Synthesis and characterization of hesperetin derivatives and toxicity level of the zebrafish model

Hesperetin derivatives were synthesized through the esterification of acid chlorides with hesperetin under ambient reaction conditions with good yields. The product was confirmed using different spectral techniques. It was treated on zebrafish embryos to study the lethality, phenotypic deformities, and toxicity level of the compound. In that assessment, embryos showed lethality towards 3e at the minimal concentration. It assesses slow heartbeat since the compound loaded, the curvature on the back, upcurved fish, Cardiac chamber bulging, and poor survival rate in 72 h. 3a shows less toxicity more than other compounds. It shows only pericardial edema at higher concentration and 3c induced pericardial edema and upcurved tail at a medium range of the concentration. But both compounds were shown a good survival ratio at the minimal concentration.