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MicroRNA-221-3p Plays an Oncogenic Role in Gastric Carcinoma by Inhibiting PTEN Expression

Gastric carcinoma is one of the most common malignancies in men, and microRNA plays a critical role in regulating the signaling networks of gastric carcinoma tumorigenesis and metastasis. We first report the functional characteristics of miR-221-3p in gastric carcinoma. Quantification in gastric car...

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Detalles Bibliográficos
Autores principales: Shi, Jianping, Zhang, Yi, Jin, Nuyun, Li, Yuqin, Wu, Shengtian, Xu, Leiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841127/
https://www.ncbi.nlm.nih.gov/pubmed/27712596
http://dx.doi.org/10.3727/096504016X14756282819385
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author Shi, Jianping
Zhang, Yi
Jin, Nuyun
Li, Yuqin
Wu, Shengtian
Xu, Leiming
author_facet Shi, Jianping
Zhang, Yi
Jin, Nuyun
Li, Yuqin
Wu, Shengtian
Xu, Leiming
author_sort Shi, Jianping
collection PubMed
description Gastric carcinoma is one of the most common malignancies in men, and microRNA plays a critical role in regulating the signaling networks of gastric carcinoma tumorigenesis and metastasis. We first report the functional characteristics of miR-221-3p in gastric carcinoma. Quantification in gastric carcinoma cell lines and tumor samples reveals significantly increasing miR-221-3p expression. Moreover, a high level of miR-221-3p is correlated with a poor prognosis for gastric carcinoma patients. Ectopic miR-221-3p expression significantly promotes gastric carcinoma cell proliferation, invasion, and sphere formation, while silencing miR-221-3p significantly inhibits these abilities in gastric carcinoma cells. Tests in vivo showed that miR-221-3p significantly promotes tumor growth in xenograft mouse models. In this study, we reveal that miR-221-3p targets PTEN mRNA and downregulates PTEN, which is the possible mechanism of miR-221-3p-induced oncogenic properties. Collectively, we reveal a critical role for miR-221-3p in gastric carcinoma development and progression.
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spelling pubmed-78411272021-02-16 MicroRNA-221-3p Plays an Oncogenic Role in Gastric Carcinoma by Inhibiting PTEN Expression Shi, Jianping Zhang, Yi Jin, Nuyun Li, Yuqin Wu, Shengtian Xu, Leiming Oncol Res Article Gastric carcinoma is one of the most common malignancies in men, and microRNA plays a critical role in regulating the signaling networks of gastric carcinoma tumorigenesis and metastasis. We first report the functional characteristics of miR-221-3p in gastric carcinoma. Quantification in gastric carcinoma cell lines and tumor samples reveals significantly increasing miR-221-3p expression. Moreover, a high level of miR-221-3p is correlated with a poor prognosis for gastric carcinoma patients. Ectopic miR-221-3p expression significantly promotes gastric carcinoma cell proliferation, invasion, and sphere formation, while silencing miR-221-3p significantly inhibits these abilities in gastric carcinoma cells. Tests in vivo showed that miR-221-3p significantly promotes tumor growth in xenograft mouse models. In this study, we reveal that miR-221-3p targets PTEN mRNA and downregulates PTEN, which is the possible mechanism of miR-221-3p-induced oncogenic properties. Collectively, we reveal a critical role for miR-221-3p in gastric carcinoma development and progression. Cognizant Communication Corporation 2017-04-14 /pmc/articles/PMC7841127/ /pubmed/27712596 http://dx.doi.org/10.3727/096504016X14756282819385 Text en Copyright © 2017 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Shi, Jianping
Zhang, Yi
Jin, Nuyun
Li, Yuqin
Wu, Shengtian
Xu, Leiming
MicroRNA-221-3p Plays an Oncogenic Role in Gastric Carcinoma by Inhibiting PTEN Expression
title MicroRNA-221-3p Plays an Oncogenic Role in Gastric Carcinoma by Inhibiting PTEN Expression
title_full MicroRNA-221-3p Plays an Oncogenic Role in Gastric Carcinoma by Inhibiting PTEN Expression
title_fullStr MicroRNA-221-3p Plays an Oncogenic Role in Gastric Carcinoma by Inhibiting PTEN Expression
title_full_unstemmed MicroRNA-221-3p Plays an Oncogenic Role in Gastric Carcinoma by Inhibiting PTEN Expression
title_short MicroRNA-221-3p Plays an Oncogenic Role in Gastric Carcinoma by Inhibiting PTEN Expression
title_sort microrna-221-3p plays an oncogenic role in gastric carcinoma by inhibiting pten expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841127/
https://www.ncbi.nlm.nih.gov/pubmed/27712596
http://dx.doi.org/10.3727/096504016X14756282819385
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