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Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells
Breast cancer is the most commonly occurring cancer among women. MicroRNAs as noncoding small RNA molecules play pivotal roles in cancer-related biological processes. Increased levels of microRNA-29a in the serum of breast cancer patients have been reported. Since heat shock proteins (HSPs) play imp...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Cognizant Communication Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842558/ https://www.ncbi.nlm.nih.gov/pubmed/26802653 http://dx.doi.org/10.3727/096504015X14478843952906 |
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author | Choghaei, Encieh Khamisipour, Gholamreza Falahati, Mojtaba Naeimi, Behrooz Mossahebi-Mohammadi, Majid Tahmasebi, Rahim Hasanpour, Mojtaba Shamsian, Shakib Hashemi, Zahra Sadat |
author_facet | Choghaei, Encieh Khamisipour, Gholamreza Falahati, Mojtaba Naeimi, Behrooz Mossahebi-Mohammadi, Majid Tahmasebi, Rahim Hasanpour, Mojtaba Shamsian, Shakib Hashemi, Zahra Sadat |
author_sort | Choghaei, Encieh |
collection | PubMed |
description | Breast cancer is the most commonly occurring cancer among women. MicroRNAs as noncoding small RNA molecules play pivotal roles in cancer-related biological processes. Increased levels of microRNA-29a in the serum of breast cancer patients have been reported. Since heat shock proteins (HSPs) play important roles in cell events, the quantitative fluctuations in their cellular levels could be deemed as key indicators of how the exerted treatment alters cell behavior. In this regard, using an antisense small RNA, we attempted to investigate the effects of miR-29a knockdown on the expression of HSPs genes in the MCF-7 breast cancer cell line. MCF-7 cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium with 10% FBS. Studied cells were subdivided into five groups: treated with scramble, anti-miR-29a, anti-miR-29a + Taxol, Taxol, and control. Taxol was added 24 h post-anti-miR transfection and RNA extraction, and cDNA synthesis was done 48 h later. The changes in expression of HSP27, HSP40, HSP60, HSP70, and HSP90 were evaluated by real-time PCR. Our results revealed that inhibitors of microRNA-29a promote apoptosis through upregulation of HSP60 level and downregulation of HSP27, HSP40, HSP70, and HSP90 levels and could be contemplated as a compelling alternative for Taxol employment with similar effects and/or to sensitize cancer cells to chemotherapy with fewer side effects. |
format | Online Article Text |
id | pubmed-7842558 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Cognizant Communication Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-78425582021-02-16 Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells Choghaei, Encieh Khamisipour, Gholamreza Falahati, Mojtaba Naeimi, Behrooz Mossahebi-Mohammadi, Majid Tahmasebi, Rahim Hasanpour, Mojtaba Shamsian, Shakib Hashemi, Zahra Sadat Oncol Res Article Breast cancer is the most commonly occurring cancer among women. MicroRNAs as noncoding small RNA molecules play pivotal roles in cancer-related biological processes. Increased levels of microRNA-29a in the serum of breast cancer patients have been reported. Since heat shock proteins (HSPs) play important roles in cell events, the quantitative fluctuations in their cellular levels could be deemed as key indicators of how the exerted treatment alters cell behavior. In this regard, using an antisense small RNA, we attempted to investigate the effects of miR-29a knockdown on the expression of HSPs genes in the MCF-7 breast cancer cell line. MCF-7 cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium with 10% FBS. Studied cells were subdivided into five groups: treated with scramble, anti-miR-29a, anti-miR-29a + Taxol, Taxol, and control. Taxol was added 24 h post-anti-miR transfection and RNA extraction, and cDNA synthesis was done 48 h later. The changes in expression of HSP27, HSP40, HSP60, HSP70, and HSP90 were evaluated by real-time PCR. Our results revealed that inhibitors of microRNA-29a promote apoptosis through upregulation of HSP60 level and downregulation of HSP27, HSP40, HSP70, and HSP90 levels and could be contemplated as a compelling alternative for Taxol employment with similar effects and/or to sensitize cancer cells to chemotherapy with fewer side effects. Cognizant Communication Corporation 2016-01-21 /pmc/articles/PMC7842558/ /pubmed/26802653 http://dx.doi.org/10.3727/096504015X14478843952906 Text en Copyright © 2016 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License. |
spellingShingle | Article Choghaei, Encieh Khamisipour, Gholamreza Falahati, Mojtaba Naeimi, Behrooz Mossahebi-Mohammadi, Majid Tahmasebi, Rahim Hasanpour, Mojtaba Shamsian, Shakib Hashemi, Zahra Sadat Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells |
title | Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells |
title_full | Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells |
title_fullStr | Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells |
title_full_unstemmed | Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells |
title_short | Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells |
title_sort | knockdown of microrna-29a changes the expression of heat shock proteins in breast carcinoma mcf-7 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842558/ https://www.ncbi.nlm.nih.gov/pubmed/26802653 http://dx.doi.org/10.3727/096504015X14478843952906 |
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