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Lipase Immobilization on Macroporous ZIF-8 for Enhanced Enzymatic Biodiesel Production

[Image: see text] Immobilization of enzyme on metal–organic frameworks (MOFs) has drawn increasing interest owing to their many well-recognized characteristics. However, the pore sizes of MOFs (mostly micropores and mesopores) limit their application for enzyme immobilization to a great extent owing...

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Autores principales: Hu, Yingli, Zhou, Hao, Dai, Lingmei, Liu, Dehua, Al-Zuhair, Sulaiman, Du, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841922/
https://www.ncbi.nlm.nih.gov/pubmed/33521453
http://dx.doi.org/10.1021/acsomega.0c05225
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author Hu, Yingli
Zhou, Hao
Dai, Lingmei
Liu, Dehua
Al-Zuhair, Sulaiman
Du, Wei
author_facet Hu, Yingli
Zhou, Hao
Dai, Lingmei
Liu, Dehua
Al-Zuhair, Sulaiman
Du, Wei
author_sort Hu, Yingli
collection PubMed
description [Image: see text] Immobilization of enzyme on metal–organic frameworks (MOFs) has drawn increasing interest owing to their many well-recognized characteristics. However, the pore sizes of MOFs (mostly micropores and mesopores) limit their application for enzyme immobilization to a great extent owing to the large size of enzyme molecules. Synthesis of MOFs with macropores would therefore solve this problem, typically encountered with conventional MOFs. In this work, macroporous zeolitic imidazolate frameworks (ZIF-8), referred to as M-ZIF-8, were synthesized and used for immobilization of Aspergillus niger lipase (ANL). Immobilization efficiency using M-ZIF-8 and enzymatic catalytic performance for biodiesel preparation were investigated. The immobilized ANL on M-ZIF-8 (ANL@M-ZIF-8) showed higher enzymatic activity (6.5-fold), activity recovery (3.8-fold), thermal stability (1.4- and 3.4-fold at 80 and 100 °C, respectively), reusability (after five cycles, 68% of initial activity was maintained), and porosity than ANL on conventional ZIF-8 (ANL/ZIF-8). In addition, by using ANL@M-ZIF-8 for catalyzing a biodiesel production reaction, a higher fatty acid methyl ester yield was achieved.
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spelling pubmed-78419222021-01-29 Lipase Immobilization on Macroporous ZIF-8 for Enhanced Enzymatic Biodiesel Production Hu, Yingli Zhou, Hao Dai, Lingmei Liu, Dehua Al-Zuhair, Sulaiman Du, Wei ACS Omega [Image: see text] Immobilization of enzyme on metal–organic frameworks (MOFs) has drawn increasing interest owing to their many well-recognized characteristics. However, the pore sizes of MOFs (mostly micropores and mesopores) limit their application for enzyme immobilization to a great extent owing to the large size of enzyme molecules. Synthesis of MOFs with macropores would therefore solve this problem, typically encountered with conventional MOFs. In this work, macroporous zeolitic imidazolate frameworks (ZIF-8), referred to as M-ZIF-8, were synthesized and used for immobilization of Aspergillus niger lipase (ANL). Immobilization efficiency using M-ZIF-8 and enzymatic catalytic performance for biodiesel preparation were investigated. The immobilized ANL on M-ZIF-8 (ANL@M-ZIF-8) showed higher enzymatic activity (6.5-fold), activity recovery (3.8-fold), thermal stability (1.4- and 3.4-fold at 80 and 100 °C, respectively), reusability (after five cycles, 68% of initial activity was maintained), and porosity than ANL on conventional ZIF-8 (ANL/ZIF-8). In addition, by using ANL@M-ZIF-8 for catalyzing a biodiesel production reaction, a higher fatty acid methyl ester yield was achieved. American Chemical Society 2021-01-14 /pmc/articles/PMC7841922/ /pubmed/33521453 http://dx.doi.org/10.1021/acsomega.0c05225 Text en © 2021 The Authors. Published by American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Hu, Yingli
Zhou, Hao
Dai, Lingmei
Liu, Dehua
Al-Zuhair, Sulaiman
Du, Wei
Lipase Immobilization on Macroporous ZIF-8 for Enhanced Enzymatic Biodiesel Production
title Lipase Immobilization on Macroporous ZIF-8 for Enhanced Enzymatic Biodiesel Production
title_full Lipase Immobilization on Macroporous ZIF-8 for Enhanced Enzymatic Biodiesel Production
title_fullStr Lipase Immobilization on Macroporous ZIF-8 for Enhanced Enzymatic Biodiesel Production
title_full_unstemmed Lipase Immobilization on Macroporous ZIF-8 for Enhanced Enzymatic Biodiesel Production
title_short Lipase Immobilization on Macroporous ZIF-8 for Enhanced Enzymatic Biodiesel Production
title_sort lipase immobilization on macroporous zif-8 for enhanced enzymatic biodiesel production
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841922/
https://www.ncbi.nlm.nih.gov/pubmed/33521453
http://dx.doi.org/10.1021/acsomega.0c05225
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