Cargando…

Knockdown of FOXK1 Suppresses Proliferation, Migration, and Invasion in Prostate Cancer Cells

Forkhead box K1 (FOXK1) is a member of the FOX transcription factor family and plays an important role in the development of several tumors. However, the role of FOXK1 in the progression of prostate cancer remains unknown. Thus, the objectives of this study were to detect the expression of FOXK1 in...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Fang, Xiong, Wei, Dou, Ke, Ran, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841013/
https://www.ncbi.nlm.nih.gov/pubmed/28267429
http://dx.doi.org/10.3727/096504017X14871164924588
_version_ 1783643706001195008
author Chen, Fang
Xiong, Wei
Dou, Ke
Ran, Qing
author_facet Chen, Fang
Xiong, Wei
Dou, Ke
Ran, Qing
author_sort Chen, Fang
collection PubMed
description Forkhead box K1 (FOXK1) is a member of the FOX transcription factor family and plays an important role in the development of several tumors. However, the role of FOXK1 in the progression of prostate cancer remains unknown. Thus, the objectives of this study were to detect the expression of FOXK1 in prostate cancer and to examine its role in prostate cancer cells. We found that the expression of FOXK1 at both the mRNA and protein levels was significantly upregulated in human prostate cancer cell lines. In addition, the downregulation of FOXK1 obviously inhibited the cell proliferation of prostate cancer cells in vitro and attenuated tumor growth in a xenograft model in vivo. Furthermore, knockdown of FOXK1 suppressed the migration and invasion of prostate cancer cells, and prevented the EMT phenotype through upregulating the expression of E-cadherin, as well as downregulating the expression of N-cadherin in prostate cancer cells. Mechanistically, knockdown of FOXK1 efficiently downregulated the expression levels of β-catenin, c-myc, and cyclin D1 in PC-3 cells. Overall, our results demonstrated that knockdown of FOXK1 inhibited the proliferation and metastasis of prostate cancer, at least in part, through suppressing the Wnt/β-catenin signaling pathway. Therefore, these results suggest that FOXK1 may be a potential therapeutic target for human prostate cancer.
format Online
Article
Text
id pubmed-7841013
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Cognizant Communication Corporation
record_format MEDLINE/PubMed
spelling pubmed-78410132021-02-16 Knockdown of FOXK1 Suppresses Proliferation, Migration, and Invasion in Prostate Cancer Cells Chen, Fang Xiong, Wei Dou, Ke Ran, Qing Oncol Res Article Forkhead box K1 (FOXK1) is a member of the FOX transcription factor family and plays an important role in the development of several tumors. However, the role of FOXK1 in the progression of prostate cancer remains unknown. Thus, the objectives of this study were to detect the expression of FOXK1 in prostate cancer and to examine its role in prostate cancer cells. We found that the expression of FOXK1 at both the mRNA and protein levels was significantly upregulated in human prostate cancer cell lines. In addition, the downregulation of FOXK1 obviously inhibited the cell proliferation of prostate cancer cells in vitro and attenuated tumor growth in a xenograft model in vivo. Furthermore, knockdown of FOXK1 suppressed the migration and invasion of prostate cancer cells, and prevented the EMT phenotype through upregulating the expression of E-cadherin, as well as downregulating the expression of N-cadherin in prostate cancer cells. Mechanistically, knockdown of FOXK1 efficiently downregulated the expression levels of β-catenin, c-myc, and cyclin D1 in PC-3 cells. Overall, our results demonstrated that knockdown of FOXK1 inhibited the proliferation and metastasis of prostate cancer, at least in part, through suppressing the Wnt/β-catenin signaling pathway. Therefore, these results suggest that FOXK1 may be a potential therapeutic target for human prostate cancer. Cognizant Communication Corporation 2017-09-21 /pmc/articles/PMC7841013/ /pubmed/28267429 http://dx.doi.org/10.3727/096504017X14871164924588 Text en Copyright © 2017 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Chen, Fang
Xiong, Wei
Dou, Ke
Ran, Qing
Knockdown of FOXK1 Suppresses Proliferation, Migration, and Invasion in Prostate Cancer Cells
title Knockdown of FOXK1 Suppresses Proliferation, Migration, and Invasion in Prostate Cancer Cells
title_full Knockdown of FOXK1 Suppresses Proliferation, Migration, and Invasion in Prostate Cancer Cells
title_fullStr Knockdown of FOXK1 Suppresses Proliferation, Migration, and Invasion in Prostate Cancer Cells
title_full_unstemmed Knockdown of FOXK1 Suppresses Proliferation, Migration, and Invasion in Prostate Cancer Cells
title_short Knockdown of FOXK1 Suppresses Proliferation, Migration, and Invasion in Prostate Cancer Cells
title_sort knockdown of foxk1 suppresses proliferation, migration, and invasion in prostate cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841013/
https://www.ncbi.nlm.nih.gov/pubmed/28267429
http://dx.doi.org/10.3727/096504017X14871164924588
work_keys_str_mv AT chenfang knockdownoffoxk1suppressesproliferationmigrationandinvasioninprostatecancercells
AT xiongwei knockdownoffoxk1suppressesproliferationmigrationandinvasioninprostatecancercells
AT douke knockdownoffoxk1suppressesproliferationmigrationandinvasioninprostatecancercells
AT ranqing knockdownoffoxk1suppressesproliferationmigrationandinvasioninprostatecancercells