Expression, purification, and characterization of a membrane-associated cyclic oligo-adenylate degrader from Sulfolobus islandicus

Type III CRISPR-cas systems initiate cyclic oligo-adenylate (cOA) signaling to initiate immune response. Previously, we identified that a membrane-associated DHH-DHHA1 family protein from Sulfolobus islandicus efficiently degrades cOA. Here, we provide detailed protocols for expression and purificat...

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Detalles Bibliográficos
Autores principales: Zhao, Ruiliang, Yang, Yang, Yang, Ke, Han, Wenyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841402/
https://www.ncbi.nlm.nih.gov/pubmed/33537681
http://dx.doi.org/10.1016/j.xpro.2021.100299

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