Cargando…
Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones
Researchers must be able to measure concentrations, sizes, and infectivity of virus-containing particles in animal agriculture facilities to know how far infectious virus-containing particles may travel through air, where they may deposit in the human or animal respiratory tract, and the most effect...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842955/ https://www.ncbi.nlm.nih.gov/pubmed/33507951 http://dx.doi.org/10.1371/journal.pone.0244977 |
_version_ | 1783644046183366656 |
---|---|
author | Raynor, Peter C. Adesina, Adepeju Aboubakr, Hamada A. Yang, My Torremorell, Montserrat Goyal, Sagar M. |
author_facet | Raynor, Peter C. Adesina, Adepeju Aboubakr, Hamada A. Yang, My Torremorell, Montserrat Goyal, Sagar M. |
author_sort | Raynor, Peter C. |
collection | PubMed |
description | Researchers must be able to measure concentrations, sizes, and infectivity of virus-containing particles in animal agriculture facilities to know how far infectious virus-containing particles may travel through air, where they may deposit in the human or animal respiratory tract, and the most effective ways to limit exposures to them. The objective of this study was to evaluate a variety of impinger and cyclone aerosol or bioaerosol samplers to determine approaches most suitable for detecting and measuring concentrations of virus-containing particles in air. Six impinger/cyclone air samplers, a filter-based sampler, and a cascade impactor were used in separate tests to collect artificially generated aerosols of MS2 bacteriophage and swine and avian influenza viruses. Quantification of infectious MS2 coliphage was carried out using a double agar layer procedure. The influenza viruses were titrated in cell cultures to determine quantities of infectious virus. Viral RNA was extracted and used for quantitative real time RT-PCR, to provide total virus concentrations for all three viruses. The amounts of virus recovered and the measured airborne virus concentrations were calculated and compared among the samplers. Not surprisingly, high flow rate samplers generally collected greater quantities of virus than low flow samplers. However, low flow rate samplers generally measured higher, and likely more accurate, airborne concentrations of Infectious virus and viral RNA than high flow samplers. To assess airborne viruses in the field, a two-sampler approach may work well. A suitable high flow sampler may provide low limits of detection to determine if any virus is present in the air. If virus is detected, a suitable lower flow sampler may measure airborne virus concentrations accurately. |
format | Online Article Text |
id | pubmed-7842955 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78429552021-02-04 Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones Raynor, Peter C. Adesina, Adepeju Aboubakr, Hamada A. Yang, My Torremorell, Montserrat Goyal, Sagar M. PLoS One Research Article Researchers must be able to measure concentrations, sizes, and infectivity of virus-containing particles in animal agriculture facilities to know how far infectious virus-containing particles may travel through air, where they may deposit in the human or animal respiratory tract, and the most effective ways to limit exposures to them. The objective of this study was to evaluate a variety of impinger and cyclone aerosol or bioaerosol samplers to determine approaches most suitable for detecting and measuring concentrations of virus-containing particles in air. Six impinger/cyclone air samplers, a filter-based sampler, and a cascade impactor were used in separate tests to collect artificially generated aerosols of MS2 bacteriophage and swine and avian influenza viruses. Quantification of infectious MS2 coliphage was carried out using a double agar layer procedure. The influenza viruses were titrated in cell cultures to determine quantities of infectious virus. Viral RNA was extracted and used for quantitative real time RT-PCR, to provide total virus concentrations for all three viruses. The amounts of virus recovered and the measured airborne virus concentrations were calculated and compared among the samplers. Not surprisingly, high flow rate samplers generally collected greater quantities of virus than low flow samplers. However, low flow rate samplers generally measured higher, and likely more accurate, airborne concentrations of Infectious virus and viral RNA than high flow samplers. To assess airborne viruses in the field, a two-sampler approach may work well. A suitable high flow sampler may provide low limits of detection to determine if any virus is present in the air. If virus is detected, a suitable lower flow sampler may measure airborne virus concentrations accurately. Public Library of Science 2021-01-28 /pmc/articles/PMC7842955/ /pubmed/33507951 http://dx.doi.org/10.1371/journal.pone.0244977 Text en © 2021 Raynor et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Raynor, Peter C. Adesina, Adepeju Aboubakr, Hamada A. Yang, My Torremorell, Montserrat Goyal, Sagar M. Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones |
title | Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones |
title_full | Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones |
title_fullStr | Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones |
title_full_unstemmed | Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones |
title_short | Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones |
title_sort | comparison of samplers collecting airborne influenza viruses: 1. primarily impingers and cyclones |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842955/ https://www.ncbi.nlm.nih.gov/pubmed/33507951 http://dx.doi.org/10.1371/journal.pone.0244977 |
work_keys_str_mv | AT raynorpeterc comparisonofsamplerscollectingairborneinfluenzaviruses1primarilyimpingersandcyclones AT adesinaadepeju comparisonofsamplerscollectingairborneinfluenzaviruses1primarilyimpingersandcyclones AT aboubakrhamadaa comparisonofsamplerscollectingairborneinfluenzaviruses1primarilyimpingersandcyclones AT yangmy comparisonofsamplerscollectingairborneinfluenzaviruses1primarilyimpingersandcyclones AT torremorellmontserrat comparisonofsamplerscollectingairborneinfluenzaviruses1primarilyimpingersandcyclones AT goyalsagarm comparisonofsamplerscollectingairborneinfluenzaviruses1primarilyimpingersandcyclones |