Cargando…

MicroRNA-342-3p Inhibits the Proliferation, Migration, and Invasion of Osteosarcoma Cells by Targeting Astrocyte-Elevated Gene-1 (AEG-1)

Recent studies suggest that microRNAs (miRNAs) are critical regulators in many types of cancer, including osteosarcoma. miR-342-3p has emerged as an important cancer-related miRNA in several types of cancers. However, the functional significance of miR-342-3p in osteosarcoma is unknown. The aims of...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Shaokun, Liu, Lidi, Lv, Zhenshan, Li, Qiao, Gong, Weiquan, Wu, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841055/
https://www.ncbi.nlm.nih.gov/pubmed/28276315
http://dx.doi.org/10.3727/096504017X14886485417426
_version_ 1783643716530995200
author Zhang, Shaokun
Liu, Lidi
Lv, Zhenshan
Li, Qiao
Gong, Weiquan
Wu, Hong
author_facet Zhang, Shaokun
Liu, Lidi
Lv, Zhenshan
Li, Qiao
Gong, Weiquan
Wu, Hong
author_sort Zhang, Shaokun
collection PubMed
description Recent studies suggest that microRNAs (miRNAs) are critical regulators in many types of cancer, including osteosarcoma. miR-342-3p has emerged as an important cancer-related miRNA in several types of cancers. However, the functional significance of miR-342-3p in osteosarcoma is unknown. The aims of this study were to investigate whether miR-342-3p is dysregulated in osteosarcoma and to explore the biological function of miR-342-3p in regulating cellular processes of osteosarcoma cells. We found that miR-342-3p expression was significantly decreased in osteosarcoma tissues and cell lines. Overexpression of miR-342-3p inhibits the proliferation, migration, and invasion of osteosarcoma cells. In contrast, the inhibition of miR-342-3p exhibited the opposite effect. Astrocyte-elevated gene-1 (AEG-1) was identified as one of the target genes of miR-342-3p in osteosarcoma cells by bioinformatics analysis, dual-luciferase reporter assay, real-time quantitative polymerase chain reaction, and Western blot analysis. Overexpression of miR-342-3p also inhibited the Wnt and nuclear factor κB signaling pathways. Moreover, overexpression of AEG-1 partially rescued the inhibitory effects of miR-342-3p mediated on the proliferation, migration, and invasion of osteosarcoma cells. Overall, our results show that miR-342-3p inhibits the proliferation, migration, and invasion of osteosarcoma cells through targeting AEG-1, suggesting a potential target for the development of miRNA-based therapy for osteosarcoma.
format Online
Article
Text
id pubmed-7841055
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Cognizant Communication Corporation
record_format MEDLINE/PubMed
spelling pubmed-78410552021-02-16 MicroRNA-342-3p Inhibits the Proliferation, Migration, and Invasion of Osteosarcoma Cells by Targeting Astrocyte-Elevated Gene-1 (AEG-1) Zhang, Shaokun Liu, Lidi Lv, Zhenshan Li, Qiao Gong, Weiquan Wu, Hong Oncol Res Article Recent studies suggest that microRNAs (miRNAs) are critical regulators in many types of cancer, including osteosarcoma. miR-342-3p has emerged as an important cancer-related miRNA in several types of cancers. However, the functional significance of miR-342-3p in osteosarcoma is unknown. The aims of this study were to investigate whether miR-342-3p is dysregulated in osteosarcoma and to explore the biological function of miR-342-3p in regulating cellular processes of osteosarcoma cells. We found that miR-342-3p expression was significantly decreased in osteosarcoma tissues and cell lines. Overexpression of miR-342-3p inhibits the proliferation, migration, and invasion of osteosarcoma cells. In contrast, the inhibition of miR-342-3p exhibited the opposite effect. Astrocyte-elevated gene-1 (AEG-1) was identified as one of the target genes of miR-342-3p in osteosarcoma cells by bioinformatics analysis, dual-luciferase reporter assay, real-time quantitative polymerase chain reaction, and Western blot analysis. Overexpression of miR-342-3p also inhibited the Wnt and nuclear factor κB signaling pathways. Moreover, overexpression of AEG-1 partially rescued the inhibitory effects of miR-342-3p mediated on the proliferation, migration, and invasion of osteosarcoma cells. Overall, our results show that miR-342-3p inhibits the proliferation, migration, and invasion of osteosarcoma cells through targeting AEG-1, suggesting a potential target for the development of miRNA-based therapy for osteosarcoma. Cognizant Communication Corporation 2017-11-02 /pmc/articles/PMC7841055/ /pubmed/28276315 http://dx.doi.org/10.3727/096504017X14886485417426 Text en Copyright © 2017 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Zhang, Shaokun
Liu, Lidi
Lv, Zhenshan
Li, Qiao
Gong, Weiquan
Wu, Hong
MicroRNA-342-3p Inhibits the Proliferation, Migration, and Invasion of Osteosarcoma Cells by Targeting Astrocyte-Elevated Gene-1 (AEG-1)
title MicroRNA-342-3p Inhibits the Proliferation, Migration, and Invasion of Osteosarcoma Cells by Targeting Astrocyte-Elevated Gene-1 (AEG-1)
title_full MicroRNA-342-3p Inhibits the Proliferation, Migration, and Invasion of Osteosarcoma Cells by Targeting Astrocyte-Elevated Gene-1 (AEG-1)
title_fullStr MicroRNA-342-3p Inhibits the Proliferation, Migration, and Invasion of Osteosarcoma Cells by Targeting Astrocyte-Elevated Gene-1 (AEG-1)
title_full_unstemmed MicroRNA-342-3p Inhibits the Proliferation, Migration, and Invasion of Osteosarcoma Cells by Targeting Astrocyte-Elevated Gene-1 (AEG-1)
title_short MicroRNA-342-3p Inhibits the Proliferation, Migration, and Invasion of Osteosarcoma Cells by Targeting Astrocyte-Elevated Gene-1 (AEG-1)
title_sort microrna-342-3p inhibits the proliferation, migration, and invasion of osteosarcoma cells by targeting astrocyte-elevated gene-1 (aeg-1)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841055/
https://www.ncbi.nlm.nih.gov/pubmed/28276315
http://dx.doi.org/10.3727/096504017X14886485417426
work_keys_str_mv AT zhangshaokun microrna3423pinhibitstheproliferationmigrationandinvasionofosteosarcomacellsbytargetingastrocyteelevatedgene1aeg1
AT liulidi microrna3423pinhibitstheproliferationmigrationandinvasionofosteosarcomacellsbytargetingastrocyteelevatedgene1aeg1
AT lvzhenshan microrna3423pinhibitstheproliferationmigrationandinvasionofosteosarcomacellsbytargetingastrocyteelevatedgene1aeg1
AT liqiao microrna3423pinhibitstheproliferationmigrationandinvasionofosteosarcomacellsbytargetingastrocyteelevatedgene1aeg1
AT gongweiquan microrna3423pinhibitstheproliferationmigrationandinvasionofosteosarcomacellsbytargetingastrocyteelevatedgene1aeg1
AT wuhong microrna3423pinhibitstheproliferationmigrationandinvasionofosteosarcomacellsbytargetingastrocyteelevatedgene1aeg1