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LACTB mRNA expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis

This study aimed to find the prognostic value of Beta-lactamase-like (LACTB) in pancreatic adenocarcinoma (PAAD) patients. The mRNA expression of LACTB was upregulated in PAAD and was correlated with vital status (P = 0.0199). The immunoreactive scores of LACTB protein in human PAAD tissues were sig...

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Autores principales: Xie, Jian, Peng, Yang, Chen, Xiaoyu, Li, Qigang, Jian, Bin, Wen, Zelin, Liu, Shengchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842907/
https://www.ncbi.nlm.nih.gov/pubmed/33507917
http://dx.doi.org/10.1371/journal.pone.0245908
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author Xie, Jian
Peng, Yang
Chen, Xiaoyu
Li, Qigang
Jian, Bin
Wen, Zelin
Liu, Shengchun
author_facet Xie, Jian
Peng, Yang
Chen, Xiaoyu
Li, Qigang
Jian, Bin
Wen, Zelin
Liu, Shengchun
author_sort Xie, Jian
collection PubMed
description This study aimed to find the prognostic value of Beta-lactamase-like (LACTB) in pancreatic adenocarcinoma (PAAD) patients. The mRNA expression of LACTB was upregulated in PAAD and was correlated with vital status (P = 0.0199). The immunoreactive scores of LACTB protein in human PAAD tissues were significantly higher than those in adjacent noncancerous pancreatic tissues. Receiver operating characteristic (ROC) curve assessment showed that LACTB mRNA expression has high diagnostic value in PAAD. Kaplan-Meier curve and Cox analyses suggested that patients with high LACTB mRNA expression have a poor prognosis, indicating that LACTB mRNA is an independent prognostic factor for overall survival [hazard ratio (HR) = 1.72, P = 0.015, 95% confidence interval (CI) = 1.106–2.253] and disease-specific survival (HR = 1.97, P = 0.004, 95% CI = 1.238–3.152) of PAAD patients. Gene set enrichment analysis (GSEA) revealed that hallmark_g2m_checkpoint, hallmark_myc_targets_v1, hallmark_e2f_targets, and kegg_cell_cycle were differentially enriched in phenotypes with high LACTB expression. In addition, CDC20, CDK4, MCM6, MAD2L1, MCM2 and MCM5 were leading genes intersecting in these four pathways, and a positive correlation between mRNA expression and LACTB was observed in most normal and cancer tissues. Finally, elevated LACTB mRNA expression was significantly related to multiple immune marker sets. Our results elucidate that LACTB is involved in the development of cancer, and that high LACTB expression in patients with PAAD can predict a poor prognosis. High LACTB expression was significantly correlated with cell cycle-related genes and multiple immune marker sets.
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spelling pubmed-78429072021-02-02 LACTB mRNA expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis Xie, Jian Peng, Yang Chen, Xiaoyu Li, Qigang Jian, Bin Wen, Zelin Liu, Shengchun PLoS One Research Article This study aimed to find the prognostic value of Beta-lactamase-like (LACTB) in pancreatic adenocarcinoma (PAAD) patients. The mRNA expression of LACTB was upregulated in PAAD and was correlated with vital status (P = 0.0199). The immunoreactive scores of LACTB protein in human PAAD tissues were significantly higher than those in adjacent noncancerous pancreatic tissues. Receiver operating characteristic (ROC) curve assessment showed that LACTB mRNA expression has high diagnostic value in PAAD. Kaplan-Meier curve and Cox analyses suggested that patients with high LACTB mRNA expression have a poor prognosis, indicating that LACTB mRNA is an independent prognostic factor for overall survival [hazard ratio (HR) = 1.72, P = 0.015, 95% confidence interval (CI) = 1.106–2.253] and disease-specific survival (HR = 1.97, P = 0.004, 95% CI = 1.238–3.152) of PAAD patients. Gene set enrichment analysis (GSEA) revealed that hallmark_g2m_checkpoint, hallmark_myc_targets_v1, hallmark_e2f_targets, and kegg_cell_cycle were differentially enriched in phenotypes with high LACTB expression. In addition, CDC20, CDK4, MCM6, MAD2L1, MCM2 and MCM5 were leading genes intersecting in these four pathways, and a positive correlation between mRNA expression and LACTB was observed in most normal and cancer tissues. Finally, elevated LACTB mRNA expression was significantly related to multiple immune marker sets. Our results elucidate that LACTB is involved in the development of cancer, and that high LACTB expression in patients with PAAD can predict a poor prognosis. High LACTB expression was significantly correlated with cell cycle-related genes and multiple immune marker sets. Public Library of Science 2021-01-28 /pmc/articles/PMC7842907/ /pubmed/33507917 http://dx.doi.org/10.1371/journal.pone.0245908 Text en © 2021 Xie et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Xie, Jian
Peng, Yang
Chen, Xiaoyu
Li, Qigang
Jian, Bin
Wen, Zelin
Liu, Shengchun
LACTB mRNA expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis
title LACTB mRNA expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis
title_full LACTB mRNA expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis
title_fullStr LACTB mRNA expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis
title_full_unstemmed LACTB mRNA expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis
title_short LACTB mRNA expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis
title_sort lactb mrna expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842907/
https://www.ncbi.nlm.nih.gov/pubmed/33507917
http://dx.doi.org/10.1371/journal.pone.0245908
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