Knockdown of Serine–Arginine Protein Kinase 1 Inhibits the Growth and Migration in Renal Cell Carcinoma Cells

The pre-mRNA splicing regulator serine–arginine protein kinase 1 (SRPK1), a member of the SR kinase family, plays an essential role in cancer development and various pathophysiological processes. However, its expression pattern and functions in renal cell carcinoma (RCC) remain unknown. Therefore, t...

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Autores principales: Han, Xingtao, Yang, Jinjian, Jia, Zhankui, Wei, Pengtao, Zhang, Han, Lv, Wenwei, Sun, Jiantao, Huo, Qingxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841233/
https://www.ncbi.nlm.nih.gov/pubmed/27662590
http://dx.doi.org/10.3727/096504016X14743324568129
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author Han, Xingtao
Yang, Jinjian
Jia, Zhankui
Wei, Pengtao
Zhang, Han
Lv, Wenwei
Sun, Jiantao
Huo, Qingxiang
author_facet Han, Xingtao
Yang, Jinjian
Jia, Zhankui
Wei, Pengtao
Zhang, Han
Lv, Wenwei
Sun, Jiantao
Huo, Qingxiang
author_sort Han, Xingtao
collection PubMed
description The pre-mRNA splicing regulator serine–arginine protein kinase 1 (SRPK1), a member of the SR kinase family, plays an essential role in cancer development and various pathophysiological processes. However, its expression pattern and functions in renal cell carcinoma (RCC) remain unknown. Therefore, the aim of this study was to assess the role of SRPK1 in RCC. Our data showed that SRPK1 was significantly upregulated in human RCC tissues and cell lines. SRPK1 interference significantly inhibited the proliferation of RCC cells and inhibited tumor growth in vivo. In addition, SRPK1 interference also suppressed migration and invasion in RCC cells. A mechanistic study showed that SRPK1 interference inhibited the phosphorylation of PI3K and Akt in RCC cells. In conclusion, our findings suggest that SRPK1 interference inhibits the growth and invasion of RCC cells through suppressing the PI3K/Akt signaling pathway. Thus, SRPK1 might be a therapeutic target for the treatment of RCC.
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spelling pubmed-78412332021-02-16 Knockdown of Serine–Arginine Protein Kinase 1 Inhibits the Growth and Migration in Renal Cell Carcinoma Cells Han, Xingtao Yang, Jinjian Jia, Zhankui Wei, Pengtao Zhang, Han Lv, Wenwei Sun, Jiantao Huo, Qingxiang Oncol Res Article The pre-mRNA splicing regulator serine–arginine protein kinase 1 (SRPK1), a member of the SR kinase family, plays an essential role in cancer development and various pathophysiological processes. However, its expression pattern and functions in renal cell carcinoma (RCC) remain unknown. Therefore, the aim of this study was to assess the role of SRPK1 in RCC. Our data showed that SRPK1 was significantly upregulated in human RCC tissues and cell lines. SRPK1 interference significantly inhibited the proliferation of RCC cells and inhibited tumor growth in vivo. In addition, SRPK1 interference also suppressed migration and invasion in RCC cells. A mechanistic study showed that SRPK1 interference inhibited the phosphorylation of PI3K and Akt in RCC cells. In conclusion, our findings suggest that SRPK1 interference inhibits the growth and invasion of RCC cells through suppressing the PI3K/Akt signaling pathway. Thus, SRPK1 might be a therapeutic target for the treatment of RCC. Cognizant Communication Corporation 2017-03-13 /pmc/articles/PMC7841233/ /pubmed/27662590 http://dx.doi.org/10.3727/096504016X14743324568129 Text en Copyright © 2017 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Han, Xingtao
Yang, Jinjian
Jia, Zhankui
Wei, Pengtao
Zhang, Han
Lv, Wenwei
Sun, Jiantao
Huo, Qingxiang
Knockdown of Serine–Arginine Protein Kinase 1 Inhibits the Growth and Migration in Renal Cell Carcinoma Cells
title Knockdown of Serine–Arginine Protein Kinase 1 Inhibits the Growth and Migration in Renal Cell Carcinoma Cells
title_full Knockdown of Serine–Arginine Protein Kinase 1 Inhibits the Growth and Migration in Renal Cell Carcinoma Cells
title_fullStr Knockdown of Serine–Arginine Protein Kinase 1 Inhibits the Growth and Migration in Renal Cell Carcinoma Cells
title_full_unstemmed Knockdown of Serine–Arginine Protein Kinase 1 Inhibits the Growth and Migration in Renal Cell Carcinoma Cells
title_short Knockdown of Serine–Arginine Protein Kinase 1 Inhibits the Growth and Migration in Renal Cell Carcinoma Cells
title_sort knockdown of serine–arginine protein kinase 1 inhibits the growth and migration in renal cell carcinoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841233/
https://www.ncbi.nlm.nih.gov/pubmed/27662590
http://dx.doi.org/10.3727/096504016X14743324568129
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