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Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones

Researchers must be able to measure concentrations, sizes, and infectivity of virus-containing particles in animal agriculture facilities to know how far infectious virus-containing particles may travel through air, where they may deposit in the human or animal respiratory tract, and the most effect...

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Autores principales: Raynor, Peter C., Adesina, Adepeju, Aboubakr, Hamada A., Yang, My, Torremorell, Montserrat, Goyal, Sagar M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842955/
https://www.ncbi.nlm.nih.gov/pubmed/33507951
http://dx.doi.org/10.1371/journal.pone.0244977
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author Raynor, Peter C.
Adesina, Adepeju
Aboubakr, Hamada A.
Yang, My
Torremorell, Montserrat
Goyal, Sagar M.
author_facet Raynor, Peter C.
Adesina, Adepeju
Aboubakr, Hamada A.
Yang, My
Torremorell, Montserrat
Goyal, Sagar M.
author_sort Raynor, Peter C.
collection PubMed
description Researchers must be able to measure concentrations, sizes, and infectivity of virus-containing particles in animal agriculture facilities to know how far infectious virus-containing particles may travel through air, where they may deposit in the human or animal respiratory tract, and the most effective ways to limit exposures to them. The objective of this study was to evaluate a variety of impinger and cyclone aerosol or bioaerosol samplers to determine approaches most suitable for detecting and measuring concentrations of virus-containing particles in air. Six impinger/cyclone air samplers, a filter-based sampler, and a cascade impactor were used in separate tests to collect artificially generated aerosols of MS2 bacteriophage and swine and avian influenza viruses. Quantification of infectious MS2 coliphage was carried out using a double agar layer procedure. The influenza viruses were titrated in cell cultures to determine quantities of infectious virus. Viral RNA was extracted and used for quantitative real time RT-PCR, to provide total virus concentrations for all three viruses. The amounts of virus recovered and the measured airborne virus concentrations were calculated and compared among the samplers. Not surprisingly, high flow rate samplers generally collected greater quantities of virus than low flow samplers. However, low flow rate samplers generally measured higher, and likely more accurate, airborne concentrations of Infectious virus and viral RNA than high flow samplers. To assess airborne viruses in the field, a two-sampler approach may work well. A suitable high flow sampler may provide low limits of detection to determine if any virus is present in the air. If virus is detected, a suitable lower flow sampler may measure airborne virus concentrations accurately.
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spelling pubmed-78429552021-02-04 Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones Raynor, Peter C. Adesina, Adepeju Aboubakr, Hamada A. Yang, My Torremorell, Montserrat Goyal, Sagar M. PLoS One Research Article Researchers must be able to measure concentrations, sizes, and infectivity of virus-containing particles in animal agriculture facilities to know how far infectious virus-containing particles may travel through air, where they may deposit in the human or animal respiratory tract, and the most effective ways to limit exposures to them. The objective of this study was to evaluate a variety of impinger and cyclone aerosol or bioaerosol samplers to determine approaches most suitable for detecting and measuring concentrations of virus-containing particles in air. Six impinger/cyclone air samplers, a filter-based sampler, and a cascade impactor were used in separate tests to collect artificially generated aerosols of MS2 bacteriophage and swine and avian influenza viruses. Quantification of infectious MS2 coliphage was carried out using a double agar layer procedure. The influenza viruses were titrated in cell cultures to determine quantities of infectious virus. Viral RNA was extracted and used for quantitative real time RT-PCR, to provide total virus concentrations for all three viruses. The amounts of virus recovered and the measured airborne virus concentrations were calculated and compared among the samplers. Not surprisingly, high flow rate samplers generally collected greater quantities of virus than low flow samplers. However, low flow rate samplers generally measured higher, and likely more accurate, airborne concentrations of Infectious virus and viral RNA than high flow samplers. To assess airborne viruses in the field, a two-sampler approach may work well. A suitable high flow sampler may provide low limits of detection to determine if any virus is present in the air. If virus is detected, a suitable lower flow sampler may measure airborne virus concentrations accurately. Public Library of Science 2021-01-28 /pmc/articles/PMC7842955/ /pubmed/33507951 http://dx.doi.org/10.1371/journal.pone.0244977 Text en © 2021 Raynor et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Raynor, Peter C.
Adesina, Adepeju
Aboubakr, Hamada A.
Yang, My
Torremorell, Montserrat
Goyal, Sagar M.
Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones
title Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones
title_full Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones
title_fullStr Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones
title_full_unstemmed Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones
title_short Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones
title_sort comparison of samplers collecting airborne influenza viruses: 1. primarily impingers and cyclones
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842955/
https://www.ncbi.nlm.nih.gov/pubmed/33507951
http://dx.doi.org/10.1371/journal.pone.0244977
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