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Assessment of the Antileishmanial Potential of Cassia fistula Leaf Extract
[Image: see text] Cassia fistula has a wide array of biologically active and therapeutically important class of compounds. Leishmania donovani important drug targets, sterol 24-c methyltransferase (LdSMT), trypanothione reductase (LdTR), pteridine reductase (LdPTR1), and nucleoside hydrolase (LdNH),...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841934/ https://www.ncbi.nlm.nih.gov/pubmed/33521470 http://dx.doi.org/10.1021/acsomega.0c05629 |
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author | Tabrez, Shams Rahman, Fazlur Ali, Rahat Alouffi, Abdulaziz S. Alshehri, Bader Mohammed Alshammari, Fahdah Ayed Alaidarous, Mohammed A. Banawas, Saeed Bin Dukhyil, Abdul Aziz Rub, Abdur |
author_facet | Tabrez, Shams Rahman, Fazlur Ali, Rahat Alouffi, Abdulaziz S. Alshehri, Bader Mohammed Alshammari, Fahdah Ayed Alaidarous, Mohammed A. Banawas, Saeed Bin Dukhyil, Abdul Aziz Rub, Abdur |
author_sort | Tabrez, Shams |
collection | PubMed |
description | [Image: see text] Cassia fistula has a wide array of biologically active and therapeutically important class of compounds. Leishmania donovani important drug targets, sterol 24-c methyltransferase (LdSMT), trypanothione reductase (LdTR), pteridine reductase (LdPTR1), and nucleoside hydrolase (LdNH), were modelled, and molecular docking was performed against the abundant phytochemicals of its leaf extract. Molecular docking results provided the significant prima facie evidence of the leaf extract to have antileishmanial potential. To confirm this, we performed in vitro antileishmanial and cytotoxicity assays. Methanolic extract of C. fistula leaves showed growth inhibition and proliferation of L. donovani promastigote with an IC(50) value of 43.31 ± 4.202 μg/mL. It also inhibited the growth of intra-macrophagic amastigotes with an IC(50) value of 80.76 ± 3.626 μg/mL. C. fistula extract was found cytotoxic at a very high concentration on human macrophages (CC(50) = 626 ± 39 μg/mL). Annexin V/propidium iodide (PI) staining assay suggested partial apoptosis induction in parasites by C. fistula to exert its antileishmanial activity. Here, for the first time, we have shown the antileishmanial potential of C. fistula leaves. Overall, our results could open new insight for an affordable and natural antileishmanial with high efficacy and less toxicity. |
format | Online Article Text |
id | pubmed-7841934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-78419342021-01-29 Assessment of the Antileishmanial Potential of Cassia fistula Leaf Extract Tabrez, Shams Rahman, Fazlur Ali, Rahat Alouffi, Abdulaziz S. Alshehri, Bader Mohammed Alshammari, Fahdah Ayed Alaidarous, Mohammed A. Banawas, Saeed Bin Dukhyil, Abdul Aziz Rub, Abdur ACS Omega [Image: see text] Cassia fistula has a wide array of biologically active and therapeutically important class of compounds. Leishmania donovani important drug targets, sterol 24-c methyltransferase (LdSMT), trypanothione reductase (LdTR), pteridine reductase (LdPTR1), and nucleoside hydrolase (LdNH), were modelled, and molecular docking was performed against the abundant phytochemicals of its leaf extract. Molecular docking results provided the significant prima facie evidence of the leaf extract to have antileishmanial potential. To confirm this, we performed in vitro antileishmanial and cytotoxicity assays. Methanolic extract of C. fistula leaves showed growth inhibition and proliferation of L. donovani promastigote with an IC(50) value of 43.31 ± 4.202 μg/mL. It also inhibited the growth of intra-macrophagic amastigotes with an IC(50) value of 80.76 ± 3.626 μg/mL. C. fistula extract was found cytotoxic at a very high concentration on human macrophages (CC(50) = 626 ± 39 μg/mL). Annexin V/propidium iodide (PI) staining assay suggested partial apoptosis induction in parasites by C. fistula to exert its antileishmanial activity. Here, for the first time, we have shown the antileishmanial potential of C. fistula leaves. Overall, our results could open new insight for an affordable and natural antileishmanial with high efficacy and less toxicity. American Chemical Society 2021-01-11 /pmc/articles/PMC7841934/ /pubmed/33521470 http://dx.doi.org/10.1021/acsomega.0c05629 Text en © 2021 The Authors. Published by American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
spellingShingle | Tabrez, Shams Rahman, Fazlur Ali, Rahat Alouffi, Abdulaziz S. Alshehri, Bader Mohammed Alshammari, Fahdah Ayed Alaidarous, Mohammed A. Banawas, Saeed Bin Dukhyil, Abdul Aziz Rub, Abdur Assessment of the Antileishmanial Potential of Cassia fistula Leaf Extract |
title | Assessment of the Antileishmanial Potential of Cassia
fistula Leaf Extract |
title_full | Assessment of the Antileishmanial Potential of Cassia
fistula Leaf Extract |
title_fullStr | Assessment of the Antileishmanial Potential of Cassia
fistula Leaf Extract |
title_full_unstemmed | Assessment of the Antileishmanial Potential of Cassia
fistula Leaf Extract |
title_short | Assessment of the Antileishmanial Potential of Cassia
fistula Leaf Extract |
title_sort | assessment of the antileishmanial potential of cassia
fistula leaf extract |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841934/ https://www.ncbi.nlm.nih.gov/pubmed/33521470 http://dx.doi.org/10.1021/acsomega.0c05629 |
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