the proliferation and metastasis of esophageal cancer cells, in part, by the inactivation of the PI3K/Akt signaling pathway. Thus, these findings suggest that RPL34 may serve as a potential therapeutic target for the treatment of esophageal cancer. Cognizant Communication Corporation 2017-08-07 /pmc/articles/PMC7840969/ /pubmed/28109079 http://dx.doi.org/10.3727/096504016X14830466773541 Text en Copyright © 2017 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Fan, Huijie
Li, Jing
Jia, Yongxu
Wu, Jingjing
Yuan, Long
Li, Mingjun
Wei, Jiangqi
Xu, Benling
Silencing of Ribosomal Protein L34 (RPL34) Inhibits the Proliferation and Invasion of Esophageal Cancer Cells
title Silencing of Ribosomal Protein L34 (RPL34) Inhibits the Proliferation and Invasion of Esophageal Cancer Cells
title_full Silencing of Ribosomal Protein L34 (RPL34) Inhibits the Proliferation and Invasion of Esophageal Cancer Cells
title_fullStr Silencing of Ribosomal Protein L34 (RPL34) Inhibits the Proliferation and Invasion of Esophageal Cancer Cells
title_full_unstemmed Silencing of Ribosomal Protein L34 (RPL34) Inhibits the Proliferation and Invasion of Esophageal Cancer Cells
title_short Silencing of Ribosomal Protein L34 (RPL34) Inhibits the Proliferation and Invasion of Esophageal Cancer Cells
title_sort silencing of ribosomal protein l34 (rpl34) inhibits the proliferation and invasion of esophageal cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840969/
https://www.ncbi.nlm.nih.gov/pubmed/28109079
http://dx.doi.org/10.3727/096504016X14830466773541
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