Cargando…

MicroRNA-98 Plays a Suppressive Role in Non-Small Cell Lung Cancer Through Inhibition of SALL4 Protein Expression

MicroRNAs (miRs) have been demonstrated to be significantly associated with the development and progression of non-small cell lung cancer (NSCLC). However, the underlying mechanism of miR-98 in mediating the malignant phenotypes of NSCLC cells remains obscure. In this study, we found that miR-98 was...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Wenliang, Xiao, Peng, Wu, Han, Wang, Li, Kong, Demiao, Yu, Fenglei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841028/
https://www.ncbi.nlm.nih.gov/pubmed/27938506
http://dx.doi.org/10.3727/096504016X14791726591124
_version_ 1783643709762437120
author Liu, Wenliang
Xiao, Peng
Wu, Han
Wang, Li
Kong, Demiao
Yu, Fenglei
author_facet Liu, Wenliang
Xiao, Peng
Wu, Han
Wang, Li
Kong, Demiao
Yu, Fenglei
author_sort Liu, Wenliang
collection PubMed
description MicroRNAs (miRs) have been demonstrated to be significantly associated with the development and progression of non-small cell lung cancer (NSCLC). However, the underlying mechanism of miR-98 in mediating the malignant phenotypes of NSCLC cells remains obscure. In this study, we found that miR-98 was significantly downregulated in NSCLC tissues compared to nontumor lung tissues. Downregulation of miR-98 was significantly associated with poor differentiation and advanced clinical stage. Restoration of miR-98 expression significantly decreased the proliferation, migration, and invasion of NSCLC A549 and H1229 cells. SALL4 was identified as a target gene of miR-98, and the protein expression of SALL4 was negatively regulated by miR-98 in NSCLC A549 and H1229 cells. Overexpression of SALL4 promoted A549 and H1229 cell proliferation, migration, and invasion and reversed the suppressive effects of miR-98 on the malignant phenotypes of A549 and H1229 cells. Moreover, SALL4 was found to be significantly upregulated in NSCLC tissues compared to the nontumor lung tissues. We then observed an inverse correlation between the miR-98 and SALL4 levels in NSCLC tissues. In vivo study revealed that miR-98 overexpression suppressed NSCLC growth. In summary, we demonstrate that miR-98 acts as a tumor suppressor in NSCLC cells by inhibiting the protein expression of its target gene SALL4. Therefore, our study highlights the importance of the miR-98/SALL4 axis in NSCLC.
format Online
Article
Text
id pubmed-7841028
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Cognizant Communication Corporation
record_format MEDLINE/PubMed
spelling pubmed-78410282021-02-16 MicroRNA-98 Plays a Suppressive Role in Non-Small Cell Lung Cancer Through Inhibition of SALL4 Protein Expression Liu, Wenliang Xiao, Peng Wu, Han Wang, Li Kong, Demiao Yu, Fenglei Oncol Res Article MicroRNAs (miRs) have been demonstrated to be significantly associated with the development and progression of non-small cell lung cancer (NSCLC). However, the underlying mechanism of miR-98 in mediating the malignant phenotypes of NSCLC cells remains obscure. In this study, we found that miR-98 was significantly downregulated in NSCLC tissues compared to nontumor lung tissues. Downregulation of miR-98 was significantly associated with poor differentiation and advanced clinical stage. Restoration of miR-98 expression significantly decreased the proliferation, migration, and invasion of NSCLC A549 and H1229 cells. SALL4 was identified as a target gene of miR-98, and the protein expression of SALL4 was negatively regulated by miR-98 in NSCLC A549 and H1229 cells. Overexpression of SALL4 promoted A549 and H1229 cell proliferation, migration, and invasion and reversed the suppressive effects of miR-98 on the malignant phenotypes of A549 and H1229 cells. Moreover, SALL4 was found to be significantly upregulated in NSCLC tissues compared to the nontumor lung tissues. We then observed an inverse correlation between the miR-98 and SALL4 levels in NSCLC tissues. In vivo study revealed that miR-98 overexpression suppressed NSCLC growth. In summary, we demonstrate that miR-98 acts as a tumor suppressor in NSCLC cells by inhibiting the protein expression of its target gene SALL4. Therefore, our study highlights the importance of the miR-98/SALL4 axis in NSCLC. Cognizant Communication Corporation 2017-07-05 /pmc/articles/PMC7841028/ /pubmed/27938506 http://dx.doi.org/10.3727/096504016X14791726591124 Text en Copyright © 2017 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Liu, Wenliang
Xiao, Peng
Wu, Han
Wang, Li
Kong, Demiao
Yu, Fenglei
MicroRNA-98 Plays a Suppressive Role in Non-Small Cell Lung Cancer Through Inhibition of SALL4 Protein Expression
title MicroRNA-98 Plays a Suppressive Role in Non-Small Cell Lung Cancer Through Inhibition of SALL4 Protein Expression
title_full MicroRNA-98 Plays a Suppressive Role in Non-Small Cell Lung Cancer Through Inhibition of SALL4 Protein Expression
title_fullStr MicroRNA-98 Plays a Suppressive Role in Non-Small Cell Lung Cancer Through Inhibition of SALL4 Protein Expression
title_full_unstemmed MicroRNA-98 Plays a Suppressive Role in Non-Small Cell Lung Cancer Through Inhibition of SALL4 Protein Expression
title_short MicroRNA-98 Plays a Suppressive Role in Non-Small Cell Lung Cancer Through Inhibition of SALL4 Protein Expression
title_sort microrna-98 plays a suppressive role in non-small cell lung cancer through inhibition of sall4 protein expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841028/
https://www.ncbi.nlm.nih.gov/pubmed/27938506
http://dx.doi.org/10.3727/096504016X14791726591124
work_keys_str_mv AT liuwenliang microrna98playsasuppressiveroleinnonsmallcelllungcancerthroughinhibitionofsall4proteinexpression
AT xiaopeng microrna98playsasuppressiveroleinnonsmallcelllungcancerthroughinhibitionofsall4proteinexpression
AT wuhan microrna98playsasuppressiveroleinnonsmallcelllungcancerthroughinhibitionofsall4proteinexpression
AT wangli microrna98playsasuppressiveroleinnonsmallcelllungcancerthroughinhibitionofsall4proteinexpression
AT kongdemiao microrna98playsasuppressiveroleinnonsmallcelllungcancerthroughinhibitionofsall4proteinexpression
AT yufenglei microrna98playsasuppressiveroleinnonsmallcelllungcancerthroughinhibitionofsall4proteinexpression