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Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells

Breast cancer is the most commonly occurring cancer among women. MicroRNAs as noncoding small RNA molecules play pivotal roles in cancer-related biological processes. Increased levels of microRNA-29a in the serum of breast cancer patients have been reported. Since heat shock proteins (HSPs) play imp...

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Autores principales: Choghaei, Encieh, Khamisipour, Gholamreza, Falahati, Mojtaba, Naeimi, Behrooz, Mossahebi-Mohammadi, Majid, Tahmasebi, Rahim, Hasanpour, Mojtaba, Shamsian, Shakib, Hashemi, Zahra Sadat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842558/
https://www.ncbi.nlm.nih.gov/pubmed/26802653
http://dx.doi.org/10.3727/096504015X14478843952906
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author Choghaei, Encieh
Khamisipour, Gholamreza
Falahati, Mojtaba
Naeimi, Behrooz
Mossahebi-Mohammadi, Majid
Tahmasebi, Rahim
Hasanpour, Mojtaba
Shamsian, Shakib
Hashemi, Zahra Sadat
author_facet Choghaei, Encieh
Khamisipour, Gholamreza
Falahati, Mojtaba
Naeimi, Behrooz
Mossahebi-Mohammadi, Majid
Tahmasebi, Rahim
Hasanpour, Mojtaba
Shamsian, Shakib
Hashemi, Zahra Sadat
author_sort Choghaei, Encieh
collection PubMed
description Breast cancer is the most commonly occurring cancer among women. MicroRNAs as noncoding small RNA molecules play pivotal roles in cancer-related biological processes. Increased levels of microRNA-29a in the serum of breast cancer patients have been reported. Since heat shock proteins (HSPs) play important roles in cell events, the quantitative fluctuations in their cellular levels could be deemed as key indicators of how the exerted treatment alters cell behavior. In this regard, using an antisense small RNA, we attempted to investigate the effects of miR-29a knockdown on the expression of HSPs genes in the MCF-7 breast cancer cell line. MCF-7 cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium with 10% FBS. Studied cells were subdivided into five groups: treated with scramble, anti-miR-29a, anti-miR-29a + Taxol, Taxol, and control. Taxol was added 24 h post-anti-miR transfection and RNA extraction, and cDNA synthesis was done 48 h later. The changes in expression of HSP27, HSP40, HSP60, HSP70, and HSP90 were evaluated by real-time PCR. Our results revealed that inhibitors of microRNA-29a promote apoptosis through upregulation of HSP60 level and downregulation of HSP27, HSP40, HSP70, and HSP90 levels and could be contemplated as a compelling alternative for Taxol employment with similar effects and/or to sensitize cancer cells to chemotherapy with fewer side effects.
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spelling pubmed-78425582021-02-16 Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells Choghaei, Encieh Khamisipour, Gholamreza Falahati, Mojtaba Naeimi, Behrooz Mossahebi-Mohammadi, Majid Tahmasebi, Rahim Hasanpour, Mojtaba Shamsian, Shakib Hashemi, Zahra Sadat Oncol Res Article Breast cancer is the most commonly occurring cancer among women. MicroRNAs as noncoding small RNA molecules play pivotal roles in cancer-related biological processes. Increased levels of microRNA-29a in the serum of breast cancer patients have been reported. Since heat shock proteins (HSPs) play important roles in cell events, the quantitative fluctuations in their cellular levels could be deemed as key indicators of how the exerted treatment alters cell behavior. In this regard, using an antisense small RNA, we attempted to investigate the effects of miR-29a knockdown on the expression of HSPs genes in the MCF-7 breast cancer cell line. MCF-7 cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium with 10% FBS. Studied cells were subdivided into five groups: treated with scramble, anti-miR-29a, anti-miR-29a + Taxol, Taxol, and control. Taxol was added 24 h post-anti-miR transfection and RNA extraction, and cDNA synthesis was done 48 h later. The changes in expression of HSP27, HSP40, HSP60, HSP70, and HSP90 were evaluated by real-time PCR. Our results revealed that inhibitors of microRNA-29a promote apoptosis through upregulation of HSP60 level and downregulation of HSP27, HSP40, HSP70, and HSP90 levels and could be contemplated as a compelling alternative for Taxol employment with similar effects and/or to sensitize cancer cells to chemotherapy with fewer side effects. Cognizant Communication Corporation 2016-01-21 /pmc/articles/PMC7842558/ /pubmed/26802653 http://dx.doi.org/10.3727/096504015X14478843952906 Text en Copyright © 2016 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Choghaei, Encieh
Khamisipour, Gholamreza
Falahati, Mojtaba
Naeimi, Behrooz
Mossahebi-Mohammadi, Majid
Tahmasebi, Rahim
Hasanpour, Mojtaba
Shamsian, Shakib
Hashemi, Zahra Sadat
Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells
title Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells
title_full Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells
title_fullStr Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells
title_full_unstemmed Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells
title_short Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells
title_sort knockdown of microrna-29a changes the expression of heat shock proteins in breast carcinoma mcf-7 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842558/
https://www.ncbi.nlm.nih.gov/pubmed/26802653
http://dx.doi.org/10.3727/096504015X14478843952906
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