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MicroRNA-133b Inhibits Cell Proliferation and Invasion in Osteosarcoma by Targeting Sirt1

MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that the miR-133b expression level was decreased while the Sirt1 mRNA expression level was increased in osteosarcoma t...

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Autores principales: Ying, Shi, Jianjun, Huang, Xue, Yi, Shuwei, Yu, Liyuan, Zhang, Jie, Wang, Lixian, Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841126/
https://www.ncbi.nlm.nih.gov/pubmed/28059051
http://dx.doi.org/10.3727/096504016X14826089198805
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author Ying, Shi
Jianjun, Huang
Xue, Yi
Shuwei, Yu
Liyuan, Zhang
Jie, Wang
Lixian, Cheng
author_facet Ying, Shi
Jianjun, Huang
Xue, Yi
Shuwei, Yu
Liyuan, Zhang
Jie, Wang
Lixian, Cheng
author_sort Ying, Shi
collection PubMed
description MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that the miR-133b expression level was decreased while the Sirt1 mRNA expression level was increased in osteosarcoma tissue and cell lines. A low expression of miR-133b was significantly associated with tumor size, distant metastasis, and advanced clinical stage. In addition, osteosarcoma patients with a low miR-133b expression showed a worse prognosis when compared to those with a high level of miR-133b expression. Thus, we identified Sirt1 as a novel direct target of miR-133b. Overexpression of miR-133b suppressed Sirt1 expression and attenuated cell proliferation and invasion. Forced expression of Sirt1 could partly rescue the inhibitory effect of miR-133b in osteosarcoma cells. Our finding also suggested that the inhibitory effects of the miR-133b/Sirt1 axis on osteosarcoma progression were involved in the Wnt/β-catenin pathway. Taken together, these findings will shed light on the role and mechanism of miR-133b in regulating osteosarcoma cell growth via the miR-133b/Sirt1 axis, and miR-133b may serve as a potential therapeutic target in osteosarcoma in the future.
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spelling pubmed-78411262021-02-16 MicroRNA-133b Inhibits Cell Proliferation and Invasion in Osteosarcoma by Targeting Sirt1 Ying, Shi Jianjun, Huang Xue, Yi Shuwei, Yu Liyuan, Zhang Jie, Wang Lixian, Cheng Oncol Res Article MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that the miR-133b expression level was decreased while the Sirt1 mRNA expression level was increased in osteosarcoma tissue and cell lines. A low expression of miR-133b was significantly associated with tumor size, distant metastasis, and advanced clinical stage. In addition, osteosarcoma patients with a low miR-133b expression showed a worse prognosis when compared to those with a high level of miR-133b expression. Thus, we identified Sirt1 as a novel direct target of miR-133b. Overexpression of miR-133b suppressed Sirt1 expression and attenuated cell proliferation and invasion. Forced expression of Sirt1 could partly rescue the inhibitory effect of miR-133b in osteosarcoma cells. Our finding also suggested that the inhibitory effects of the miR-133b/Sirt1 axis on osteosarcoma progression were involved in the Wnt/β-catenin pathway. Taken together, these findings will shed light on the role and mechanism of miR-133b in regulating osteosarcoma cell growth via the miR-133b/Sirt1 axis, and miR-133b may serve as a potential therapeutic target in osteosarcoma in the future. Cognizant Communication Corporation 2017-11-02 /pmc/articles/PMC7841126/ /pubmed/28059051 http://dx.doi.org/10.3727/096504016X14826089198805 Text en Copyright © 2017 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Ying, Shi
Jianjun, Huang
Xue, Yi
Shuwei, Yu
Liyuan, Zhang
Jie, Wang
Lixian, Cheng
MicroRNA-133b Inhibits Cell Proliferation and Invasion in Osteosarcoma by Targeting Sirt1
title MicroRNA-133b Inhibits Cell Proliferation and Invasion in Osteosarcoma by Targeting Sirt1
title_full MicroRNA-133b Inhibits Cell Proliferation and Invasion in Osteosarcoma by Targeting Sirt1
title_fullStr MicroRNA-133b Inhibits Cell Proliferation and Invasion in Osteosarcoma by Targeting Sirt1
title_full_unstemmed MicroRNA-133b Inhibits Cell Proliferation and Invasion in Osteosarcoma by Targeting Sirt1
title_short MicroRNA-133b Inhibits Cell Proliferation and Invasion in Osteosarcoma by Targeting Sirt1
title_sort microrna-133b inhibits cell proliferation and invasion in osteosarcoma by targeting sirt1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841126/
https://www.ncbi.nlm.nih.gov/pubmed/28059051
http://dx.doi.org/10.3727/096504016X14826089198805
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