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miR-144-5p and miR-451a Inhibit the Growth of Cholangiocarcinoma Cells Through Decreasing the Expression of ST8SIA4

Accumulating evidences indicate that non-coding RNAs play crucial roles in the progression of an extensive range of carcinomas. This study aimed to investigate the action mechanism of miR-144-5p and miR-451a in cholangiocarcinoma. We found that miR-144-5p and miR-451a were significantly decreased in...

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Autores principales: Fu, Wan, Yu, Guangcai, Liang, Junnan, Fan, Pan, Dong, Keshuai, Zhang, Bixiang, Chen, Xiaoping, Zhu, Hong, Chu, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841262/
https://www.ncbi.nlm.nih.gov/pubmed/33520692
http://dx.doi.org/10.3389/fonc.2020.563486
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author Fu, Wan
Yu, Guangcai
Liang, Junnan
Fan, Pan
Dong, Keshuai
Zhang, Bixiang
Chen, Xiaoping
Zhu, Hong
Chu, Liang
author_facet Fu, Wan
Yu, Guangcai
Liang, Junnan
Fan, Pan
Dong, Keshuai
Zhang, Bixiang
Chen, Xiaoping
Zhu, Hong
Chu, Liang
author_sort Fu, Wan
collection PubMed
description Accumulating evidences indicate that non-coding RNAs play crucial roles in the progression of an extensive range of carcinomas. This study aimed to investigate the action mechanism of miR-144-5p and miR-451a in cholangiocarcinoma. We found that miR-144-5p and miR-451a were significantly decreased in cholangiocarcinoma patient samples compared to the adjacent normal bile duct samples. The downregulation of these two miRNAs was correlated with a more advanced disease state of cholangiocarcinoma patients. Overexpression of miR-144-5p and miR-451a suppressed the proliferation, invasion and migration of cholangiocarcinoma cells in vitro and inhibited xenograft tumor growth. Knockdown of these two miRNAs had the opposite effects. miR-144-5p and miR-451a regulated the expression of ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (ST8SIA4), and presented a correlation with ST8SIA4 in patient samples. Overexpression of ST8SIA4 promoted the proliferation, invasion and migration of cholangiocarcinoma cells, and the changes were reversed by upregulating the expression of miR-144-5p and miR-451a. Our findings indicated that miR-144-5p and miR-451a displayed a tumor suppressor role through decreasing the expression of ST8SIA4 in cholangiocarcinoma.
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spelling pubmed-78412622021-01-29 miR-144-5p and miR-451a Inhibit the Growth of Cholangiocarcinoma Cells Through Decreasing the Expression of ST8SIA4 Fu, Wan Yu, Guangcai Liang, Junnan Fan, Pan Dong, Keshuai Zhang, Bixiang Chen, Xiaoping Zhu, Hong Chu, Liang Front Oncol Oncology Accumulating evidences indicate that non-coding RNAs play crucial roles in the progression of an extensive range of carcinomas. This study aimed to investigate the action mechanism of miR-144-5p and miR-451a in cholangiocarcinoma. We found that miR-144-5p and miR-451a were significantly decreased in cholangiocarcinoma patient samples compared to the adjacent normal bile duct samples. The downregulation of these two miRNAs was correlated with a more advanced disease state of cholangiocarcinoma patients. Overexpression of miR-144-5p and miR-451a suppressed the proliferation, invasion and migration of cholangiocarcinoma cells in vitro and inhibited xenograft tumor growth. Knockdown of these two miRNAs had the opposite effects. miR-144-5p and miR-451a regulated the expression of ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (ST8SIA4), and presented a correlation with ST8SIA4 in patient samples. Overexpression of ST8SIA4 promoted the proliferation, invasion and migration of cholangiocarcinoma cells, and the changes were reversed by upregulating the expression of miR-144-5p and miR-451a. Our findings indicated that miR-144-5p and miR-451a displayed a tumor suppressor role through decreasing the expression of ST8SIA4 in cholangiocarcinoma. Frontiers Media S.A. 2021-01-14 /pmc/articles/PMC7841262/ /pubmed/33520692 http://dx.doi.org/10.3389/fonc.2020.563486 Text en Copyright © 2021 Fu, Yu, Liang, Fan, Dong, Zhang, Chen, Zhu and Chu http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Fu, Wan
Yu, Guangcai
Liang, Junnan
Fan, Pan
Dong, Keshuai
Zhang, Bixiang
Chen, Xiaoping
Zhu, Hong
Chu, Liang
miR-144-5p and miR-451a Inhibit the Growth of Cholangiocarcinoma Cells Through Decreasing the Expression of ST8SIA4
title miR-144-5p and miR-451a Inhibit the Growth of Cholangiocarcinoma Cells Through Decreasing the Expression of ST8SIA4
title_full miR-144-5p and miR-451a Inhibit the Growth of Cholangiocarcinoma Cells Through Decreasing the Expression of ST8SIA4
title_fullStr miR-144-5p and miR-451a Inhibit the Growth of Cholangiocarcinoma Cells Through Decreasing the Expression of ST8SIA4
title_full_unstemmed miR-144-5p and miR-451a Inhibit the Growth of Cholangiocarcinoma Cells Through Decreasing the Expression of ST8SIA4
title_short miR-144-5p and miR-451a Inhibit the Growth of Cholangiocarcinoma Cells Through Decreasing the Expression of ST8SIA4
title_sort mir-144-5p and mir-451a inhibit the growth of cholangiocarcinoma cells through decreasing the expression of st8sia4
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841262/
https://www.ncbi.nlm.nih.gov/pubmed/33520692
http://dx.doi.org/10.3389/fonc.2020.563486
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