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Silencing Transmembrane Protein 45B (TNEM45B) Inhibits Proliferation, Invasion, and Tumorigenesis in Osteosarcoma Cells

Transmembrane protein 45B (TMEM45B) is a member of the TMEM family of proteins and has been reported to be expressed abnormally in different kinds of human tumors. However, the biological function of TMEM45B in osteosarcoma remains unclear. The objective of this study was to investigate the role of...

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Autores principales: Li, Yan, Guo, Wei, Liu, Shen, Zhang, Bin, Yu, Bing-Bing, Yang, Bo, Kan, Shun-Li, Feng, Shi-Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841085/
https://www.ncbi.nlm.nih.gov/pubmed/28244852
http://dx.doi.org/10.3727/096504016X14821477992177
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author Li, Yan
Guo, Wei
Liu, Shen
Zhang, Bin
Yu, Bing-Bing
Yang, Bo
Kan, Shun-Li
Feng, Shi-Qing
author_facet Li, Yan
Guo, Wei
Liu, Shen
Zhang, Bin
Yu, Bing-Bing
Yang, Bo
Kan, Shun-Li
Feng, Shi-Qing
author_sort Li, Yan
collection PubMed
description Transmembrane protein 45B (TMEM45B) is a member of the TMEM family of proteins and has been reported to be expressed abnormally in different kinds of human tumors. However, the biological function of TMEM45B in osteosarcoma remains unclear. The objective of this study was to investigate the role of TMEM45B in regulating the biological behavior of osteosarcoma cells. Our results demonstrated that the expression of TMEM45B at both the protein and mRNA levels was dramatically upregulated in human osteosarcoma cell lines. Knockdown of TMEM45B significantly suppressed the proliferation, migration, and invasion of U2OS cells in vitro. Mechanistically, knockdown of TMEM45B sharply downregulated the expression level of β-catenin, cyclin D1, and c-Myc in U2OS cells. Finally, knockdown of TMEM45B attenuated tumor growth in transplanted U2OS-derived tumors in nude mice. Taken together, our results demonstrated that TMEM45B plays an important role in regulating the proliferation, migration, and invasion of osteosarcoma cells and that its effects on proliferation and invasion were mediated partially through the Wnt/β-catenin signaling pathway. These observations support our belief that TMEM45B may serve as an oncogene in the development and progression of osteosarcoma.
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spelling pubmed-78410852021-02-16 Silencing Transmembrane Protein 45B (TNEM45B) Inhibits Proliferation, Invasion, and Tumorigenesis in Osteosarcoma Cells Li, Yan Guo, Wei Liu, Shen Zhang, Bin Yu, Bing-Bing Yang, Bo Kan, Shun-Li Feng, Shi-Qing Oncol Res Article Transmembrane protein 45B (TMEM45B) is a member of the TMEM family of proteins and has been reported to be expressed abnormally in different kinds of human tumors. However, the biological function of TMEM45B in osteosarcoma remains unclear. The objective of this study was to investigate the role of TMEM45B in regulating the biological behavior of osteosarcoma cells. Our results demonstrated that the expression of TMEM45B at both the protein and mRNA levels was dramatically upregulated in human osteosarcoma cell lines. Knockdown of TMEM45B significantly suppressed the proliferation, migration, and invasion of U2OS cells in vitro. Mechanistically, knockdown of TMEM45B sharply downregulated the expression level of β-catenin, cyclin D1, and c-Myc in U2OS cells. Finally, knockdown of TMEM45B attenuated tumor growth in transplanted U2OS-derived tumors in nude mice. Taken together, our results demonstrated that TMEM45B plays an important role in regulating the proliferation, migration, and invasion of osteosarcoma cells and that its effects on proliferation and invasion were mediated partially through the Wnt/β-catenin signaling pathway. These observations support our belief that TMEM45B may serve as an oncogene in the development and progression of osteosarcoma. Cognizant Communication Corporation 2017-07-05 /pmc/articles/PMC7841085/ /pubmed/28244852 http://dx.doi.org/10.3727/096504016X14821477992177 Text en Copyright © 2017 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Li, Yan
Guo, Wei
Liu, Shen
Zhang, Bin
Yu, Bing-Bing
Yang, Bo
Kan, Shun-Li
Feng, Shi-Qing
Silencing Transmembrane Protein 45B (TNEM45B) Inhibits Proliferation, Invasion, and Tumorigenesis in Osteosarcoma Cells
title Silencing Transmembrane Protein 45B (TNEM45B) Inhibits Proliferation, Invasion, and Tumorigenesis in Osteosarcoma Cells
title_full Silencing Transmembrane Protein 45B (TNEM45B) Inhibits Proliferation, Invasion, and Tumorigenesis in Osteosarcoma Cells
title_fullStr Silencing Transmembrane Protein 45B (TNEM45B) Inhibits Proliferation, Invasion, and Tumorigenesis in Osteosarcoma Cells
title_full_unstemmed Silencing Transmembrane Protein 45B (TNEM45B) Inhibits Proliferation, Invasion, and Tumorigenesis in Osteosarcoma Cells
title_short Silencing Transmembrane Protein 45B (TNEM45B) Inhibits Proliferation, Invasion, and Tumorigenesis in Osteosarcoma Cells
title_sort silencing transmembrane protein 45b (tnem45b) inhibits proliferation, invasion, and tumorigenesis in osteosarcoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841085/
https://www.ncbi.nlm.nih.gov/pubmed/28244852
http://dx.doi.org/10.3727/096504016X14821477992177
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