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Choroidal congestion mouse model: Could it serve as a pachychoroid model?

Pachychoroid spectrum diseases have been described as a new clinical entity within the spectrum of macular disorders. “Pachychoroid” is defined as choroidal thickening associated with dilated outer choroidal vessels often showing retinal pigment epithelium (RPE) degeneration. Although various clinic...

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Autores principales: Matsumoto, Hidetaka, Mukai, Ryo, Hoshino, Junki, Oda, Mai, Matsuzaki, Toshiyuki, Ishizaki, Yasuki, Shibasaki, Koji, Akiyama, Hideo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843010/
https://www.ncbi.nlm.nih.gov/pubmed/33507997
http://dx.doi.org/10.1371/journal.pone.0246115
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author Matsumoto, Hidetaka
Mukai, Ryo
Hoshino, Junki
Oda, Mai
Matsuzaki, Toshiyuki
Ishizaki, Yasuki
Shibasaki, Koji
Akiyama, Hideo
author_facet Matsumoto, Hidetaka
Mukai, Ryo
Hoshino, Junki
Oda, Mai
Matsuzaki, Toshiyuki
Ishizaki, Yasuki
Shibasaki, Koji
Akiyama, Hideo
author_sort Matsumoto, Hidetaka
collection PubMed
description Pachychoroid spectrum diseases have been described as a new clinical entity within the spectrum of macular disorders. “Pachychoroid” is defined as choroidal thickening associated with dilated outer choroidal vessels often showing retinal pigment epithelium (RPE) degeneration. Although various clinical studies on the pachychoroid spectrum diseases have been conducted, the pathophysiology of pachychoroid has yet to be fully elucidated. In this study, we attempted to establish a mouse model of pachychoroid. We sutured vortex veins in eyes of wild type mice to imitate the vortex vein congestion in pachychoroid spectrum diseases. Fundus photography and ultra-widefield indocyanine green angiography showed dilated vortex veins from the posterior pole to the ampulla in eyes after induction of choroidal congestion. Optical coherence tomography and tissue sections presented choroidal thickening with dilatation of choroidal vessels. The RPE-choroid/retina thickness ratios on the tissue sections in the treated day 1 and day 7 groups were significantly greater than that in the control group (0.19±0.03 and 0.16±0.01 vs. 0.12±0.02, P<0.05 each). Moreover, immunohistochemistry using RPE flatmount revealed focal RPE degeneration in the treated eyes. Furthermore, inflammatory response-related genes were upregulated in eyes with choroidal congestion induction, and macrophages migrated into the thickened choroid. These results indicated that vortex vein congestion triggered some pachychoroid features. Thus, we have established a choroidal congestion mouse model by suturing vortex veins, which would potentially be useful for investigating the pathophysiology of pachychoroid spectrum diseases.
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spelling pubmed-78430102021-02-04 Choroidal congestion mouse model: Could it serve as a pachychoroid model? Matsumoto, Hidetaka Mukai, Ryo Hoshino, Junki Oda, Mai Matsuzaki, Toshiyuki Ishizaki, Yasuki Shibasaki, Koji Akiyama, Hideo PLoS One Research Article Pachychoroid spectrum diseases have been described as a new clinical entity within the spectrum of macular disorders. “Pachychoroid” is defined as choroidal thickening associated with dilated outer choroidal vessels often showing retinal pigment epithelium (RPE) degeneration. Although various clinical studies on the pachychoroid spectrum diseases have been conducted, the pathophysiology of pachychoroid has yet to be fully elucidated. In this study, we attempted to establish a mouse model of pachychoroid. We sutured vortex veins in eyes of wild type mice to imitate the vortex vein congestion in pachychoroid spectrum diseases. Fundus photography and ultra-widefield indocyanine green angiography showed dilated vortex veins from the posterior pole to the ampulla in eyes after induction of choroidal congestion. Optical coherence tomography and tissue sections presented choroidal thickening with dilatation of choroidal vessels. The RPE-choroid/retina thickness ratios on the tissue sections in the treated day 1 and day 7 groups were significantly greater than that in the control group (0.19±0.03 and 0.16±0.01 vs. 0.12±0.02, P<0.05 each). Moreover, immunohistochemistry using RPE flatmount revealed focal RPE degeneration in the treated eyes. Furthermore, inflammatory response-related genes were upregulated in eyes with choroidal congestion induction, and macrophages migrated into the thickened choroid. These results indicated that vortex vein congestion triggered some pachychoroid features. Thus, we have established a choroidal congestion mouse model by suturing vortex veins, which would potentially be useful for investigating the pathophysiology of pachychoroid spectrum diseases. Public Library of Science 2021-01-28 /pmc/articles/PMC7843010/ /pubmed/33507997 http://dx.doi.org/10.1371/journal.pone.0246115 Text en © 2021 Matsumoto et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Matsumoto, Hidetaka
Mukai, Ryo
Hoshino, Junki
Oda, Mai
Matsuzaki, Toshiyuki
Ishizaki, Yasuki
Shibasaki, Koji
Akiyama, Hideo
Choroidal congestion mouse model: Could it serve as a pachychoroid model?
title Choroidal congestion mouse model: Could it serve as a pachychoroid model?
title_full Choroidal congestion mouse model: Could it serve as a pachychoroid model?
title_fullStr Choroidal congestion mouse model: Could it serve as a pachychoroid model?
title_full_unstemmed Choroidal congestion mouse model: Could it serve as a pachychoroid model?
title_short Choroidal congestion mouse model: Could it serve as a pachychoroid model?
title_sort choroidal congestion mouse model: could it serve as a pachychoroid model?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843010/
https://www.ncbi.nlm.nih.gov/pubmed/33507997
http://dx.doi.org/10.1371/journal.pone.0246115
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